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How Does the Sample Preparation of Phytophthora infestans Mycelium Affect the Quality of Isolated RNA?
RNA isolation from fungi and fungus-like organisms is not an easy task. Active endogenous RNases quickly hydrolyze RNA after the sample collection, and the thick cell wall prevents inhibitors from penetrating the cells. Therefore, the initial collection and grinding steps may be crucial for the tota...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MDPI
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136579/ https://www.ncbi.nlm.nih.gov/pubmed/37185754 http://dx.doi.org/10.3390/cimb45040230 |