Systematic Evaluation of the Viable Microbiome in the Human Oral and Gut Samples with Spike-in Gram+/– Bacteria

PMA (propidium monoazide) is one of the few methods that are compatible with metagenomic sequencing to characterize the live/intact microbiota. However, its efficiency in complex communities such as saliva and feces is still controversial. An effective method for depleting host and dead bacterial DN...

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Detalles Bibliográficos
Autores principales: Liu, Feng, Lu, Hui, Dong, Biao, Huang, Xiaochang, Cheng, Hongyu, Qu, Ru, Hu, Yichen, Zhong, Luyun, Guo, Zhenni, You, Yuehua, Xu, Zhenjiang Zech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134872/
https://www.ncbi.nlm.nih.gov/pubmed/36971593
http://dx.doi.org/10.1128/msystems.00738-22

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134872/
https://www.ncbi.nlm.nih.gov/pubmed/36971593
http://dx.doi.org/10.1128/msystems.00738-22

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