Cargando…

New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation

This study introduces a new generation of dielectrophoretic-based microfluidic device for the precise separation of multiple particle/cell types. The device features two sets of 3D electrodes, namely cylindrical and sidewall electrodes. The main channel of the device terminates with three outlets: o...

Descripción completa

Detalles Bibliográficos
Autores principales: Sharbati, Pouya, Sadaghiani, Abdolali K., Koşar, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135750/
https://www.ncbi.nlm.nih.gov/pubmed/37185493
http://dx.doi.org/10.3390/bios13040418
_version_ 1785032053870297088
author Sharbati, Pouya
Sadaghiani, Abdolali K.
Koşar, Ali
author_facet Sharbati, Pouya
Sadaghiani, Abdolali K.
Koşar, Ali
author_sort Sharbati, Pouya
collection PubMed
description This study introduces a new generation of dielectrophoretic-based microfluidic device for the precise separation of multiple particle/cell types. The device features two sets of 3D electrodes, namely cylindrical and sidewall electrodes. The main channel of the device terminates with three outlets: one in the middle for particles that sense negative dielectrophoresis force and two others at the right and left sides for particles that sense positive dielectrophoresis force. To evaluate the device performance, we used red blood cells (RBCs), T-cells, U937-MC cells, and Clostridium difficile bacteria as our test subjects. Our results demonstrate that the proposed microfluidic device could accurately separate bioparticles in two steps, with sidewall electrodes of 200 µm proving optimal for efficient separation. Applying different voltages for each separation step, we found that the device performed most effectively at 6 Vp-p applied to the 3D electrodes, and at 20 Vp-p and 11 Vp-p applied to the sidewall electrodes for separating RBCs from bacteria and T-cells from U937-MC cells, respectively. Notably, the device’s maximum electric fields remained below the cell electroporation threshold, and we achieved a separation efficiency of 95.5% for multi-type particle separation. Our findings proved the device’s capacity for separating multiple particle types with high accuracy, without limitation for particle variety.
format Online
Article
Text
id pubmed-10135750
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101357502023-04-28 New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation Sharbati, Pouya Sadaghiani, Abdolali K. Koşar, Ali Biosensors (Basel) Article This study introduces a new generation of dielectrophoretic-based microfluidic device for the precise separation of multiple particle/cell types. The device features two sets of 3D electrodes, namely cylindrical and sidewall electrodes. The main channel of the device terminates with three outlets: one in the middle for particles that sense negative dielectrophoresis force and two others at the right and left sides for particles that sense positive dielectrophoresis force. To evaluate the device performance, we used red blood cells (RBCs), T-cells, U937-MC cells, and Clostridium difficile bacteria as our test subjects. Our results demonstrate that the proposed microfluidic device could accurately separate bioparticles in two steps, with sidewall electrodes of 200 µm proving optimal for efficient separation. Applying different voltages for each separation step, we found that the device performed most effectively at 6 Vp-p applied to the 3D electrodes, and at 20 Vp-p and 11 Vp-p applied to the sidewall electrodes for separating RBCs from bacteria and T-cells from U937-MC cells, respectively. Notably, the device’s maximum electric fields remained below the cell electroporation threshold, and we achieved a separation efficiency of 95.5% for multi-type particle separation. Our findings proved the device’s capacity for separating multiple particle types with high accuracy, without limitation for particle variety. MDPI 2023-03-24 /pmc/articles/PMC10135750/ /pubmed/37185493 http://dx.doi.org/10.3390/bios13040418 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sharbati, Pouya
Sadaghiani, Abdolali K.
Koşar, Ali
New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation
title New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation
title_full New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation
title_fullStr New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation
title_full_unstemmed New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation
title_short New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation
title_sort new generation dielectrophoretic-based microfluidic device for multi-type cell separation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135750/
https://www.ncbi.nlm.nih.gov/pubmed/37185493
http://dx.doi.org/10.3390/bios13040418
work_keys_str_mv AT sharbatipouya newgenerationdielectrophoreticbasedmicrofluidicdeviceformultitypecellseparation
AT sadaghianiabdolalik newgenerationdielectrophoreticbasedmicrofluidicdeviceformultitypecellseparation
AT kosarali newgenerationdielectrophoreticbasedmicrofluidicdeviceformultitypecellseparation