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Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4‐mediated NF‐κB signaling pathway

INTRODUCTION: Chronic obstructive pulmonary disease (COPD) is associated with chronic inflammation that predominantly affects the lung and peripheral airways. Previous investigation has underlined the efficacy of luteolin in the treatment of inflammation‐related symptoms. Accordingly, our study conc...

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Autores principales: Li, Mingfei, Wang, Huifang, Lu, Yun, Cai, Jinwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134768/
https://www.ncbi.nlm.nih.gov/pubmed/37102667
http://dx.doi.org/10.1002/iid3.820
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author Li, Mingfei
Wang, Huifang
Lu, Yun
Cai, Jinwen
author_facet Li, Mingfei
Wang, Huifang
Lu, Yun
Cai, Jinwen
author_sort Li, Mingfei
collection PubMed
description INTRODUCTION: Chronic obstructive pulmonary disease (COPD) is associated with chronic inflammation that predominantly affects the lung and peripheral airways. Previous investigation has underlined the efficacy of luteolin in the treatment of inflammation‐related symptoms. Accordingly, our study concentrates on unveiling the effect of luteolin on COPD. METHODS: Mice or A549 cells were treated with cigarette smoke (CS) to establish COPD models in vivo and in vitro. Then, the serum and bronchoalveolar lavage fluid of mice were harvested. The lung tissues of mice were subjected to hematoxylin‐eosin staining to observe the degree of damage. The inflammation and oxidative stress factors level were calculated via enzyme‐linked immunosorbent assay and quantitative real‐time polymerase chain reaction. The expressions of nuclear factor‐kappa B (NF‐κB) pathway‐related factors were detected by Western blot. RESULTS: In in vivo experiments, CS treatment reduced the weight of mice and promoted lung tissue damage, while luteolin attenuated the effect of CS on the mice. Moreover, luteolin inhibited the inflammation factors level, oxidative stress, and NADPH oxidase 4 (NOX4)‐mediated NF‐κB signaling pathway in CS‐induced COPD mice. Similar results were obtained in in vitro experiments that luteolin alleviated CS‐induced inflammation, oxidative stress, and NOX4‐mediated NF‐κB signaling pathway activation in CS‐treated A549 cells. Besides, NOX4 overexpression offset the impacts of luteolin on the CS‐induced A549 cells. CONCLUSION: Luteolin alleviates inflammation and oxidative stress in COPD via NOX4‐mediated NF‐κB signaling pathway, which provides a theoretical basis for the treatment of COPD with luteolin.
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spelling pubmed-101347682023-04-28 Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4‐mediated NF‐κB signaling pathway Li, Mingfei Wang, Huifang Lu, Yun Cai, Jinwen Immun Inflamm Dis Original Articles INTRODUCTION: Chronic obstructive pulmonary disease (COPD) is associated with chronic inflammation that predominantly affects the lung and peripheral airways. Previous investigation has underlined the efficacy of luteolin in the treatment of inflammation‐related symptoms. Accordingly, our study concentrates on unveiling the effect of luteolin on COPD. METHODS: Mice or A549 cells were treated with cigarette smoke (CS) to establish COPD models in vivo and in vitro. Then, the serum and bronchoalveolar lavage fluid of mice were harvested. The lung tissues of mice were subjected to hematoxylin‐eosin staining to observe the degree of damage. The inflammation and oxidative stress factors level were calculated via enzyme‐linked immunosorbent assay and quantitative real‐time polymerase chain reaction. The expressions of nuclear factor‐kappa B (NF‐κB) pathway‐related factors were detected by Western blot. RESULTS: In in vivo experiments, CS treatment reduced the weight of mice and promoted lung tissue damage, while luteolin attenuated the effect of CS on the mice. Moreover, luteolin inhibited the inflammation factors level, oxidative stress, and NADPH oxidase 4 (NOX4)‐mediated NF‐κB signaling pathway in CS‐induced COPD mice. Similar results were obtained in in vitro experiments that luteolin alleviated CS‐induced inflammation, oxidative stress, and NOX4‐mediated NF‐κB signaling pathway activation in CS‐treated A549 cells. Besides, NOX4 overexpression offset the impacts of luteolin on the CS‐induced A549 cells. CONCLUSION: Luteolin alleviates inflammation and oxidative stress in COPD via NOX4‐mediated NF‐κB signaling pathway, which provides a theoretical basis for the treatment of COPD with luteolin. John Wiley and Sons Inc. 2023-04-27 /pmc/articles/PMC10134768/ /pubmed/37102667 http://dx.doi.org/10.1002/iid3.820 Text en © 2023 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Li, Mingfei
Wang, Huifang
Lu, Yun
Cai, Jinwen
Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4‐mediated NF‐κB signaling pathway
title Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4‐mediated NF‐κB signaling pathway
title_full Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4‐mediated NF‐κB signaling pathway
title_fullStr Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4‐mediated NF‐κB signaling pathway
title_full_unstemmed Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4‐mediated NF‐κB signaling pathway
title_short Luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the NOX4‐mediated NF‐κB signaling pathway
title_sort luteolin suppresses inflammation and oxidative stress in chronic obstructive pulmonary disease through inhibition of the nox4‐mediated nf‐κb signaling pathway
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134768/
https://www.ncbi.nlm.nih.gov/pubmed/37102667
http://dx.doi.org/10.1002/iid3.820
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