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Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)
Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes of death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in the field of diagnostics. In order to enable rapid and accurate measurement of c...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136549/ https://www.ncbi.nlm.nih.gov/pubmed/37185530 http://dx.doi.org/10.3390/bios13040455 |
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author | Tannenberg, Robert Paul, Martin Röder, Bettina Gande, Santosh L. Sreeramulu, Sridhar Saxena, Krishna Richter, Christian Schwalbe, Harald Swart, Claudia Weller, Michael G. |
author_facet | Tannenberg, Robert Paul, Martin Röder, Bettina Gande, Santosh L. Sreeramulu, Sridhar Saxena, Krishna Richter, Christian Schwalbe, Harald Swart, Claudia Weller, Michael G. |
author_sort | Tannenberg, Robert |
collection | PubMed |
description | Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes of death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in the field of diagnostics. In order to enable rapid and accurate measurement of cTnI with the potential of online measurements, a chemiluminescence-based immunosensor is presented as a proof of concept. A flow cell was designed and combined with a sensitive CMOS camera allowing sensitive optical readout. In addition, a microfluidic setup was established, which achieved selective and quasi-online cTnI determination within ten minutes. The sensor was tested with recombinant cTnI in phosphate buffer and demonstrated cTnI measurements in the concentration range of 2–25 µg/L. With the optimized system, a limit of detection (LoD) of 0.6 µg/L (23 pmol/L) was achieved. Furthermore, the selectivity of the immunosensor was investigated with other recombinant proteins, such as cTnT, and cTnC, at a level of 16 µg/L. No cross-reactivity could be observed. Measurements with diluted blood plasma and serum resulted in an LoD of 60 µg/L (2.4 nmol/L) and 70 µg/L (2.9 nmol/L), respectively. |
format | Online Article Text |
id | pubmed-10136549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101365492023-04-28 Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI) Tannenberg, Robert Paul, Martin Röder, Bettina Gande, Santosh L. Sreeramulu, Sridhar Saxena, Krishna Richter, Christian Schwalbe, Harald Swart, Claudia Weller, Michael G. Biosensors (Basel) Article Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes of death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in the field of diagnostics. In order to enable rapid and accurate measurement of cTnI with the potential of online measurements, a chemiluminescence-based immunosensor is presented as a proof of concept. A flow cell was designed and combined with a sensitive CMOS camera allowing sensitive optical readout. In addition, a microfluidic setup was established, which achieved selective and quasi-online cTnI determination within ten minutes. The sensor was tested with recombinant cTnI in phosphate buffer and demonstrated cTnI measurements in the concentration range of 2–25 µg/L. With the optimized system, a limit of detection (LoD) of 0.6 µg/L (23 pmol/L) was achieved. Furthermore, the selectivity of the immunosensor was investigated with other recombinant proteins, such as cTnT, and cTnC, at a level of 16 µg/L. No cross-reactivity could be observed. Measurements with diluted blood plasma and serum resulted in an LoD of 60 µg/L (2.4 nmol/L) and 70 µg/L (2.9 nmol/L), respectively. MDPI 2023-04-03 /pmc/articles/PMC10136549/ /pubmed/37185530 http://dx.doi.org/10.3390/bios13040455 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tannenberg, Robert Paul, Martin Röder, Bettina Gande, Santosh L. Sreeramulu, Sridhar Saxena, Krishna Richter, Christian Schwalbe, Harald Swart, Claudia Weller, Michael G. Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI) |
title | Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI) |
title_full | Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI) |
title_fullStr | Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI) |
title_full_unstemmed | Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI) |
title_short | Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI) |
title_sort | chemiluminescence biosensor for the determination of cardiac troponin i (ctni) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136549/ https://www.ncbi.nlm.nih.gov/pubmed/37185530 http://dx.doi.org/10.3390/bios13040455 |
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