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First Detection of mcr-9 in a Multidrug-Resistant Escherichia coli of Animal Origin in Italy Is Not Related to Colistin Usage on a Pig Farm

The emergence of colistin resistance raises growing concerns because of its use as a last-resort antimicrobial for the treatment of severe gram-negative bacterial infections in humans. Plasmid-borne mobile colistin resistance genes (mcr) are particularly worrisome due to their high propensity to spr...

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Autores principales: Guarneri, Flavia, Bertasio, Cristina, Romeo, Claudia, Formenti, Nicoletta, Scali, Federico, Parisio, Giovanni, Canziani, Sabrina, Boifava, Chiara, Guadagno, Federica, Boniotti, Maria Beatrice, Alborali, Giovanni Loris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134971/
https://www.ncbi.nlm.nih.gov/pubmed/37107051
http://dx.doi.org/10.3390/antibiotics12040689
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author Guarneri, Flavia
Bertasio, Cristina
Romeo, Claudia
Formenti, Nicoletta
Scali, Federico
Parisio, Giovanni
Canziani, Sabrina
Boifava, Chiara
Guadagno, Federica
Boniotti, Maria Beatrice
Alborali, Giovanni Loris
author_facet Guarneri, Flavia
Bertasio, Cristina
Romeo, Claudia
Formenti, Nicoletta
Scali, Federico
Parisio, Giovanni
Canziani, Sabrina
Boifava, Chiara
Guadagno, Federica
Boniotti, Maria Beatrice
Alborali, Giovanni Loris
author_sort Guarneri, Flavia
collection PubMed
description The emergence of colistin resistance raises growing concerns because of its use as a last-resort antimicrobial for the treatment of severe gram-negative bacterial infections in humans. Plasmid-borne mobile colistin resistance genes (mcr) are particularly worrisome due to their high propensity to spread. An mcr-9-positive Escherichia coli was isolated from a piglet in Italy, representing the first isolation of this gene from an E. coli of animal origin in the country. Whole genome sequencing (WGS) revealed that mcr-9 was borne by an IncHI2 plasmid carrying several other resistance genes. The strain was indeed phenotypically resistant to six different antimicrobial classes, including 3rd and 4th generation cephalosporins. Despite the presence of mcr-9, the isolate was susceptible to colistin, probably because of a genetic background unfavourable to mcr-9 expression. The lack of colistin resistance, coupled with the fact that the farm of origin had not used colistin in years, suggests that mcr-9 in such a multidrug-resistant strain can be maintained thanks to the co-selection of neighbouring resistance genes, following usage of different antimicrobials. Our findings highlight how a comprehensive approach, integrating phenotypical testing, targeted PCR, WGS-based techniques, and information on antimicrobial usage is crucial to shed light on antimicrobial resistance.
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spelling pubmed-101349712023-04-28 First Detection of mcr-9 in a Multidrug-Resistant Escherichia coli of Animal Origin in Italy Is Not Related to Colistin Usage on a Pig Farm Guarneri, Flavia Bertasio, Cristina Romeo, Claudia Formenti, Nicoletta Scali, Federico Parisio, Giovanni Canziani, Sabrina Boifava, Chiara Guadagno, Federica Boniotti, Maria Beatrice Alborali, Giovanni Loris Antibiotics (Basel) Article The emergence of colistin resistance raises growing concerns because of its use as a last-resort antimicrobial for the treatment of severe gram-negative bacterial infections in humans. Plasmid-borne mobile colistin resistance genes (mcr) are particularly worrisome due to their high propensity to spread. An mcr-9-positive Escherichia coli was isolated from a piglet in Italy, representing the first isolation of this gene from an E. coli of animal origin in the country. Whole genome sequencing (WGS) revealed that mcr-9 was borne by an IncHI2 plasmid carrying several other resistance genes. The strain was indeed phenotypically resistant to six different antimicrobial classes, including 3rd and 4th generation cephalosporins. Despite the presence of mcr-9, the isolate was susceptible to colistin, probably because of a genetic background unfavourable to mcr-9 expression. The lack of colistin resistance, coupled with the fact that the farm of origin had not used colistin in years, suggests that mcr-9 in such a multidrug-resistant strain can be maintained thanks to the co-selection of neighbouring resistance genes, following usage of different antimicrobials. Our findings highlight how a comprehensive approach, integrating phenotypical testing, targeted PCR, WGS-based techniques, and information on antimicrobial usage is crucial to shed light on antimicrobial resistance. MDPI 2023-04-01 /pmc/articles/PMC10134971/ /pubmed/37107051 http://dx.doi.org/10.3390/antibiotics12040689 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guarneri, Flavia
Bertasio, Cristina
Romeo, Claudia
Formenti, Nicoletta
Scali, Federico
Parisio, Giovanni
Canziani, Sabrina
Boifava, Chiara
Guadagno, Federica
Boniotti, Maria Beatrice
Alborali, Giovanni Loris
First Detection of mcr-9 in a Multidrug-Resistant Escherichia coli of Animal Origin in Italy Is Not Related to Colistin Usage on a Pig Farm
title First Detection of mcr-9 in a Multidrug-Resistant Escherichia coli of Animal Origin in Italy Is Not Related to Colistin Usage on a Pig Farm
title_full First Detection of mcr-9 in a Multidrug-Resistant Escherichia coli of Animal Origin in Italy Is Not Related to Colistin Usage on a Pig Farm
title_fullStr First Detection of mcr-9 in a Multidrug-Resistant Escherichia coli of Animal Origin in Italy Is Not Related to Colistin Usage on a Pig Farm
title_full_unstemmed First Detection of mcr-9 in a Multidrug-Resistant Escherichia coli of Animal Origin in Italy Is Not Related to Colistin Usage on a Pig Farm
title_short First Detection of mcr-9 in a Multidrug-Resistant Escherichia coli of Animal Origin in Italy Is Not Related to Colistin Usage on a Pig Farm
title_sort first detection of mcr-9 in a multidrug-resistant escherichia coli of animal origin in italy is not related to colistin usage on a pig farm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134971/
https://www.ncbi.nlm.nih.gov/pubmed/37107051
http://dx.doi.org/10.3390/antibiotics12040689
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