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Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field

SIMPLE SUMMARY: The objective of this study was to determine the effects of compost application on soil bacterial and fungal communities at the tillering, booting and ripening stages of barley plant growth. The two treatments were unfertilized (control) and compost treatment (30 t ha(−1)). The high-...

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Autores principales: Ghouili, Emna, Abid, Ghassen, Hogue, Richard, Jeanne, Thomas, D’Astous-Pagé, Joël, Sassi, Khaled, Hidri, Yassine, M’Hamed, Hatem Cheikh, Somenahally, Anil, Xue, Qingwu, Jebara, Moez, Nefissi Ouertani, Rim, Riahi, Jouhaina, de Oliveira, Ana Caroline, Muhovski, Yordan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135526/
https://www.ncbi.nlm.nih.gov/pubmed/37106747
http://dx.doi.org/10.3390/biology12040546
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author Ghouili, Emna
Abid, Ghassen
Hogue, Richard
Jeanne, Thomas
D’Astous-Pagé, Joël
Sassi, Khaled
Hidri, Yassine
M’Hamed, Hatem Cheikh
Somenahally, Anil
Xue, Qingwu
Jebara, Moez
Nefissi Ouertani, Rim
Riahi, Jouhaina
de Oliveira, Ana Caroline
Muhovski, Yordan
author_facet Ghouili, Emna
Abid, Ghassen
Hogue, Richard
Jeanne, Thomas
D’Astous-Pagé, Joël
Sassi, Khaled
Hidri, Yassine
M’Hamed, Hatem Cheikh
Somenahally, Anil
Xue, Qingwu
Jebara, Moez
Nefissi Ouertani, Rim
Riahi, Jouhaina
de Oliveira, Ana Caroline
Muhovski, Yordan
author_sort Ghouili, Emna
collection PubMed
description SIMPLE SUMMARY: The objective of this study was to determine the effects of compost application on soil bacterial and fungal communities at the tillering, booting and ripening stages of barley plant growth. The two treatments were unfertilized (control) and compost treatment (30 t ha(−1)). The high-throughput sequencing approach and real-time quantitative PCR method were performed to study the characteristics of soil bacterial and fungal communities. Compost addition significantly altered the compositions of bacterial and fungal communities. Our results revealed that the Chao1 index of the fungal community increased at the tillering and booting stage, while that of the bacterial community decreased at the tillering and ripening stage. Moreover, compost decreased the Shannon index of fungal and bacterial communities, especially at the tillering stage. The dominant bacterial phyla found in the samples were Proteobacteria and Actinobacteria while the dominant fungal phyla were Ascomycota and Mortierellomycota. Identified bacteria were mainly involved in energy metabolism, amino acid metabolism, metabolism of cofactors and vitamins and carbohydrate metabolism while fungi were saprotroph, pathotroph symbiotroph and endophyte. Overall, date palm waste compost addition could be considered as a sustainable practice for establishing a healthy soil microbiome and subsequently improving the soil quality and barley crop production. ABSTRACT: Application of date palm waste compost is quite beneficial in improving soil properties and crop growth. However, the effect of its application on soil microbial communities is less understood. High-throughput sequencing and quantitative real-time PCR (qPCR) were used to evaluate the effect of compost application on the soil microbial composition in a barley field during the tillering, booting and ripening stages. The results showed that compost treatment had the highest bacterial and fungal abundance, and its application significantly altered the richness (Chao1 index) and α-diversity (Shannon index) of fungal and bacterial communities. The dominant bacterial phyla found in the samples were Proteobacteria and Actinobacteria while the dominant fungal orders were Ascomycota and Mortierellomycota. Interestingly, compost enriched the relative abundance of beneficial microorganisms such as Chaetomium, Actinobacteriota, Talaromyces and Mortierella and reduced those of harmful microorganisms such as Alternaria, Aspergillus and Neocosmospora. Functional prediction based on Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that amplicon sequence variant (ASV) sequences related to energy metabolism, amino acid metabolism and carbohydrate metabolism were associated with compost-treated soil. Based on Fungi Functional Guild (FUNGuild), identified fungi community metabolic functions such as wood saprotroph, pathotroph, symbiotroph and endophyte were associated with compost-treated soil. Overall, compost addition could be considered as a sustainable practice for establishing a healthy soil microbiome and subsequently improving the soil quality and barley crop production.
