Cargando…

Osteogenesis Enhancement with 3D Printed Gene-Activated Sodium Alginate Scaffolds

Natural and synthetic hydrogel scaffolds containing bioactive components are increasingly used in solving various tissue engineering problems. The encapsulation of DNA-encoding osteogenic growth factors with transfecting agents (e.g., polyplexes) into such scaffold structures is one of the promising...

Descripción completa

Detalles Bibliográficos
Autores principales: Khvorostina, Maria, Mironov, Anton, Nedorubova, Irina, Bukharova, Tatiana, Vasilyev, Andrey, Goldshtein, Dmitry, Komlev, Vladimir, Popov, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137500/
https://www.ncbi.nlm.nih.gov/pubmed/37102926
http://dx.doi.org/10.3390/gels9040315
_version_ 1785032479426478080
author Khvorostina, Maria
Mironov, Anton
Nedorubova, Irina
Bukharova, Tatiana
Vasilyev, Andrey
Goldshtein, Dmitry
Komlev, Vladimir
Popov, Vladimir
author_facet Khvorostina, Maria
Mironov, Anton
Nedorubova, Irina
Bukharova, Tatiana
Vasilyev, Andrey
Goldshtein, Dmitry
Komlev, Vladimir
Popov, Vladimir
author_sort Khvorostina, Maria
collection PubMed
description Natural and synthetic hydrogel scaffolds containing bioactive components are increasingly used in solving various tissue engineering problems. The encapsulation of DNA-encoding osteogenic growth factors with transfecting agents (e.g., polyplexes) into such scaffold structures is one of the promising approaches to delivering the corresponding genes to the area of the bone defect to be replaced, providing the prolonged expression of the required proteins. Herein, a comparative assessment of both in vitro and in vivo osteogenic properties of 3D printed sodium alginate (SA) hydrogel scaffolds impregnated with model EGFP and therapeutic BMP-2 plasmids was demonstrated for the first time. The expression levels of mesenchymal stem cell (MSC) osteogenic differentiation markers Runx2, Alpl, and Bglap were evaluated by real-time PCR. Osteogenesis in vivo was studied on a model of a critical-sized cranial defect in Wistar rats using micro-CT and histomorphology. The incorporation of polyplexes comprising pEGFP and pBMP-2 plasmids into the SA solution followed by 3D cryoprinting does not affect their transfecting ability compared to the initial compounds. Histomorphometry and micro-CT analysis 8 weeks after scaffold implantation manifested a significant (up to 46%) increase in new bone volume formation for the SA/pBMP-2 scaffolds compared to the SA/pEGFP ones.
format Online
Article
Text
id pubmed-10137500
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101375002023-04-28 Osteogenesis Enhancement with 3D Printed Gene-Activated Sodium Alginate Scaffolds Khvorostina, Maria Mironov, Anton Nedorubova, Irina Bukharova, Tatiana Vasilyev, Andrey Goldshtein, Dmitry Komlev, Vladimir Popov, Vladimir Gels Article Natural and synthetic hydrogel scaffolds containing bioactive components are increasingly used in solving various tissue engineering problems. The encapsulation of DNA-encoding osteogenic growth factors with transfecting agents (e.g., polyplexes) into such scaffold structures is one of the promising approaches to delivering the corresponding genes to the area of the bone defect to be replaced, providing the prolonged expression of the required proteins. Herein, a comparative assessment of both in vitro and in vivo osteogenic properties of 3D printed sodium alginate (SA) hydrogel scaffolds impregnated with model EGFP and therapeutic BMP-2 plasmids was demonstrated for the first time. The expression levels of mesenchymal stem cell (MSC) osteogenic differentiation markers Runx2, Alpl, and Bglap were evaluated by real-time PCR. Osteogenesis in vivo was studied on a model of a critical-sized cranial defect in Wistar rats using micro-CT and histomorphology. The incorporation of polyplexes comprising pEGFP and pBMP-2 plasmids into the SA solution followed by 3D cryoprinting does not affect their transfecting ability compared to the initial compounds. Histomorphometry and micro-CT analysis 8 weeks after scaffold implantation manifested a significant (up to 46%) increase in new bone volume formation for the SA/pBMP-2 scaffolds compared to the SA/pEGFP ones. MDPI 2023-04-07 /pmc/articles/PMC10137500/ /pubmed/37102926 http://dx.doi.org/10.3390/gels9040315 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khvorostina, Maria
Mironov, Anton
Nedorubova, Irina
Bukharova, Tatiana
Vasilyev, Andrey
Goldshtein, Dmitry
Komlev, Vladimir
Popov, Vladimir
Osteogenesis Enhancement with 3D Printed Gene-Activated Sodium Alginate Scaffolds
title Osteogenesis Enhancement with 3D Printed Gene-Activated Sodium Alginate Scaffolds
title_full Osteogenesis Enhancement with 3D Printed Gene-Activated Sodium Alginate Scaffolds
title_fullStr Osteogenesis Enhancement with 3D Printed Gene-Activated Sodium Alginate Scaffolds
title_full_unstemmed Osteogenesis Enhancement with 3D Printed Gene-Activated Sodium Alginate Scaffolds
title_short Osteogenesis Enhancement with 3D Printed Gene-Activated Sodium Alginate Scaffolds
title_sort osteogenesis enhancement with 3d printed gene-activated sodium alginate scaffolds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137500/
https://www.ncbi.nlm.nih.gov/pubmed/37102926
http://dx.doi.org/10.3390/gels9040315
work_keys_str_mv AT khvorostinamaria osteogenesisenhancementwith3dprintedgeneactivatedsodiumalginatescaffolds
AT mironovanton osteogenesisenhancementwith3dprintedgeneactivatedsodiumalginatescaffolds
AT nedorubovairina osteogenesisenhancementwith3dprintedgeneactivatedsodiumalginatescaffolds
AT bukharovatatiana osteogenesisenhancementwith3dprintedgeneactivatedsodiumalginatescaffolds
AT vasilyevandrey osteogenesisenhancementwith3dprintedgeneactivatedsodiumalginatescaffolds
AT goldshteindmitry osteogenesisenhancementwith3dprintedgeneactivatedsodiumalginatescaffolds
AT komlevvladimir osteogenesisenhancementwith3dprintedgeneactivatedsodiumalginatescaffolds
AT popovvladimir osteogenesisenhancementwith3dprintedgeneactivatedsodiumalginatescaffolds