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Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage

SIMPLE SUMMARY: The Dioscorea alata L. is known to contain abundant proanthocyanidins and anthocyanins, which exhibit potent antioxidant properties capable of scavenging free radicals. Additionally, these compounds have been shown to effectively promote the proliferation of IPEC-J2 cells within a ph...

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Autores principales: Yun, Yanhong, Shi, Huiyu, Wang, Yanyu, Yang, Fengyuan, Zhang, Yuanxin, Feng, Haibo, Chen, Junpu, Wang, Xuemei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135252/
https://www.ncbi.nlm.nih.gov/pubmed/37106964
http://dx.doi.org/10.3390/ani13081401
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author Yun, Yanhong
Shi, Huiyu
Wang, Yanyu
Yang, Fengyuan
Zhang, Yuanxin
Feng, Haibo
Chen, Junpu
Wang, Xuemei
author_facet Yun, Yanhong
Shi, Huiyu
Wang, Yanyu
Yang, Fengyuan
Zhang, Yuanxin
Feng, Haibo
Chen, Junpu
Wang, Xuemei
author_sort Yun, Yanhong
collection PubMed
description SIMPLE SUMMARY: The Dioscorea alata L. is known to contain abundant proanthocyanidins and anthocyanins, which exhibit potent antioxidant properties capable of scavenging free radicals. Additionally, these compounds have been shown to effectively promote the proliferation of IPEC-J2 cells within a pharmacotoxicity range. Conversely, exposure to H(2)O(2) can damage IPEC-J2 cells and trigger increased activity of cellular antioxidant enzymes. This study investigated the impact of crude extract from Dioscorea alata L. on the antioxidant capacity of IPEC-J2 cells and explored the pre-protective mechanism of Dioscorea alata L. against oxidative damage induced by H(2)O(2) through an examination of its effect on the NF-κB inflammatory signaling pathway. ABSTRACT: The purple tubers of Dioscorea alata L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of Dioscorea alata L. and its crude extracts on cells prior to oxidative stress. To establish a suitable oxidative damage model, an injured model of IPEC-J2 cells was created using H(2)O(2) as the oxidant. Specifically, when the concentration of H(2)O(2) was 120 μmol/L and the injured time was 8 h, the survival rate of cells decreased to approximately 70%, and the cells exhibited a noticeable oxidative stress reaction. Moreover, the crude extracts of Dioscorea alata L. demonstrated beneficial pre-protective effects on IPEC-J2 cells by increasing the total antioxidant capacity (T-AOC) and catalase (CAT) activities, augmenting the expression of total superoxide dismutase (T-SOD) and its genes, reducing the content of malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-P(X)) and its expression of genes, and promoting the expression of glucose transporter SGLT1 gene while reducing that of GULT2 gene, thereby facilitating the entry of anthocyanins into cells. In addition, the 50 μg/mL crude extracts effectively inhibited the phosphorylation of IκB and the p65 protein, thus reducing cellular oxidative stress. Given these findings, Dioscorea alata L. can be considered a natural antioxidant for practical breeding and production purposes, with an optimal concentration of crude extracts in this experiment being 50 μg/mL.
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spelling pubmed-101352522023-04-28 Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage Yun, Yanhong Shi, Huiyu Wang, Yanyu Yang, Fengyuan Zhang, Yuanxin Feng, Haibo Chen, Junpu Wang, Xuemei Animals (Basel) Article SIMPLE SUMMARY: The Dioscorea alata L. is known to contain abundant proanthocyanidins and anthocyanins, which exhibit potent antioxidant properties capable of scavenging free radicals. Additionally, these compounds have been shown to effectively promote the proliferation of IPEC-J2 cells within a pharmacotoxicity range. Conversely, exposure to H(2)O(2) can damage IPEC-J2 cells and trigger increased activity of cellular antioxidant enzymes. This study investigated the impact of crude extract from Dioscorea alata L. on the antioxidant capacity of IPEC-J2 cells and explored the pre-protective mechanism of Dioscorea alata L. against oxidative damage induced by H(2)O(2) through an examination of its effect on the NF-κB inflammatory signaling pathway. ABSTRACT: The purple tubers of Dioscorea alata L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of Dioscorea alata L. and its crude extracts on cells prior to oxidative stress. To establish a suitable oxidative damage model, an injured model of IPEC-J2 cells was created using H(2)O(2) as the oxidant. Specifically, when the concentration of H(2)O(2) was 120 μmol/L and the injured time was 8 h, the survival rate of cells decreased to approximately 70%, and the cells exhibited a noticeable oxidative stress reaction. Moreover, the crude extracts of Dioscorea alata L. demonstrated beneficial pre-protective effects on IPEC-J2 cells by increasing the total antioxidant capacity (T-AOC) and catalase (CAT) activities, augmenting the expression of total superoxide dismutase (T-SOD) and its genes, reducing the content of malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-P(X)) and its expression of genes, and promoting the expression of glucose transporter SGLT1 gene while reducing that of GULT2 gene, thereby facilitating the entry of anthocyanins into cells. In addition, the 50 μg/mL crude extracts effectively inhibited the phosphorylation of IκB and the p65 protein, thus reducing cellular oxidative stress. Given these findings, Dioscorea alata L. can be considered a natural antioxidant for practical breeding and production purposes, with an optimal concentration of crude extracts in this experiment being 50 μg/mL. MDPI 2023-04-19 /pmc/articles/PMC10135252/ /pubmed/37106964 http://dx.doi.org/10.3390/ani13081401 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yun, Yanhong
Shi, Huiyu
Wang, Yanyu
Yang, Fengyuan
Zhang, Yuanxin
Feng, Haibo
Chen, Junpu
Wang, Xuemei
Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage
title Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage
title_full Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage
title_fullStr Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage
title_full_unstemmed Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage
title_short Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage
title_sort pre-protection and mechanism of crude extracts from dioscorea alata l. on h(2)o(2)-induced ipec-j2 cells oxidative damage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135252/
https://www.ncbi.nlm.nih.gov/pubmed/37106964
http://dx.doi.org/10.3390/ani13081401
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