On the Species Identification of Two Non-Native Tilapia Species, Including the First Record of a Feral Population of Oreochromis aureus (Steindachner, 1864) in South Korea

SIMPLE SUMMARY: Tilapia is farmed in many parts of the world. Tilapia species have likely been introduced into new habitats, altering these habitats at times detrimentally. In this study, the morphological and molecular identification of the tilapia species inhabiting the Dalseo Stream in South Korea was undertaken. Currently, it is reported that only one species of O. niloticus inhabits natural rivers in Korea. However, except for the aquaculture population, the O. aureus natural population, whose habitat has not been reported in natural rivers, was identified for the first time in this study. The results of this study provide useful data for establishing management plans for invasive species management and the information necessary for identifying tilapia species that exist in Korea. ABSTRACT: Tilapia is an invasive species that has become widely distributed around the world. In Korea, introduced tilapia into its aquatic ecosystem for the first time with a species from Thailand in 1955, and later additionally introduced two more species from Japan and Taiwan, thus securing a total of three species of tilapia (O. niloticus, O. mossambicus and O. aureus) as food resources. Since then, O. niloticus has been reported to inhabit certain streams with thermal effluent outlets. Morphological species identification is very difficult for tilapia and a combined analysis of morphological and molecular-based species identification is therefore necessary. This study investigated a tilapia population that inhabits a thermal effluent stream (Dalseo Stream) in Daegu Metropolitan City, Korea, in order to conduct a morphological and genetic species identification of this population. In total, 37 tilapia individuals were sampled. The results of the morphological and genetic species identification analyses found that two species, O. aureus and O. niloticus, inhabit the Dalseo Stream. In Korea, the habitat of the O. niloticus natural population has been reported, but the O. aureus natural population has not been reported. Thus, we observed for the first time that a new invasive species, O. aureus, inhabits a stream in Korea. They are known to cause disturbances to aquatic organisms (e.g., fish, aquatic insects, plankton, aquatic plants) and the habitat environment (e.g., water quality, bottom structure). Accordingly, it is important to study the ecological effects of O. aureus and O. niloticus on the corresponding freshwater ecosystem closely and to prepare a management plan to prevent the spread of these species, as they are notoriously invasive.