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A mouse ear skin model to study the dynamics of innate immune responses against the microsporidian Encephalitozoon cuniculi

Microsporidia are obligate intracellular parasites related to fungi that cause severe infections in immunocompromised individuals. Encephalitozoon cuniculi is a microsporidian species capable of infecting mammals, including human and rodents. In response to microsporidian infection, innate immune sy...

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Autores principales: Carriere, Eugénie, Abdul Hamid, Aizat Iman, Feki, Inès, Dubuffet, Aurore, Delbac, Frédéric, Gueirard, Pascale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136781/
https://www.ncbi.nlm.nih.gov/pubmed/37125152
http://dx.doi.org/10.3389/fmicb.2023.1168970
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author Carriere, Eugénie
Abdul Hamid, Aizat Iman
Feki, Inès
Dubuffet, Aurore
Delbac, Frédéric
Gueirard, Pascale
author_facet Carriere, Eugénie
Abdul Hamid, Aizat Iman
Feki, Inès
Dubuffet, Aurore
Delbac, Frédéric
Gueirard, Pascale
author_sort Carriere, Eugénie
collection PubMed
description Microsporidia are obligate intracellular parasites related to fungi that cause severe infections in immunocompromised individuals. Encephalitozoon cuniculi is a microsporidian species capable of infecting mammals, including human and rodents. In response to microsporidian infection, innate immune system serves as the first line of defense and allows a partial clearance of the parasite via the innate immune cells, namely macrophages, neutrophils, dendritic cells, and Natural Killer cells. According to the literature, microsporidia bypass this response in vitro by modulating the response of macrophages. In order to study host-parasites interactions in vivo, we developed a model using the mouse ear pinna in combination with an intravital imaging approach. Fluorescent E. cuniculi spores were inoculated into the skin tissue to follow for the first time in real time in an in vivo model the recruitment dynamics of EGFP + phagocytic cells in response to the parasite. The results show that parasites induce an important inflammatory recruitment of phagocytes, with alterations of their motility properties (speed, displacement length, straightness). This cellular response persists in the injection zone, with spores detected inside the phagocytes up to 72 h post-infection. Immunostainings performed on ear tissue cryosections evoke the presence of developing infectious foci from 5 days post-infection, in favor of parasite proliferation in this tissue. Overall, the newly set up mice ear pinna model will increase our understanding of the immunobiology of microsporidia and in particular, to know how they can bypass and hijack the host immune system of an immunocompetent or immunosuppressed host.
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spelling pubmed-101367812023-04-28 A mouse ear skin model to study the dynamics of innate immune responses against the microsporidian Encephalitozoon cuniculi Carriere, Eugénie Abdul Hamid, Aizat Iman Feki, Inès Dubuffet, Aurore Delbac, Frédéric Gueirard, Pascale Front Microbiol Microbiology Microsporidia are obligate intracellular parasites related to fungi that cause severe infections in immunocompromised individuals. Encephalitozoon cuniculi is a microsporidian species capable of infecting mammals, including human and rodents. In response to microsporidian infection, innate immune system serves as the first line of defense and allows a partial clearance of the parasite via the innate immune cells, namely macrophages, neutrophils, dendritic cells, and Natural Killer cells. According to the literature, microsporidia bypass this response in vitro by modulating the response of macrophages. In order to study host-parasites interactions in vivo, we developed a model using the mouse ear pinna in combination with an intravital imaging approach. Fluorescent E. cuniculi spores were inoculated into the skin tissue to follow for the first time in real time in an in vivo model the recruitment dynamics of EGFP + phagocytic cells in response to the parasite. The results show that parasites induce an important inflammatory recruitment of phagocytes, with alterations of their motility properties (speed, displacement length, straightness). This cellular response persists in the injection zone, with spores detected inside the phagocytes up to 72 h post-infection. Immunostainings performed on ear tissue cryosections evoke the presence of developing infectious foci from 5 days post-infection, in favor of parasite proliferation in this tissue. Overall, the newly set up mice ear pinna model will increase our understanding of the immunobiology of microsporidia and in particular, to know how they can bypass and hijack the host immune system of an immunocompetent or immunosuppressed host. Frontiers Media S.A. 2023-04-13 /pmc/articles/PMC10136781/ /pubmed/37125152 http://dx.doi.org/10.3389/fmicb.2023.1168970 Text en Copyright © 2023 Carriere, Abdul Hamid, Feki, Dubuffet, Delbac and Gueirard. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Carriere, Eugénie
Abdul Hamid, Aizat Iman
Feki, Inès
Dubuffet, Aurore
Delbac, Frédéric
Gueirard, Pascale
A mouse ear skin model to study the dynamics of innate immune responses against the microsporidian Encephalitozoon cuniculi
title A mouse ear skin model to study the dynamics of innate immune responses against the microsporidian Encephalitozoon cuniculi
title_full A mouse ear skin model to study the dynamics of innate immune responses against the microsporidian Encephalitozoon cuniculi
title_fullStr A mouse ear skin model to study the dynamics of innate immune responses against the microsporidian Encephalitozoon cuniculi
title_full_unstemmed A mouse ear skin model to study the dynamics of innate immune responses against the microsporidian Encephalitozoon cuniculi
title_short A mouse ear skin model to study the dynamics of innate immune responses against the microsporidian Encephalitozoon cuniculi
title_sort mouse ear skin model to study the dynamics of innate immune responses against the microsporidian encephalitozoon cuniculi
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136781/
https://www.ncbi.nlm.nih.gov/pubmed/37125152
http://dx.doi.org/10.3389/fmicb.2023.1168970
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