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Simultaneous determination of nine phenolic compounds in imitation wild Dendrobium officinale samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry

Dendrobium officinale Kimura et Migo (D. officinale), one of the nine everlasting types of grass, has gained increasing attention owing to its important roles in alternative medicines and drug discovery. Due to its natural resources being in danger of being extinct, imitation wild planting is becomi...

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Autores principales: Mu, Yingsu, Cheng, Li, Gong, Xiaojian, Ma, Jiangxiong, Zhang, Shiyu, Mu, Yinghua, Liang, Kang, Zhou, Xin, Zhao, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134899/
https://www.ncbi.nlm.nih.gov/pubmed/37125030
http://dx.doi.org/10.3389/fnut.2023.1129953
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author Mu, Yingsu
Cheng, Li
Gong, Xiaojian
Ma, Jiangxiong
Zhang, Shiyu
Mu, Yinghua
Liang, Kang
Zhou, Xin
Zhao, Chao
author_facet Mu, Yingsu
Cheng, Li
Gong, Xiaojian
Ma, Jiangxiong
Zhang, Shiyu
Mu, Yinghua
Liang, Kang
Zhou, Xin
Zhao, Chao
author_sort Mu, Yingsu
collection PubMed
description Dendrobium officinale Kimura et Migo (D. officinale), one of the nine everlasting types of grass, has gained increasing attention owing to its important roles in alternative medicines and drug discovery. Due to its natural resources being in danger of being extinct, imitation wild planting is becoming increasingly common. To assess the product’s quality completely, an efficient ultrahigh performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-QQQ-MS/MS) method was established to simultaneously quantify nine phenolic compounds in D. officinale samples. The extraction parameters, including solvent, solvent concentration, solid–liquid ratio, and extraction time, were systematically optimized with the single-factor test. The results demonstrated that extraction with a 1:200 solid-to-liquid ratio of 80% methanol for 1.5 h was the most efficient condition for the extraction of flavonoids. Satisfactory retention times and resolution of the nine analytes were acquired on the Thermo Scientific Hypersil GOLD column with multiple reaction monitoring in negative ion scanning mode. The method was validated to demonstrate its selectivity, linearity, precision, accuracy, and robustness. Thus, the verified UHPLC-QQQ-MS/MS method was successfully applied to the quantification of phenolic components present in D. officinale samples. The results indicated that the quantity and composition of phenolic components in D. officinale from various provenances were significantly different. This work provides a theoretical foundation for the cultivation and assessment of wild D. officinale quality.
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spelling pubmed-101348992023-04-28 Simultaneous determination of nine phenolic compounds in imitation wild Dendrobium officinale samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry Mu, Yingsu Cheng, Li Gong, Xiaojian Ma, Jiangxiong Zhang, Shiyu Mu, Yinghua Liang, Kang Zhou, Xin Zhao, Chao Front Nutr Nutrition Dendrobium officinale Kimura et Migo (D. officinale), one of the nine everlasting types of grass, has gained increasing attention owing to its important roles in alternative medicines and drug discovery. Due to its natural resources being in danger of being extinct, imitation wild planting is becoming increasingly common. To assess the product’s quality completely, an efficient ultrahigh performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-QQQ-MS/MS) method was established to simultaneously quantify nine phenolic compounds in D. officinale samples. The extraction parameters, including solvent, solvent concentration, solid–liquid ratio, and extraction time, were systematically optimized with the single-factor test. The results demonstrated that extraction with a 1:200 solid-to-liquid ratio of 80% methanol for 1.5 h was the most efficient condition for the extraction of flavonoids. Satisfactory retention times and resolution of the nine analytes were acquired on the Thermo Scientific Hypersil GOLD column with multiple reaction monitoring in negative ion scanning mode. The method was validated to demonstrate its selectivity, linearity, precision, accuracy, and robustness. Thus, the verified UHPLC-QQQ-MS/MS method was successfully applied to the quantification of phenolic components present in D. officinale samples. The results indicated that the quantity and composition of phenolic components in D. officinale from various provenances were significantly different. This work provides a theoretical foundation for the cultivation and assessment of wild D. officinale quality. Frontiers Media S.A. 2023-04-13 /pmc/articles/PMC10134899/ /pubmed/37125030 http://dx.doi.org/10.3389/fnut.2023.1129953 Text en Copyright © 2023 Mu, Cheng, Gong, Ma, Zhang, Mu, Liang, Zhou and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Mu, Yingsu
Cheng, Li
Gong, Xiaojian
Ma, Jiangxiong
Zhang, Shiyu
Mu, Yinghua
Liang, Kang
Zhou, Xin
Zhao, Chao
Simultaneous determination of nine phenolic compounds in imitation wild Dendrobium officinale samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry
title Simultaneous determination of nine phenolic compounds in imitation wild Dendrobium officinale samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry
title_full Simultaneous determination of nine phenolic compounds in imitation wild Dendrobium officinale samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry
title_fullStr Simultaneous determination of nine phenolic compounds in imitation wild Dendrobium officinale samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry
title_full_unstemmed Simultaneous determination of nine phenolic compounds in imitation wild Dendrobium officinale samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry
title_short Simultaneous determination of nine phenolic compounds in imitation wild Dendrobium officinale samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry
title_sort simultaneous determination of nine phenolic compounds in imitation wild dendrobium officinale samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134899/
https://www.ncbi.nlm.nih.gov/pubmed/37125030
http://dx.doi.org/10.3389/fnut.2023.1129953
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