Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells

In this work, a coumarin derivative, SWJT-14, was synthesized as a fluorescence probe to distinguish cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in aqueous solutions. The detection limit of Cys, Hcy and GSH for the probe was 0.02 μM, 0.42 μM and 0.92 μM, respectively, which was lower th...

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Detalles Bibliográficos
Autores principales: Du, Wei, Gong, Xiu-Lin, Tian, Yang, Zhu, Xi, Peng, Yu, Wang, Ya-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136212/
https://www.ncbi.nlm.nih.gov/pubmed/37185522
http://dx.doi.org/10.3390/bios13040447
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author Du, Wei
Gong, Xiu-Lin
Tian, Yang
Zhu, Xi
Peng, Yu
Wang, Ya-Wen
author_facet Du, Wei
Gong, Xiu-Lin
Tian, Yang
Zhu, Xi
Peng, Yu
Wang, Ya-Wen
author_sort Du, Wei
collection PubMed
description In this work, a coumarin derivative, SWJT-14, was synthesized as a fluorescence probe to distinguish cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in aqueous solutions. The detection limit of Cys, Hcy and GSH for the probe was 0.02 μM, 0.42 μM and 0.92 μM, respectively, which was lower than biothiols in cells. The probe reacted with biothiols to generate different products with different conjugated structures. Additionally, it could distinguish Cys, Hcy and GSH using fluorescence and UV-Vis spectra. The detection mechanism was confirmed by MS. SWJT-14 was successfully used in cellular experiments and detected both endogenous and exogenous biothiols.
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spelling pubmed-101362122023-04-28 Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells Du, Wei Gong, Xiu-Lin Tian, Yang Zhu, Xi Peng, Yu Wang, Ya-Wen Biosensors (Basel) Communication In this work, a coumarin derivative, SWJT-14, was synthesized as a fluorescence probe to distinguish cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in aqueous solutions. The detection limit of Cys, Hcy and GSH for the probe was 0.02 μM, 0.42 μM and 0.92 μM, respectively, which was lower than biothiols in cells. The probe reacted with biothiols to generate different products with different conjugated structures. Additionally, it could distinguish Cys, Hcy and GSH using fluorescence and UV-Vis spectra. The detection mechanism was confirmed by MS. SWJT-14 was successfully used in cellular experiments and detected both endogenous and exogenous biothiols. MDPI 2023-03-31 /pmc/articles/PMC10136212/ /pubmed/37185522 http://dx.doi.org/10.3390/bios13040447 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Du, Wei
Gong, Xiu-Lin
Tian, Yang
Zhu, Xi
Peng, Yu
Wang, Ya-Wen
Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells
title Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells
title_full Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells
title_fullStr Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells
title_full_unstemmed Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells
title_short Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells
title_sort coumarin-based fluorescence probe for differentiated detection of biothiols and its bioimaging in cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136212/
https://www.ncbi.nlm.nih.gov/pubmed/37185522
http://dx.doi.org/10.3390/bios13040447
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