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The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression
Type 1 diabetes mellitus (T1DM) is one of the major chronic diseases in children worldwide. This study aimed to investigate interleukin-10 (IL-10) gene expression and tumor necrosis factor-alpha (TNF-α) in T1DM. A total of 107 patients were included, 15 were T1DM in ketoacidosis, 30 patients had T1D...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135733/ https://www.ncbi.nlm.nih.gov/pubmed/37189738 http://dx.doi.org/10.3390/biomedicines11041120 |
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author | dos Santos Haber, Jesselina Francisco Barbalho, Sandra Maria Sgarbi, Jose Augusto de Argollo Haber, Rafael Santos de Labio, Roger William Laurindo, Lucas Fornari Chagas, Eduardo Federighi Baisi Payão, Spencer Luiz Marques |
author_facet | dos Santos Haber, Jesselina Francisco Barbalho, Sandra Maria Sgarbi, Jose Augusto de Argollo Haber, Rafael Santos de Labio, Roger William Laurindo, Lucas Fornari Chagas, Eduardo Federighi Baisi Payão, Spencer Luiz Marques |
author_sort | dos Santos Haber, Jesselina Francisco |
collection | PubMed |
description | Type 1 diabetes mellitus (T1DM) is one of the major chronic diseases in children worldwide. This study aimed to investigate interleukin-10 (IL-10) gene expression and tumor necrosis factor-alpha (TNF-α) in T1DM. A total of 107 patients were included, 15 were T1DM in ketoacidosis, 30 patients had T1DM and HbA1c ≥ 8%; 32 patients had T1DM and presented HbA1c < 8%; and 30 were controls. The expression of peripheral blood mononuclear cells was performed using the reverse transcriptase–polymerase chain reaction in real time. The cytokines gene expression was higher in patients with T1DM. The IL-10 gene expression increased substantially in patients with ketoacidosis, and there was a positive correlation with HbA1c. A negative correlation was found for IL-10 expression and the age of patients with diabetes, and the time of diagnosis of the disease. There was a positive correlation between TNF-α expression with age. The expression of IL-10 and TNF-α genes showed a significant increase in DM1 patients. Once current T1DM treatment is based on exogenous insulin, there is a need for other therapies, and inflammatory biomarkers could bring new possibilities to the therapeutic approach of the patients. |
format | Online Article Text |
id | pubmed-10135733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101357332023-04-28 The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression dos Santos Haber, Jesselina Francisco Barbalho, Sandra Maria Sgarbi, Jose Augusto de Argollo Haber, Rafael Santos de Labio, Roger William Laurindo, Lucas Fornari Chagas, Eduardo Federighi Baisi Payão, Spencer Luiz Marques Biomedicines Article Type 1 diabetes mellitus (T1DM) is one of the major chronic diseases in children worldwide. This study aimed to investigate interleukin-10 (IL-10) gene expression and tumor necrosis factor-alpha (TNF-α) in T1DM. A total of 107 patients were included, 15 were T1DM in ketoacidosis, 30 patients had T1DM and HbA1c ≥ 8%; 32 patients had T1DM and presented HbA1c < 8%; and 30 were controls. The expression of peripheral blood mononuclear cells was performed using the reverse transcriptase–polymerase chain reaction in real time. The cytokines gene expression was higher in patients with T1DM. The IL-10 gene expression increased substantially in patients with ketoacidosis, and there was a positive correlation with HbA1c. A negative correlation was found for IL-10 expression and the age of patients with diabetes, and the time of diagnosis of the disease. There was a positive correlation between TNF-α expression with age. The expression of IL-10 and TNF-α genes showed a significant increase in DM1 patients. Once current T1DM treatment is based on exogenous insulin, there is a need for other therapies, and inflammatory biomarkers could bring new possibilities to the therapeutic approach of the patients. MDPI 2023-04-07 /pmc/articles/PMC10135733/ /pubmed/37189738 http://dx.doi.org/10.3390/biomedicines11041120 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article dos Santos Haber, Jesselina Francisco Barbalho, Sandra Maria Sgarbi, Jose Augusto de Argollo Haber, Rafael Santos de Labio, Roger William Laurindo, Lucas Fornari Chagas, Eduardo Federighi Baisi Payão, Spencer Luiz Marques The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression |
title | The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression |
title_full | The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression |
title_fullStr | The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression |
title_full_unstemmed | The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression |
title_short | The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression |
title_sort | relationship between type 1 diabetes mellitus, tnf-α, and il-10 gene expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135733/ https://www.ncbi.nlm.nih.gov/pubmed/37189738 http://dx.doi.org/10.3390/biomedicines11041120 |
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