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Biomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safety

The potential applications for cold plasma in medicine are extensive, from microbial inactivation and induction of apoptosis in cancer cells to stimulating wound healing and enhancing the blood coagulation cascade. The safe bio-medical application of cold plasma and subsequent effect on complex biol...

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Autores principales: Heslin, Caitlin, Boehm, Daniela, Gilmore, Brendan F., Megaw, Julianne, Freeman, Theresa A., Hickok, Noreen J., Cullen, P. J., Bourke, Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136044/
https://www.ncbi.nlm.nih.gov/pubmed/37124146
http://dx.doi.org/10.3389/fphy.2020.613046
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author Heslin, Caitlin
Boehm, Daniela
Gilmore, Brendan F.
Megaw, Julianne
Freeman, Theresa A.
Hickok, Noreen J.
Cullen, P. J.
Bourke, Paula
author_facet Heslin, Caitlin
Boehm, Daniela
Gilmore, Brendan F.
Megaw, Julianne
Freeman, Theresa A.
Hickok, Noreen J.
Cullen, P. J.
Bourke, Paula
author_sort Heslin, Caitlin
collection PubMed
description The potential applications for cold plasma in medicine are extensive, from microbial inactivation and induction of apoptosis in cancer cells to stimulating wound healing and enhancing the blood coagulation cascade. The safe bio-medical application of cold plasma and subsequent effect on complex biological pathways requires precision and a distinct understanding of how physiological redox chemistry is manipulated. Chemical modification of biomolecules such as carbohydrates, proteins, and lipids treated with cold plasma have been characterized, however, the context of how alterations of these molecules affect cell behavior or in vivo functionality has not been determined. Thus, this study examines the cytotoxic and mutagenic effects of plasma-treated molecules in vitro using CHO-K1 cells and in vivo in Galleria mellonella larvae. Specifically, albumin, glucose, cholesterol, and arachidonic acid were chosen as representative biomolecules, with established involvement in diverse bioprocesses including; cellular respiration, intracellular transport, cell signaling or membrane structure. Long- and short-term effects depended strongly on the molecule type and the treatment milieu indicating the impact of chemical and physical modifications on downstream biological pathways. Importantly, absence of short-term toxicity did not always correlate with absence of longer-term effects, indicating the need to comprehensively assess ongoing effects for diverse biological applications.
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spelling pubmed-101360442023-04-27 Biomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safety Heslin, Caitlin Boehm, Daniela Gilmore, Brendan F. Megaw, Julianne Freeman, Theresa A. Hickok, Noreen J. Cullen, P. J. Bourke, Paula Front Phys Article The potential applications for cold plasma in medicine are extensive, from microbial inactivation and induction of apoptosis in cancer cells to stimulating wound healing and enhancing the blood coagulation cascade. The safe bio-medical application of cold plasma and subsequent effect on complex biological pathways requires precision and a distinct understanding of how physiological redox chemistry is manipulated. Chemical modification of biomolecules such as carbohydrates, proteins, and lipids treated with cold plasma have been characterized, however, the context of how alterations of these molecules affect cell behavior or in vivo functionality has not been determined. Thus, this study examines the cytotoxic and mutagenic effects of plasma-treated molecules in vitro using CHO-K1 cells and in vivo in Galleria mellonella larvae. Specifically, albumin, glucose, cholesterol, and arachidonic acid were chosen as representative biomolecules, with established involvement in diverse bioprocesses including; cellular respiration, intracellular transport, cell signaling or membrane structure. Long- and short-term effects depended strongly on the molecule type and the treatment milieu indicating the impact of chemical and physical modifications on downstream biological pathways. Importantly, absence of short-term toxicity did not always correlate with absence of longer-term effects, indicating the need to comprehensively assess ongoing effects for diverse biological applications. 2021-01 2021-01-14 /pmc/articles/PMC10136044/ /pubmed/37124146 http://dx.doi.org/10.3389/fphy.2020.613046 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Article
Heslin, Caitlin
Boehm, Daniela
Gilmore, Brendan F.
Megaw, Julianne
Freeman, Theresa A.
Hickok, Noreen J.
Cullen, P. J.
Bourke, Paula
Biomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safety
title Biomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safety
title_full Biomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safety
title_fullStr Biomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safety
title_full_unstemmed Biomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safety
title_short Biomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safety
title_sort biomolecules as model indicators of in vitro and in vivo cold plasma safety
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136044/
https://www.ncbi.nlm.nih.gov/pubmed/37124146
http://dx.doi.org/10.3389/fphy.2020.613046
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