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Longitudinal Study for the Detection and Quantification of Campylobacter spp. in Dairy Cows during Milking and in the Dairy Farm Environment

Campylobacteriosis outbreaks have repeatedly been associated with the consumption of raw milk. This study aimed to explore the variation in the prevalence and concentration of Campylobacter spp. in cows’ milk and feces, the farm environment and on the teat skin over an entire year at a small German...

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Autores principales: Knipper, Anna-Delia, Göhlich, Steven, Stingl, Kerstin, Ghoreishi, Narges, Fischer-Tenhagen, Carola, Bandick, Niels, Tenhagen, Bernd-Alois, Crease, Tasja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137412/
https://www.ncbi.nlm.nih.gov/pubmed/37107434
http://dx.doi.org/10.3390/foods12081639
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author Knipper, Anna-Delia
Göhlich, Steven
Stingl, Kerstin
Ghoreishi, Narges
Fischer-Tenhagen, Carola
Bandick, Niels
Tenhagen, Bernd-Alois
Crease, Tasja
author_facet Knipper, Anna-Delia
Göhlich, Steven
Stingl, Kerstin
Ghoreishi, Narges
Fischer-Tenhagen, Carola
Bandick, Niels
Tenhagen, Bernd-Alois
Crease, Tasja
author_sort Knipper, Anna-Delia
collection PubMed
description Campylobacteriosis outbreaks have repeatedly been associated with the consumption of raw milk. This study aimed to explore the variation in the prevalence and concentration of Campylobacter spp. in cows’ milk and feces, the farm environment and on the teat skin over an entire year at a small German dairy farm. Bi-weekly samples were collected from the environment (boot socks), teats, raw milk, milk filters, milking clusters and feces collected from the recta of dairy cows. Samples were analyzed for Campylobacter spp., E. coli, the total aerobic plate count and for Pseudomonas spp. The prevalence of Campylobacter spp. was found to be the highest in feces (77.1%), completely absent in milking equipment and low in raw milk (0.4%). The mean concentration of Campylobacter spp. was 2.43 log(10) colony-forming units (CFU)/g in feces and 1.26 log(10) CFU/teat swab. Only a single milk filter at the end of the milk pipeline and one individual cow’s raw milk sample were positive on the same day, with a concentration of 2.74 log(10) CFU/filter and 2.37 log(10) CFU/mL for the raw milk. On the same day, nine teat swab samples tested positive for Campylobacter spp. This study highlights the persistence of Campylobacter spp. for at least one year in the intestine of individual cows and within the general farm environment and demonstrates that fecal cross-contamination of the teats can occur even when the contamination of raw milk is a rare event.
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spelling pubmed-101374122023-04-28 Longitudinal Study for the Detection and Quantification of Campylobacter spp. in Dairy Cows during Milking and in the Dairy Farm Environment Knipper, Anna-Delia Göhlich, Steven Stingl, Kerstin Ghoreishi, Narges Fischer-Tenhagen, Carola Bandick, Niels Tenhagen, Bernd-Alois Crease, Tasja Foods Article Campylobacteriosis outbreaks have repeatedly been associated with the consumption of raw milk. This study aimed to explore the variation in the prevalence and concentration of Campylobacter spp. in cows’ milk and feces, the farm environment and on the teat skin over an entire year at a small German dairy farm. Bi-weekly samples were collected from the environment (boot socks), teats, raw milk, milk filters, milking clusters and feces collected from the recta of dairy cows. Samples were analyzed for Campylobacter spp., E. coli, the total aerobic plate count and for Pseudomonas spp. The prevalence of Campylobacter spp. was found to be the highest in feces (77.1%), completely absent in milking equipment and low in raw milk (0.4%). The mean concentration of Campylobacter spp. was 2.43 log(10) colony-forming units (CFU)/g in feces and 1.26 log(10) CFU/teat swab. Only a single milk filter at the end of the milk pipeline and one individual cow’s raw milk sample were positive on the same day, with a concentration of 2.74 log(10) CFU/filter and 2.37 log(10) CFU/mL for the raw milk. On the same day, nine teat swab samples tested positive for Campylobacter spp. This study highlights the persistence of Campylobacter spp. for at least one year in the intestine of individual cows and within the general farm environment and demonstrates that fecal cross-contamination of the teats can occur even when the contamination of raw milk is a rare event. MDPI 2023-04-13 /pmc/articles/PMC10137412/ /pubmed/37107434 http://dx.doi.org/10.3390/foods12081639 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Knipper, Anna-Delia
Göhlich, Steven
Stingl, Kerstin
Ghoreishi, Narges
Fischer-Tenhagen, Carola
Bandick, Niels
Tenhagen, Bernd-Alois
Crease, Tasja
Longitudinal Study for the Detection and Quantification of Campylobacter spp. in Dairy Cows during Milking and in the Dairy Farm Environment
title Longitudinal Study for the Detection and Quantification of Campylobacter spp. in Dairy Cows during Milking and in the Dairy Farm Environment
title_full Longitudinal Study for the Detection and Quantification of Campylobacter spp. in Dairy Cows during Milking and in the Dairy Farm Environment
title_fullStr Longitudinal Study for the Detection and Quantification of Campylobacter spp. in Dairy Cows during Milking and in the Dairy Farm Environment
title_full_unstemmed Longitudinal Study for the Detection and Quantification of Campylobacter spp. in Dairy Cows during Milking and in the Dairy Farm Environment
title_short Longitudinal Study for the Detection and Quantification of Campylobacter spp. in Dairy Cows during Milking and in the Dairy Farm Environment
title_sort longitudinal study for the detection and quantification of campylobacter spp. in dairy cows during milking and in the dairy farm environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137412/
https://www.ncbi.nlm.nih.gov/pubmed/37107434
http://dx.doi.org/10.3390/foods12081639
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