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Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients

Intrauterine growth restriction (IUGR) and preeclampsia (PE) are placental pathologies known to complicate pregnancy and cause neonatal disorders. To date, there is a limited number of studies on the genetic similarity of these conditions. DNA methylation is a heritable epigenetic process that can r...

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Autores principales: Norton, Carter, Clarke, Derek, Holmstrom, Joshua, Stirland, Isaac, Reynolds, Paul R., Jenkins, Tim G., Arroyo, Juan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136447/
https://www.ncbi.nlm.nih.gov/pubmed/37190039
http://dx.doi.org/10.3390/cells12081130
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author Norton, Carter
Clarke, Derek
Holmstrom, Joshua
Stirland, Isaac
Reynolds, Paul R.
Jenkins, Tim G.
Arroyo, Juan A.
author_facet Norton, Carter
Clarke, Derek
Holmstrom, Joshua
Stirland, Isaac
Reynolds, Paul R.
Jenkins, Tim G.
Arroyo, Juan A.
author_sort Norton, Carter
collection PubMed
description Intrauterine growth restriction (IUGR) and preeclampsia (PE) are placental pathologies known to complicate pregnancy and cause neonatal disorders. To date, there is a limited number of studies on the genetic similarity of these conditions. DNA methylation is a heritable epigenetic process that can regulate placental development. Our objective was to identify methylation patterns in placental DNA from normal, PE and IUGR-affected pregnancies. DNA was extracted, and bisulfite was converted, prior to being hybridized for the methylation array. Methylation data were SWAN normalized and differently methylated regions were identified using applications within the USEQ program. UCSC’s Genome browser and Stanford’s GREAT analysis were used to identify gene promoters. The commonality among affected genes was confirmed by Western blot. We observed nine significantly hypomethylated regions, two being significantly hypomethylated for both PE and IGUR. Western blot confirmed differential protein expression of commonly regulated genes. We conclude that despite the uniqueness of methylation profiles for PE and IUGR, the similarity of some methylation alterations in pathologies could explain the clinical similarities observed with these obstetric complications. These results also provide insight into the genetic similarity between PE and IUGR and suggest possible gene candidates plausibly involved in the onset of both conditions.
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spelling pubmed-101364472023-04-28 Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients Norton, Carter Clarke, Derek Holmstrom, Joshua Stirland, Isaac Reynolds, Paul R. Jenkins, Tim G. Arroyo, Juan A. Cells Article Intrauterine growth restriction (IUGR) and preeclampsia (PE) are placental pathologies known to complicate pregnancy and cause neonatal disorders. To date, there is a limited number of studies on the genetic similarity of these conditions. DNA methylation is a heritable epigenetic process that can regulate placental development. Our objective was to identify methylation patterns in placental DNA from normal, PE and IUGR-affected pregnancies. DNA was extracted, and bisulfite was converted, prior to being hybridized for the methylation array. Methylation data were SWAN normalized and differently methylated regions were identified using applications within the USEQ program. UCSC’s Genome browser and Stanford’s GREAT analysis were used to identify gene promoters. The commonality among affected genes was confirmed by Western blot. We observed nine significantly hypomethylated regions, two being significantly hypomethylated for both PE and IGUR. Western blot confirmed differential protein expression of commonly regulated genes. We conclude that despite the uniqueness of methylation profiles for PE and IUGR, the similarity of some methylation alterations in pathologies could explain the clinical similarities observed with these obstetric complications. These results also provide insight into the genetic similarity between PE and IUGR and suggest possible gene candidates plausibly involved in the onset of both conditions. MDPI 2023-04-11 /pmc/articles/PMC10136447/ /pubmed/37190039 http://dx.doi.org/10.3390/cells12081130 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Norton, Carter
Clarke, Derek
Holmstrom, Joshua
Stirland, Isaac
Reynolds, Paul R.
Jenkins, Tim G.
Arroyo, Juan A.
Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients
title Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients
title_full Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients
title_fullStr Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients
title_full_unstemmed Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients
title_short Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients
title_sort altered epigenetic profiles in the placenta of preeclamptic and intrauterine growth restriction patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136447/
https://www.ncbi.nlm.nih.gov/pubmed/37190039
http://dx.doi.org/10.3390/cells12081130
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