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Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion

Rosacea is a chronic inflammatory skin disease whose late manifestations have not yet been clearly reported in animal models. The objective of this study is to describe the skin lesions and major histopathological changes in a rosacea-like phenotype in mice induced by prolonged LL-37 administration...

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Autores principales: Zhang, Chuanxi, Kang, Yumeng, Zhang, Ziyan, Liu, Heliang, Xu, Hong, Cai, Wenchen, Gao, Xuemin, Yang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136735/
https://www.ncbi.nlm.nih.gov/pubmed/37185701
http://dx.doi.org/10.3390/cimb45040177
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author Zhang, Chuanxi
Kang, Yumeng
Zhang, Ziyan
Liu, Heliang
Xu, Hong
Cai, Wenchen
Gao, Xuemin
Yang, Jie
author_facet Zhang, Chuanxi
Kang, Yumeng
Zhang, Ziyan
Liu, Heliang
Xu, Hong
Cai, Wenchen
Gao, Xuemin
Yang, Jie
author_sort Zhang, Chuanxi
collection PubMed
description Rosacea is a chronic inflammatory skin disease whose late manifestations have not yet been clearly reported in animal models. The objective of this study is to describe the skin lesions and major histopathological changes in a rosacea-like phenotype in mice induced by prolonged LL-37 administration and furthermore, to assess the potential of long-term LL-37 administration in inducing irreversible rosacea-like skin lesion models. Balb/c mice were continuously injected intradermally with LL-37 every 12 h to induce a rosacea-like phenotype. After LL-37 injections were administered for 20 consecutive days, the area of rosacea-like lesions gradually expanded in the first 13 days, then entered a stable phase. Haematoxylin and eosin (H&E) and Van Gieson’s staining showed a high degree of inflammatory cell aggregation, thickening of the epidermis and dermis, and collagen deposition in large quantities. The results of immunofluorescence staining and Western blotting showed that the expression of α-SMA, TNF-α, vimentin, and COL1 in the skin of mice was significantly upregulated. Short-term LL-37 administration induced rosacea-like lesions that only featured the aggregation of inflammatory factors and thickening of the epidermis, whereas no collagen hyperplasia was observed, and a full recovery was noticed. However, rosacea-like skin lesions induced by long-term LL-37 administration did not completely recover. Our study compares rosacea-like lesions induced by short-term versus long-term LL-37 administration, and the results suggest that irreversible rosacea-like lesions can be induced by long-term LL-37 administration.
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spelling pubmed-101367352023-04-28 Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion Zhang, Chuanxi Kang, Yumeng Zhang, Ziyan Liu, Heliang Xu, Hong Cai, Wenchen Gao, Xuemin Yang, Jie Curr Issues Mol Biol Article Rosacea is a chronic inflammatory skin disease whose late manifestations have not yet been clearly reported in animal models. The objective of this study is to describe the skin lesions and major histopathological changes in a rosacea-like phenotype in mice induced by prolonged LL-37 administration and furthermore, to assess the potential of long-term LL-37 administration in inducing irreversible rosacea-like skin lesion models. Balb/c mice were continuously injected intradermally with LL-37 every 12 h to induce a rosacea-like phenotype. After LL-37 injections were administered for 20 consecutive days, the area of rosacea-like lesions gradually expanded in the first 13 days, then entered a stable phase. Haematoxylin and eosin (H&E) and Van Gieson’s staining showed a high degree of inflammatory cell aggregation, thickening of the epidermis and dermis, and collagen deposition in large quantities. The results of immunofluorescence staining and Western blotting showed that the expression of α-SMA, TNF-α, vimentin, and COL1 in the skin of mice was significantly upregulated. Short-term LL-37 administration induced rosacea-like lesions that only featured the aggregation of inflammatory factors and thickening of the epidermis, whereas no collagen hyperplasia was observed, and a full recovery was noticed. However, rosacea-like skin lesions induced by long-term LL-37 administration did not completely recover. Our study compares rosacea-like lesions induced by short-term versus long-term LL-37 administration, and the results suggest that irreversible rosacea-like lesions can be induced by long-term LL-37 administration. MDPI 2023-03-24 /pmc/articles/PMC10136735/ /pubmed/37185701 http://dx.doi.org/10.3390/cimb45040177 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Chuanxi
Kang, Yumeng
Zhang, Ziyan
Liu, Heliang
Xu, Hong
Cai, Wenchen
Gao, Xuemin
Yang, Jie
Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion
title Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion
title_full Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion
title_fullStr Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion
title_full_unstemmed Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion
title_short Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion
title_sort long-term administration of ll-37 can induce irreversible rosacea-like lesion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136735/
https://www.ncbi.nlm.nih.gov/pubmed/37185701
http://dx.doi.org/10.3390/cimb45040177
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