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spelling pubmed-101355262023-04-28 Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field Ghouili, Emna Abid, Ghassen Hogue, Richard Jeanne, Thomas D’Astous-Pagé, Joël Sassi, Khaled Hidri, Yassine M’Hamed, Hatem Cheikh Somenahally, Anil Xue, Qingwu Jebara, Moez Nefissi Ouertani, Rim Riahi, Jouhaina de Oliveira, Ana Caroline Muhovski, Yordan Biology (Basel) Article SIMPLE SUMMARY: The objective of this study was to determine the effects of compost application on soil bacterial and fungal communities at the tillering, booting and ripening stages of barley plant growth. The two treatments were unfertilized (control) and compost treatment (30 t ha(−1)). The high-throughput sequencing approach and real-time quantitative PCR method were performed to study the characteristics of soil bacterial and fungal communities. Compost addition significantly altered the compositions of bacterial and fungal communities. Our results revealed that the Chao1 index of the fungal community increased at the tillering and booting stage, while that of the bacterial community decreased at the tillering and ripening stage. Moreover, compost decreased the Shannon index of fungal and bacterial communities, especially at the tillering stage. The dominant bacterial phyla found in the samples were Proteobacteria and Actinobacteria while the dominant fungal phyla were Ascomycota and Mortierellomycota. Identified bacteria were mainly involved in energy metabolism, amino acid metabolism, metabolism of cofactors and vitamins and carbohydrate metabolism while fungi were saprotroph, pathotroph symbiotroph and endophyte. Overall, date palm waste compost addition could be considered as a sustainable practice for establishing a healthy soil microbiome and subsequently improving the soil quality and barley crop production. ABSTRACT: Application of date palm waste compost is quite beneficial in improving soil properties and crop growth. However, the effect of its application on soil microbial communities is less understood. High-throughput sequencing and quantitative real-time PCR (qPCR) were used to evaluate the effect of compost application on the soil microbial composition in a barley field during the tillering, booting and ripening stages. The results showed that compost treatment had the highest bacterial and fungal abundance, and its application significantly altered the richness (Chao1 index) and α-diversity (Shannon index) of fungal and bacterial communities. The dominant bacterial phyla found in the samples were Proteobacteria and Actinobacteria while the dominant fungal orders were Ascomycota and Mortierellomycota. Interestingly, compost enriched the relative abundance of beneficial microorganisms such as Chaetomium, Actinobacteriota, Talaromyces and Mortierella and reduced those of harmful microorganisms such as Alternaria, Aspergillus and Neocosmospora. Functional prediction based on Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that amplicon sequence variant (ASV) sequences related to energy metabolism, amino acid metabolism and carbohydrate metabolism were associated with compost-treated soil. Based on Fungi Functional Guild (FUNGuild), identified fungi community metabolic functions such as wood saprotroph, pathotroph, symbiotroph and endophyte were associated with compost-treated soil. Overall, compost addition could be considered as a sustainable practice for establishing a healthy soil microbiome and subsequently improving the soil quality and barley crop production. MDPI 2023-04-03 /pmc/articles/PMC10135526/ /pubmed/37106747 http://dx.doi.org/10.3390/biology12040546 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghouili, Emna
Abid, Ghassen
Hogue, Richard
Jeanne, Thomas
D’Astous-Pagé, Joël
Sassi, Khaled
Hidri, Yassine
M’Hamed, Hatem Cheikh
Somenahally, Anil
Xue, Qingwu
Jebara, Moez
Nefissi Ouertani, Rim
Riahi, Jouhaina
de Oliveira, Ana Caroline
Muhovski, Yordan
Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field
title Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field
title_full Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field
title_fullStr Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field
title_full_unstemmed Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field
title_short Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field
title_sort date palm waste compost application increases soil microbial community diversity in a cropping barley (hordeum vulgare l.) field
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135526/
https://www.ncbi.nlm.nih.gov/pubmed/37106747
http://dx.doi.org/10.3390/biology12040546
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