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Evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells
Although mesenchymal stem cells (MSCs) have exhibited promising immunomodulatory potential in preclinical studies, clinical studies have revealed variable results. These results often depend on environmental cues. Pre-conditioning MSCs with cytokines is one of the methods used to enhance their immun...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Libbey Eurotext
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134695/ https://www.ncbi.nlm.nih.gov/pubmed/37052151 http://dx.doi.org/10.1684/ecn.2022.0482 |
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author | Bayati, Fatemeh Valadi, Maryam Ahmadi, Armin Najafi, Farangis Ansaripour, Bita Sharif-Paghaleh, Ehsan |
author_facet | Bayati, Fatemeh Valadi, Maryam Ahmadi, Armin Najafi, Farangis Ansaripour, Bita Sharif-Paghaleh, Ehsan |
author_sort | Bayati, Fatemeh |
collection | PubMed |
description | Although mesenchymal stem cells (MSCs) have exhibited promising immunomodulatory potential in preclinical studies, clinical studies have revealed variable results. These results often depend on environmental cues. Pre-conditioning MSCs with cytokines is one of the methods used to enhance their immunomodulatory effects. In this study, we harvested adipose-derived MSCs from mice and cultured them with different doses of the cytokine, IFN-γ, and the corticosteroid drug, dexamethasone, in order to investigate their effects on MSC immunosuppressive function. We found the co-culture or supernatant of MSCs, pre-conditioned with IFN-γ, together with spleen mononuclear cells resulted in a significant reduction of mononuclear cell proliferation. Although the supernatant of MSCs, pre-conditioned with dexamethasone, showed similar results, dexamethasone pre-conditioning of co-cultured MSCs increased mononuclear cell proliferation. The results further our understanding of immune-related effects of MSCs which may provide a basis for further in vivo studies to achieve better clinical results. We propose that pre-conditioning with cytokines might be an effective method to boost the immunomodulatory effects of MSCs. |
format | Online Article Text |
id | pubmed-10134695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Libbey Eurotext |
record_format | MEDLINE/PubMed |
spelling | pubmed-101346952023-04-28 Evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells Bayati, Fatemeh Valadi, Maryam Ahmadi, Armin Najafi, Farangis Ansaripour, Bita Sharif-Paghaleh, Ehsan Eur Cytokine Netw Research Article Although mesenchymal stem cells (MSCs) have exhibited promising immunomodulatory potential in preclinical studies, clinical studies have revealed variable results. These results often depend on environmental cues. Pre-conditioning MSCs with cytokines is one of the methods used to enhance their immunomodulatory effects. In this study, we harvested adipose-derived MSCs from mice and cultured them with different doses of the cytokine, IFN-γ, and the corticosteroid drug, dexamethasone, in order to investigate their effects on MSC immunosuppressive function. We found the co-culture or supernatant of MSCs, pre-conditioned with IFN-γ, together with spleen mononuclear cells resulted in a significant reduction of mononuclear cell proliferation. Although the supernatant of MSCs, pre-conditioned with dexamethasone, showed similar results, dexamethasone pre-conditioning of co-cultured MSCs increased mononuclear cell proliferation. The results further our understanding of immune-related effects of MSCs which may provide a basis for further in vivo studies to achieve better clinical results. We propose that pre-conditioning with cytokines might be an effective method to boost the immunomodulatory effects of MSCs. John Libbey Eurotext 2023-04-27 2022 /pmc/articles/PMC10134695/ /pubmed/37052151 http://dx.doi.org/10.1684/ecn.2022.0482 Text en © JLE/Springer 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Research Article Bayati, Fatemeh Valadi, Maryam Ahmadi, Armin Najafi, Farangis Ansaripour, Bita Sharif-Paghaleh, Ehsan Evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells |
title | Evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells |
title_full | Evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells |
title_fullStr | Evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells |
title_full_unstemmed | Evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells |
title_short | Evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells |
title_sort | evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or ifn-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134695/ https://www.ncbi.nlm.nih.gov/pubmed/37052151 http://dx.doi.org/10.1684/ecn.2022.0482 |
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