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Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail
We explore the relationship between the melanophilin (MLPH) gene and quail plumage color and provide a reference for subsequent quail plumage color breeding. In this experiment, real-time quantitative PCR (RT-qPCR) technology was used to analyze the relative mRNA expression levels of Korean quail (m...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Copernicus GmbH
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134764/ https://www.ncbi.nlm.nih.gov/pubmed/37124941 http://dx.doi.org/10.5194/aab-66-131-2023 |
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author | Yuan, Zhiwen Zhang, Xiaohui Pang, Youzhi Qi, Yanxia Wang, Qiankun Hu, Yunqi Zhao, Yiwei Ren, Shiwei Huo, Linke |
author_facet | Yuan, Zhiwen Zhang, Xiaohui Pang, Youzhi Qi, Yanxia Wang, Qiankun Hu, Yunqi Zhao, Yiwei Ren, Shiwei Huo, Linke |
author_sort | Yuan, Zhiwen |
collection | PubMed |
description | We explore the relationship between the melanophilin (MLPH) gene and quail plumage color and provide a reference for subsequent quail plumage color breeding. In this experiment, real-time quantitative PCR (RT-qPCR) technology was used to analyze the relative mRNA expression levels of Korean quail (maroon) and Beijing white quail embryos at different developmental stages. Two single-nucleotide polymorphisms (SNPs) in the MLPH gene were screened based on the RNA-sequencing (RNA-Seq) data of skin tissues of Korean quail and Beijing white quail during the embryonic stage. Kompetitive allele-specific PCR (KASP) technology was used for genotyping in the resource population, and correlation analysis was carried out with the plumage color traits of quail. Finally, bioinformatics was used to predict the effects of these two SNPs on the structure and function of the encoded protein. The results showed that the expression level of the MLPH gene during embryonic development of Beijing white quail was significantly higher than that of Korean quail ( [Formula: see text] ). The frequency distribution of the three genotypes (CC, CA and AA) of the Beijing white quail at the c.1807C [Formula: see text] A mutation site was significantly different from that of the Korean quail ( [Formula: see text] ). The frequency distribution of the three genotypes (GG, GA and AA) of the Beijing white quail at the c.2129G [Formula: see text] A mutation site was significantly different from that of the Korean quail ( [Formula: see text] ). And there was a significant correlation between the c.1807C [Formula: see text] A mutation site and the white plumage phenotype. Bioinformatics showed that SNP1 (c.1807C [Formula: see text] A) was a neutral mutation and that SNP2 (c.2129G [Formula: see text] A) was a deleterious mutation. The prediction of protein conservation showed that the mutation sites of coding proteins R603S and G710D caused by SNP1 (c.1807C [Formula: see text] A) and SNP2 (c.2129G [Formula: see text] A) were highly conserved. |
format | Online Article Text |
id | pubmed-10134764 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Copernicus GmbH |
record_format | MEDLINE/PubMed |
spelling | pubmed-101347642023-04-28 Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail Yuan, Zhiwen Zhang, Xiaohui Pang, Youzhi Qi, Yanxia Wang, Qiankun Hu, Yunqi Zhao, Yiwei Ren, Shiwei Huo, Linke Arch Anim Breed Original Study We explore the relationship between the melanophilin (MLPH) gene and quail plumage color and provide a reference for subsequent quail plumage color breeding. In this experiment, real-time quantitative PCR (RT-qPCR) technology was used to analyze the relative mRNA expression levels of Korean quail (maroon) and Beijing white quail embryos at different developmental stages. Two single-nucleotide polymorphisms (SNPs) in the MLPH gene were screened based on the RNA-sequencing (RNA-Seq) data of skin tissues of Korean quail and Beijing white quail during the embryonic stage. Kompetitive allele-specific PCR (KASP) technology was used for genotyping in the resource population, and correlation analysis was carried out with the plumage color traits of quail. Finally, bioinformatics was used to predict the effects of these two SNPs on the structure and function of the encoded protein. The results showed that the expression level of the MLPH gene during embryonic development of Beijing white quail was significantly higher than that of Korean quail ( [Formula: see text] ). The frequency distribution of the three genotypes (CC, CA and AA) of the Beijing white quail at the c.1807C [Formula: see text] A mutation site was significantly different from that of the Korean quail ( [Formula: see text] ). The frequency distribution of the three genotypes (GG, GA and AA) of the Beijing white quail at the c.2129G [Formula: see text] A mutation site was significantly different from that of the Korean quail ( [Formula: see text] ). And there was a significant correlation between the c.1807C [Formula: see text] A mutation site and the white plumage phenotype. Bioinformatics showed that SNP1 (c.1807C [Formula: see text] A) was a neutral mutation and that SNP2 (c.2129G [Formula: see text] A) was a deleterious mutation. The prediction of protein conservation showed that the mutation sites of coding proteins R603S and G710D caused by SNP1 (c.1807C [Formula: see text] A) and SNP2 (c.2129G [Formula: see text] A) were highly conserved. Copernicus GmbH 2023-03-22 /pmc/articles/PMC10134764/ /pubmed/37124941 http://dx.doi.org/10.5194/aab-66-131-2023 Text en Copyright: © 2023 Zhiwen Yuan et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Original Study Yuan, Zhiwen Zhang, Xiaohui Pang, Youzhi Qi, Yanxia Wang, Qiankun Hu, Yunqi Zhao, Yiwei Ren, Shiwei Huo, Linke Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail |
title | Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail |
title_full | Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail |
title_fullStr | Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail |
title_full_unstemmed | Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail |
title_short | Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail |
title_sort | association analysis of melanophilin (mlph) gene expression and polymorphism with plumage color in quail |
topic | Original Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134764/ https://www.ncbi.nlm.nih.gov/pubmed/37124941 http://dx.doi.org/10.5194/aab-66-131-2023 |
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