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The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression

Type 1 diabetes mellitus (T1DM) is one of the major chronic diseases in children worldwide. This study aimed to investigate interleukin-10 (IL-10) gene expression and tumor necrosis factor-alpha (TNF-α) in T1DM. A total of 107 patients were included, 15 were T1DM in ketoacidosis, 30 patients had T1D...

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Autores principales: dos Santos Haber, Jesselina Francisco, Barbalho, Sandra Maria, Sgarbi, Jose Augusto, de Argollo Haber, Rafael Santos, de Labio, Roger William, Laurindo, Lucas Fornari, Chagas, Eduardo Federighi Baisi, Payão, Spencer Luiz Marques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135733/
https://www.ncbi.nlm.nih.gov/pubmed/37189738
http://dx.doi.org/10.3390/biomedicines11041120
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author dos Santos Haber, Jesselina Francisco
Barbalho, Sandra Maria
Sgarbi, Jose Augusto
de Argollo Haber, Rafael Santos
de Labio, Roger William
Laurindo, Lucas Fornari
Chagas, Eduardo Federighi Baisi
Payão, Spencer Luiz Marques
author_facet dos Santos Haber, Jesselina Francisco
Barbalho, Sandra Maria
Sgarbi, Jose Augusto
de Argollo Haber, Rafael Santos
de Labio, Roger William
Laurindo, Lucas Fornari
Chagas, Eduardo Federighi Baisi
Payão, Spencer Luiz Marques
author_sort dos Santos Haber, Jesselina Francisco
collection PubMed
description Type 1 diabetes mellitus (T1DM) is one of the major chronic diseases in children worldwide. This study aimed to investigate interleukin-10 (IL-10) gene expression and tumor necrosis factor-alpha (TNF-α) in T1DM. A total of 107 patients were included, 15 were T1DM in ketoacidosis, 30 patients had T1DM and HbA1c ≥ 8%; 32 patients had T1DM and presented HbA1c < 8%; and 30 were controls. The expression of peripheral blood mononuclear cells was performed using the reverse transcriptase–polymerase chain reaction in real time. The cytokines gene expression was higher in patients with T1DM. The IL-10 gene expression increased substantially in patients with ketoacidosis, and there was a positive correlation with HbA1c. A negative correlation was found for IL-10 expression and the age of patients with diabetes, and the time of diagnosis of the disease. There was a positive correlation between TNF-α expression with age. The expression of IL-10 and TNF-α genes showed a significant increase in DM1 patients. Once current T1DM treatment is based on exogenous insulin, there is a need for other therapies, and inflammatory biomarkers could bring new possibilities to the therapeutic approach of the patients.
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spelling pubmed-101357332023-04-28 The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression dos Santos Haber, Jesselina Francisco Barbalho, Sandra Maria Sgarbi, Jose Augusto de Argollo Haber, Rafael Santos de Labio, Roger William Laurindo, Lucas Fornari Chagas, Eduardo Federighi Baisi Payão, Spencer Luiz Marques Biomedicines Article Type 1 diabetes mellitus (T1DM) is one of the major chronic diseases in children worldwide. This study aimed to investigate interleukin-10 (IL-10) gene expression and tumor necrosis factor-alpha (TNF-α) in T1DM. A total of 107 patients were included, 15 were T1DM in ketoacidosis, 30 patients had T1DM and HbA1c ≥ 8%; 32 patients had T1DM and presented HbA1c < 8%; and 30 were controls. The expression of peripheral blood mononuclear cells was performed using the reverse transcriptase–polymerase chain reaction in real time. The cytokines gene expression was higher in patients with T1DM. The IL-10 gene expression increased substantially in patients with ketoacidosis, and there was a positive correlation with HbA1c. A negative correlation was found for IL-10 expression and the age of patients with diabetes, and the time of diagnosis of the disease. There was a positive correlation between TNF-α expression with age. The expression of IL-10 and TNF-α genes showed a significant increase in DM1 patients. Once current T1DM treatment is based on exogenous insulin, there is a need for other therapies, and inflammatory biomarkers could bring new possibilities to the therapeutic approach of the patients. MDPI 2023-04-07 /pmc/articles/PMC10135733/ /pubmed/37189738 http://dx.doi.org/10.3390/biomedicines11041120 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
dos Santos Haber, Jesselina Francisco
Barbalho, Sandra Maria
Sgarbi, Jose Augusto
de Argollo Haber, Rafael Santos
de Labio, Roger William
Laurindo, Lucas Fornari
Chagas, Eduardo Federighi Baisi
Payão, Spencer Luiz Marques
The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression
title The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression
title_full The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression
title_fullStr The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression
title_full_unstemmed The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression
title_short The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression
title_sort relationship between type 1 diabetes mellitus, tnf-α, and il-10 gene expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135733/
https://www.ncbi.nlm.nih.gov/pubmed/37189738
http://dx.doi.org/10.3390/biomedicines11041120
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