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Inhibitory Effect of Phosphorothioate Oligonucleotide Complementary to G6PD mRNA on Murine Melanoma
In terms of the incidence among all tumors, skin cancer is on top, with the most deadly among them being melanoma. The search for new therapeutic agents to combat melanoma is very relevant. In our opinion, antisense oligonucleotides (ASO) aimed at suppressing the genes responsible for their viabilit...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137061/ https://www.ncbi.nlm.nih.gov/pubmed/37185731 http://dx.doi.org/10.3390/cimb45040207 |
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author | Yurchenko, Kseniya A. Laikova, Kateryna V. Golovkin, Ilya O. Novikov, Ilya A. Yurchenko, Alyona A. Makalish, Tatyana P. Oberemok, Volodymyr V. |
author_facet | Yurchenko, Kseniya A. Laikova, Kateryna V. Golovkin, Ilya O. Novikov, Ilya A. Yurchenko, Alyona A. Makalish, Tatyana P. Oberemok, Volodymyr V. |
author_sort | Yurchenko, Kseniya A. |
collection | PubMed |
description | In terms of the incidence among all tumors, skin cancer is on top, with the most deadly among them being melanoma. The search for new therapeutic agents to combat melanoma is very relevant. In our opinion, antisense oligonucleotides (ASO) aimed at suppressing the genes responsible for their viability in cancer cells give hope for treatment, which makes it possible to eliminate cancer cells near the tumor site both before and after surgery. In this article, we describe how Skeen-11 phosphorothioate oligonucleotide significantly decreased the proliferative activity of murine melanoma cells. Injections of Skeen-11 also inhibited tumor growth in mice with inoculated melanoma. A toxicity study showed no side effects with dose adjustments. The results show that the use of ASO Skeen-11 in vivo reduced the tumor size within 7 days, reduced the number of mitoses in the tumor cells, and increased the amount of necrosis compared with the control group. |
format | Online Article Text |
id | pubmed-10137061 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101370612023-04-28 Inhibitory Effect of Phosphorothioate Oligonucleotide Complementary to G6PD mRNA on Murine Melanoma Yurchenko, Kseniya A. Laikova, Kateryna V. Golovkin, Ilya O. Novikov, Ilya A. Yurchenko, Alyona A. Makalish, Tatyana P. Oberemok, Volodymyr V. Curr Issues Mol Biol Article In terms of the incidence among all tumors, skin cancer is on top, with the most deadly among them being melanoma. The search for new therapeutic agents to combat melanoma is very relevant. In our opinion, antisense oligonucleotides (ASO) aimed at suppressing the genes responsible for their viability in cancer cells give hope for treatment, which makes it possible to eliminate cancer cells near the tumor site both before and after surgery. In this article, we describe how Skeen-11 phosphorothioate oligonucleotide significantly decreased the proliferative activity of murine melanoma cells. Injections of Skeen-11 also inhibited tumor growth in mice with inoculated melanoma. A toxicity study showed no side effects with dose adjustments. The results show that the use of ASO Skeen-11 in vivo reduced the tumor size within 7 days, reduced the number of mitoses in the tumor cells, and increased the amount of necrosis compared with the control group. MDPI 2023-04-06 /pmc/articles/PMC10137061/ /pubmed/37185731 http://dx.doi.org/10.3390/cimb45040207 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yurchenko, Kseniya A. Laikova, Kateryna V. Golovkin, Ilya O. Novikov, Ilya A. Yurchenko, Alyona A. Makalish, Tatyana P. Oberemok, Volodymyr V. Inhibitory Effect of Phosphorothioate Oligonucleotide Complementary to G6PD mRNA on Murine Melanoma |
title | Inhibitory Effect of Phosphorothioate Oligonucleotide Complementary to G6PD mRNA on Murine Melanoma |
title_full | Inhibitory Effect of Phosphorothioate Oligonucleotide Complementary to G6PD mRNA on Murine Melanoma |
title_fullStr | Inhibitory Effect of Phosphorothioate Oligonucleotide Complementary to G6PD mRNA on Murine Melanoma |
title_full_unstemmed | Inhibitory Effect of Phosphorothioate Oligonucleotide Complementary to G6PD mRNA on Murine Melanoma |
title_short | Inhibitory Effect of Phosphorothioate Oligonucleotide Complementary to G6PD mRNA on Murine Melanoma |
title_sort | inhibitory effect of phosphorothioate oligonucleotide complementary to g6pd mrna on murine melanoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137061/ https://www.ncbi.nlm.nih.gov/pubmed/37185731 http://dx.doi.org/10.3390/cimb45040207 |
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