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Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells

SIMPLE SUMMARY: The aim of this study was to identify new and innovative strategies to improve the tissue-regeneration process. Concentrated growth factor (CGF) is an autologous biomaterial rich in growth factors and multipotent stem cells. The purpose of our study was to evaluate the osteogenic dif...

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Autores principales: Giannotti, Laura, Di Chiara Stanca, Benedetta, Nitti, Paola, Spedicato, Francesco, Damiano, Fabrizio, Demitri, Christian, Calabriso, Nadia, Carluccio, Maria Annunziata, Palermo, Andrea, Ferrante, Franco, Siculella, Luisa, Stanca, Eleonora
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135480/
https://www.ncbi.nlm.nih.gov/pubmed/37106729
http://dx.doi.org/10.3390/biology12040528
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author Giannotti, Laura
Di Chiara Stanca, Benedetta
Nitti, Paola
Spedicato, Francesco
Damiano, Fabrizio
Demitri, Christian
Calabriso, Nadia
Carluccio, Maria Annunziata
Palermo, Andrea
Ferrante, Franco
Siculella, Luisa
Stanca, Eleonora
author_facet Giannotti, Laura
Di Chiara Stanca, Benedetta
Nitti, Paola
Spedicato, Francesco
Damiano, Fabrizio
Demitri, Christian
Calabriso, Nadia
Carluccio, Maria Annunziata
Palermo, Andrea
Ferrante, Franco
Siculella, Luisa
Stanca, Eleonora
author_sort Giannotti, Laura
collection PubMed
description SIMPLE SUMMARY: The aim of this study was to identify new and innovative strategies to improve the tissue-regeneration process. Concentrated growth factor (CGF) is an autologous biomaterial rich in growth factors and multipotent stem cells. The purpose of our study was to evaluate the osteogenic differentiation of CGF primary cells in the presence of a hydroxyapatite–silicon scaffold, which represents a very interesting material in the field of bone reconstructive surgery. Our findings showed that the hydroxyapatite–silicon scaffold provided support to primary CGF cells by enhancing osteogenic differentiation. These data suggest interesting perspectives in the use of CGF together with scaffolds in the field of regenerative medicine. ABSTRACT: The application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells, was used together with a hydroxyapatite and silicon (HA-Si) scaffold, which represents a very interesting material in the field of bone reconstructive surgery. The aim of this work was to evaluate the potential osteogenic differentiation of CGF primary cells induced by HA-Si scaffolds. The cellular viability of CGF primary cells cultured on HA-Si scaffolds and their structural characterization were performed by MTT assay and SEM analysis, respectively. Moreover, the matrix mineralization of CGF primary cells on the HA-Si scaffold was evaluated through Alizarin red staining. The expression of osteogenic differentiation markers was investigated through mRNA quantification by real-time PCR. We found that the HA-Si scaffold was not cytotoxic for CGF primary cells, allowing their growth and proliferation. Furthermore, the HA-Si scaffold was able to induce increased levels of osteogenic markers, decreased levels of stemness markers in these cells, and the formation of a mineralized matrix. In conclusion, our results suggest that HA-Si scaffolds can be used as a biomaterial support for CGF application in the field of tissue regeneration.
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spelling pubmed-101354802023-04-28 Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells Giannotti, Laura Di Chiara Stanca, Benedetta Nitti, Paola Spedicato, Francesco Damiano, Fabrizio Demitri, Christian Calabriso, Nadia Carluccio, Maria Annunziata Palermo, Andrea Ferrante, Franco Siculella, Luisa Stanca, Eleonora Biology (Basel) Article SIMPLE SUMMARY: The aim of this study was to identify new and innovative strategies to improve the tissue-regeneration process. Concentrated growth factor (CGF) is an autologous biomaterial rich in growth factors and multipotent stem cells. The purpose of our study was to evaluate the osteogenic differentiation of CGF primary cells in the presence of a hydroxyapatite–silicon scaffold, which represents a very interesting material in the field of bone reconstructive surgery. Our findings showed that the hydroxyapatite–silicon scaffold provided support to primary CGF cells by enhancing osteogenic differentiation. These data suggest interesting perspectives in the use of CGF together with scaffolds in the field of regenerative medicine. ABSTRACT: The application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells, was used together with a hydroxyapatite and silicon (HA-Si) scaffold, which represents a very interesting material in the field of bone reconstructive surgery. The aim of this work was to evaluate the potential osteogenic differentiation of CGF primary cells induced by HA-Si scaffolds. The cellular viability of CGF primary cells cultured on HA-Si scaffolds and their structural characterization were performed by MTT assay and SEM analysis, respectively. Moreover, the matrix mineralization of CGF primary cells on the HA-Si scaffold was evaluated through Alizarin red staining. The expression of osteogenic differentiation markers was investigated through mRNA quantification by real-time PCR. We found that the HA-Si scaffold was not cytotoxic for CGF primary cells, allowing their growth and proliferation. Furthermore, the HA-Si scaffold was able to induce increased levels of osteogenic markers, decreased levels of stemness markers in these cells, and the formation of a mineralized matrix. In conclusion, our results suggest that HA-Si scaffolds can be used as a biomaterial support for CGF application in the field of tissue regeneration. MDPI 2023-03-30 /pmc/articles/PMC10135480/ /pubmed/37106729 http://dx.doi.org/10.3390/biology12040528 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giannotti, Laura
Di Chiara Stanca, Benedetta
Nitti, Paola
Spedicato, Francesco
Damiano, Fabrizio
Demitri, Christian
Calabriso, Nadia
Carluccio, Maria Annunziata
Palermo, Andrea
Ferrante, Franco
Siculella, Luisa
Stanca, Eleonora
Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells
title Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells
title_full Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells
title_fullStr Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells
title_full_unstemmed Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells
title_short Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells
title_sort hydroxyapatite–silicon scaffold promotes osteogenic differentiation of cgf primary cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135480/
https://www.ncbi.nlm.nih.gov/pubmed/37106729
http://dx.doi.org/10.3390/biology12040528
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