Cargando…

Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution

HEK293 is a widely used cell line in the fields of research and industry. It is assumed that these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle image velocimetry validated computational fluid dynamics (CFD) to determine the hydrodynamic stress in both shak...

Descripción completa

Detalles Bibliográficos
Autores principales: Seidel, Stefan, Maschke, Rüdiger W., Mozaffari, Fruhar, Eibl-Schindler, Regine, Eibl, Dieter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135925/
https://www.ncbi.nlm.nih.gov/pubmed/37106665
http://dx.doi.org/10.3390/bioengineering10040478
_version_ 1785032096909099008
author Seidel, Stefan
Maschke, Rüdiger W.
Mozaffari, Fruhar
Eibl-Schindler, Regine
Eibl, Dieter
author_facet Seidel, Stefan
Maschke, Rüdiger W.
Mozaffari, Fruhar
Eibl-Schindler, Regine
Eibl, Dieter
author_sort Seidel, Stefan
collection PubMed
description HEK293 is a widely used cell line in the fields of research and industry. It is assumed that these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle image velocimetry validated computational fluid dynamics (CFD) to determine the hydrodynamic stress in both shake flasks, with and without baffles, and in stirred Minifors 2 bioreactors to evaluate its effect on the growth and aggregate size distribution of HEK293 suspension cells. The HEK FreeStyle(TM) 293-F cell line was cultivated in batch mode at different specific power inputs (from 63 [Formula: see text] to 451 [Formula: see text]), whereby [Formula: see text] corresponds to the upper limit, which is what has been typically described in published experiments. In addition to the specific growth rate and maximum viable cell density VCD [Formula: see text] , the cell size distribution over time and cluster size distribution were investigated. The VCD [Formula: see text] of [Formula: see text] [Formula: see text] [Formula: see text] [Formula: see text] was reached at a specific power input of 233 [Formula: see text] and was [Formula: see text] higher than the value obtained at 63 [Formula: see text] and [Formula: see text] higher than the value obtained at 451 [Formula: see text]. No significant change in the cell size distribution could be measured in the investigated range. It was shown that the cell cluster size distribution follows a strict geometric distribution whose free parameter p is linearly dependent on the mean Kolmogorov length scale. Based on the performed experiments, it has been shown that by using CFD-characterised bioreactors, the VCD [Formula: see text] can be increased and the cell aggregate rate can be precisely controlled.
format Online
Article
Text
id pubmed-10135925
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101359252023-04-28 Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution Seidel, Stefan Maschke, Rüdiger W. Mozaffari, Fruhar Eibl-Schindler, Regine Eibl, Dieter Bioengineering (Basel) Article HEK293 is a widely used cell line in the fields of research and industry. It is assumed that these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle image velocimetry validated computational fluid dynamics (CFD) to determine the hydrodynamic stress in both shake flasks, with and without baffles, and in stirred Minifors 2 bioreactors to evaluate its effect on the growth and aggregate size distribution of HEK293 suspension cells. The HEK FreeStyle(TM) 293-F cell line was cultivated in batch mode at different specific power inputs (from 63 [Formula: see text] to 451 [Formula: see text]), whereby [Formula: see text] corresponds to the upper limit, which is what has been typically described in published experiments. In addition to the specific growth rate and maximum viable cell density VCD [Formula: see text] , the cell size distribution over time and cluster size distribution were investigated. The VCD [Formula: see text] of [Formula: see text] [Formula: see text] [Formula: see text] [Formula: see text] was reached at a specific power input of 233 [Formula: see text] and was [Formula: see text] higher than the value obtained at 63 [Formula: see text] and [Formula: see text] higher than the value obtained at 451 [Formula: see text]. No significant change in the cell size distribution could be measured in the investigated range. It was shown that the cell cluster size distribution follows a strict geometric distribution whose free parameter p is linearly dependent on the mean Kolmogorov length scale. Based on the performed experiments, it has been shown that by using CFD-characterised bioreactors, the VCD [Formula: see text] can be increased and the cell aggregate rate can be precisely controlled. MDPI 2023-04-16 /pmc/articles/PMC10135925/ /pubmed/37106665 http://dx.doi.org/10.3390/bioengineering10040478 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seidel, Stefan
Maschke, Rüdiger W.
Mozaffari, Fruhar
Eibl-Schindler, Regine
Eibl, Dieter
Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution
title Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution
title_full Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution
title_fullStr Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution
title_full_unstemmed Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution
title_short Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution
title_sort improvement of hek293 cell growth by adapting hydrodynamic stress and predicting cell aggregate size distribution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135925/
https://www.ncbi.nlm.nih.gov/pubmed/37106665
http://dx.doi.org/10.3390/bioengineering10040478
work_keys_str_mv AT seidelstefan improvementofhek293cellgrowthbyadaptinghydrodynamicstressandpredictingcellaggregatesizedistribution
AT maschkerudigerw improvementofhek293cellgrowthbyadaptinghydrodynamicstressandpredictingcellaggregatesizedistribution
AT mozaffarifruhar improvementofhek293cellgrowthbyadaptinghydrodynamicstressandpredictingcellaggregatesizedistribution
AT eiblschindlerregine improvementofhek293cellgrowthbyadaptinghydrodynamicstressandpredictingcellaggregatesizedistribution
AT eibldieter improvementofhek293cellgrowthbyadaptinghydrodynamicstressandpredictingcellaggregatesizedistribution