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Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)

Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes of death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in the field of diagnostics. In order to enable rapid and accurate measurement of c...

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Autores principales: Tannenberg, Robert, Paul, Martin, Röder, Bettina, Gande, Santosh L., Sreeramulu, Sridhar, Saxena, Krishna, Richter, Christian, Schwalbe, Harald, Swart, Claudia, Weller, Michael G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136549/
https://www.ncbi.nlm.nih.gov/pubmed/37185530
http://dx.doi.org/10.3390/bios13040455
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author Tannenberg, Robert
Paul, Martin
Röder, Bettina
Gande, Santosh L.
Sreeramulu, Sridhar
Saxena, Krishna
Richter, Christian
Schwalbe, Harald
Swart, Claudia
Weller, Michael G.
author_facet Tannenberg, Robert
Paul, Martin
Röder, Bettina
Gande, Santosh L.
Sreeramulu, Sridhar
Saxena, Krishna
Richter, Christian
Schwalbe, Harald
Swart, Claudia
Weller, Michael G.
author_sort Tannenberg, Robert
collection PubMed
description Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes of death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in the field of diagnostics. In order to enable rapid and accurate measurement of cTnI with the potential of online measurements, a chemiluminescence-based immunosensor is presented as a proof of concept. A flow cell was designed and combined with a sensitive CMOS camera allowing sensitive optical readout. In addition, a microfluidic setup was established, which achieved selective and quasi-online cTnI determination within ten minutes. The sensor was tested with recombinant cTnI in phosphate buffer and demonstrated cTnI measurements in the concentration range of 2–25 µg/L. With the optimized system, a limit of detection (LoD) of 0.6 µg/L (23 pmol/L) was achieved. Furthermore, the selectivity of the immunosensor was investigated with other recombinant proteins, such as cTnT, and cTnC, at a level of 16 µg/L. No cross-reactivity could be observed. Measurements with diluted blood plasma and serum resulted in an LoD of 60 µg/L (2.4 nmol/L) and 70 µg/L (2.9 nmol/L), respectively.
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spelling pubmed-101365492023-04-28 Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI) Tannenberg, Robert Paul, Martin Röder, Bettina Gande, Santosh L. Sreeramulu, Sridhar Saxena, Krishna Richter, Christian Schwalbe, Harald Swart, Claudia Weller, Michael G. Biosensors (Basel) Article Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes of death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in the field of diagnostics. In order to enable rapid and accurate measurement of cTnI with the potential of online measurements, a chemiluminescence-based immunosensor is presented as a proof of concept. A flow cell was designed and combined with a sensitive CMOS camera allowing sensitive optical readout. In addition, a microfluidic setup was established, which achieved selective and quasi-online cTnI determination within ten minutes. The sensor was tested with recombinant cTnI in phosphate buffer and demonstrated cTnI measurements in the concentration range of 2–25 µg/L. With the optimized system, a limit of detection (LoD) of 0.6 µg/L (23 pmol/L) was achieved. Furthermore, the selectivity of the immunosensor was investigated with other recombinant proteins, such as cTnT, and cTnC, at a level of 16 µg/L. No cross-reactivity could be observed. Measurements with diluted blood plasma and serum resulted in an LoD of 60 µg/L (2.4 nmol/L) and 70 µg/L (2.9 nmol/L), respectively. MDPI 2023-04-03 /pmc/articles/PMC10136549/ /pubmed/37185530 http://dx.doi.org/10.3390/bios13040455 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tannenberg, Robert
Paul, Martin
Röder, Bettina
Gande, Santosh L.
Sreeramulu, Sridhar
Saxena, Krishna
Richter, Christian
Schwalbe, Harald
Swart, Claudia
Weller, Michael G.
Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)
title Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)
title_full Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)
title_fullStr Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)
title_full_unstemmed Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)
title_short Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)
title_sort chemiluminescence biosensor for the determination of cardiac troponin i (ctni)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136549/
https://www.ncbi.nlm.nih.gov/pubmed/37185530
http://dx.doi.org/10.3390/bios13040455
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