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Synthesis of Sulfides and Persulfides Is Not Impeded by Disruption of Three Canonical Enzymes in Sulfur Metabolism

Reactive sulfur species, or persulfides and polysulfides, such as cysteine hydropersulfide and glutathione persulfide, are endogenously produced in abundance in both prokaryotes and eukaryotes, including mammals. Various forms of reactive persulfides occur in both low-molecular-weight and protein-bo...

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Autores principales: Zainol Abidin, Qamarul Hafiz, Ida, Tomoaki, Morita, Masanobu, Matsunaga, Tetsuro, Nishimura, Akira, Jung, Minkyung, Hassan, Naim, Takata, Tsuyoshi, Ishii, Isao, Kruger, Warren, Wang, Rui, Motohashi, Hozumi, Tsutsui, Masato, Akaike, Takaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135671/
https://www.ncbi.nlm.nih.gov/pubmed/37107243
http://dx.doi.org/10.3390/antiox12040868
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author Zainol Abidin, Qamarul Hafiz
Ida, Tomoaki
Morita, Masanobu
Matsunaga, Tetsuro
Nishimura, Akira
Jung, Minkyung
Hassan, Naim
Takata, Tsuyoshi
Ishii, Isao
Kruger, Warren
Wang, Rui
Motohashi, Hozumi
Tsutsui, Masato
Akaike, Takaaki
author_facet Zainol Abidin, Qamarul Hafiz
Ida, Tomoaki
Morita, Masanobu
Matsunaga, Tetsuro
Nishimura, Akira
Jung, Minkyung
Hassan, Naim
Takata, Tsuyoshi
Ishii, Isao
Kruger, Warren
Wang, Rui
Motohashi, Hozumi
Tsutsui, Masato
Akaike, Takaaki
author_sort Zainol Abidin, Qamarul Hafiz
collection PubMed
description Reactive sulfur species, or persulfides and polysulfides, such as cysteine hydropersulfide and glutathione persulfide, are endogenously produced in abundance in both prokaryotes and eukaryotes, including mammals. Various forms of reactive persulfides occur in both low-molecular-weight and protein-bound thiols. The chemical properties and great supply of these molecular species suggest a pivotal role for reactive persulfides/polysulfides in different cellular regulatory processes (e.g., energy metabolism and redox signaling). We demonstrated earlier that cysteinyl-tRNA synthetase (CARS) is a new cysteine persulfide synthase (CPERS) and is responsible for the in vivo production of most reactive persulfides (polysulfides). Some researchers continue to suggest that 3-mercaptopyruvate sulfurtransferase (3-MST), cystathionine β-synthase (CBS), and cystathionine γ-lyase (CSE) may also produce hydrogen sulfide and persulfides that may be generated during the transfer of sulfur from 3-mercaptopyruvate to the cysteine residues of 3-MST or direct synthesis from cysteine by CBS/CSE, respectively. We thus used integrated sulfur metabolome analysis, which we recently developed, with 3-MST knockout (KO) mice and CBS/CSE/3-MST triple-KO mice, to elucidate the possible contribution of 3-MST, CBS, and CSE to the production of reactive persulfides in vivo. We therefore quantified various sulfide metabolites in organs derived from these mutant mice and their wild-type littermates via this sulfur metabolome, which clearly revealed no significant difference between mutant mice and wild-type mice in terms of reactive persulfide production. This result indicates that 3-MST, CBS, and CSE are not major sources of endogenous reactive persulfide production; rather, CARS/CPERS is the principal enzyme that is actually involved in and even primarily responsible for the biosynthesis of reactive persulfides and polysulfides in vivo in mammals.
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spelling pubmed-101356712023-04-28 Synthesis of Sulfides and Persulfides Is Not Impeded by Disruption of Three Canonical Enzymes in Sulfur Metabolism Zainol Abidin, Qamarul Hafiz Ida, Tomoaki Morita, Masanobu Matsunaga, Tetsuro Nishimura, Akira Jung, Minkyung Hassan, Naim Takata, Tsuyoshi Ishii, Isao Kruger, Warren Wang, Rui Motohashi, Hozumi Tsutsui, Masato Akaike, Takaaki Antioxidants (Basel) Article Reactive sulfur species, or persulfides and polysulfides, such as cysteine hydropersulfide and glutathione persulfide, are endogenously produced in abundance in both prokaryotes and eukaryotes, including mammals. Various forms of reactive persulfides occur in both low-molecular-weight and protein-bound thiols. The chemical properties and great supply of these molecular species suggest a pivotal role for reactive persulfides/polysulfides in different cellular regulatory processes (e.g., energy metabolism and redox signaling). We demonstrated earlier that cysteinyl-tRNA synthetase (CARS) is a new cysteine persulfide synthase (CPERS) and is responsible for the in vivo production of most reactive persulfides (polysulfides). Some researchers continue to suggest that 3-mercaptopyruvate sulfurtransferase (3-MST), cystathionine β-synthase (CBS), and cystathionine γ-lyase (CSE) may also produce hydrogen sulfide and persulfides that may be generated during the transfer of sulfur from 3-mercaptopyruvate to the cysteine residues of 3-MST or direct synthesis from cysteine by CBS/CSE, respectively. We thus used integrated sulfur metabolome analysis, which we recently developed, with 3-MST knockout (KO) mice and CBS/CSE/3-MST triple-KO mice, to elucidate the possible contribution of 3-MST, CBS, and CSE to the production of reactive persulfides in vivo. We therefore quantified various sulfide metabolites in organs derived from these mutant mice and their wild-type littermates via this sulfur metabolome, which clearly revealed no significant difference between mutant mice and wild-type mice in terms of reactive persulfide production. This result indicates that 3-MST, CBS, and CSE are not major sources of endogenous reactive persulfide production; rather, CARS/CPERS is the principal enzyme that is actually involved in and even primarily responsible for the biosynthesis of reactive persulfides and polysulfides in vivo in mammals. MDPI 2023-04-03 /pmc/articles/PMC10135671/ /pubmed/37107243 http://dx.doi.org/10.3390/antiox12040868 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zainol Abidin, Qamarul Hafiz
Ida, Tomoaki
Morita, Masanobu
Matsunaga, Tetsuro
Nishimura, Akira
Jung, Minkyung
Hassan, Naim
Takata, Tsuyoshi
Ishii, Isao
Kruger, Warren
Wang, Rui
Motohashi, Hozumi
Tsutsui, Masato
Akaike, Takaaki
Synthesis of Sulfides and Persulfides Is Not Impeded by Disruption of Three Canonical Enzymes in Sulfur Metabolism
title Synthesis of Sulfides and Persulfides Is Not Impeded by Disruption of Three Canonical Enzymes in Sulfur Metabolism
title_full Synthesis of Sulfides and Persulfides Is Not Impeded by Disruption of Three Canonical Enzymes in Sulfur Metabolism
title_fullStr Synthesis of Sulfides and Persulfides Is Not Impeded by Disruption of Three Canonical Enzymes in Sulfur Metabolism
title_full_unstemmed Synthesis of Sulfides and Persulfides Is Not Impeded by Disruption of Three Canonical Enzymes in Sulfur Metabolism
title_short Synthesis of Sulfides and Persulfides Is Not Impeded by Disruption of Three Canonical Enzymes in Sulfur Metabolism
title_sort synthesis of sulfides and persulfides is not impeded by disruption of three canonical enzymes in sulfur metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135671/
https://www.ncbi.nlm.nih.gov/pubmed/37107243
http://dx.doi.org/10.3390/antiox12040868
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