tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue
Noncoding RNAs (ncRNAs) called tsRNAs (tRNA-derived short RNAs) have the ability to regulate gene expression. The information on tsRNAs in fat tissue is, however, limited. By sequencing, identifying, and analyzing tsRNAs using pigs as animal models, this research reports for the first time the chara...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137714/ https://www.ncbi.nlm.nih.gov/pubmed/37107540 http://dx.doi.org/10.3390/genes14040782 |
_version_ | 1785032533352644608 |
---|---|
author | Wang, Linghui Gu, Hao Liao, Tianci Lei, Yuhang Qiu, Yanhao Chen, Qiuyang Chen, Lei Zhang, Shunhua Wang, Jinyong Hao, Xiaoxia Jiang, Dongmei Zhao, Ye Niu, Lili Li, Xuewei Shen, Linyuan Gan, Mailin Zhu, Li |
author_facet | Wang, Linghui Gu, Hao Liao, Tianci Lei, Yuhang Qiu, Yanhao Chen, Qiuyang Chen, Lei Zhang, Shunhua Wang, Jinyong Hao, Xiaoxia Jiang, Dongmei Zhao, Ye Niu, Lili Li, Xuewei Shen, Linyuan Gan, Mailin Zhu, Li |
author_sort | Wang, Linghui |
collection | PubMed |
description | Noncoding RNAs (ncRNAs) called tsRNAs (tRNA-derived short RNAs) have the ability to regulate gene expression. The information on tsRNAs in fat tissue is, however, limited. By sequencing, identifying, and analyzing tsRNAs using pigs as animal models, this research reports for the first time the characteristics of tsRNAs in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT). A total of 474 tsRNAs, 20 and 21 of which were particularly expressed in VAT and SAT, respectively, were found in WAT. According to the analysis of the tsRNA/miRNA/mRNA co-expression network, the tsRNAs with differential expression were primarily engaged in the endocrine and immune systems, which fall under the classification of organic systems, as well as the global and overview maps and lipid metropolis, which fall under the category of metabolism. This research also discovered a connection between the activity of the host tRNA engaged in translation and the production of tsRNAs. This research also discovered that tRF-Gly-GCC-037/tRF-Gly-GCC-042/tRF-Gly-CCC-016 and miR-218a/miR281b may be involved in the regulation of fatty acid metabolism in adipose tissue through SCD based on the tsRNA/miRNA/mRNA/fatty acid network. In conclusion, our findings enrich the understanding of ncRNAs in WAT metabolism and health regulation, as well as reveal the differences between SAT and VAT at the level of tsRNAs. |
format | Online Article Text |
id | pubmed-10137714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101377142023-04-28 tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue Wang, Linghui Gu, Hao Liao, Tianci Lei, Yuhang Qiu, Yanhao Chen, Qiuyang Chen, Lei Zhang, Shunhua Wang, Jinyong Hao, Xiaoxia Jiang, Dongmei Zhao, Ye Niu, Lili Li, Xuewei Shen, Linyuan Gan, Mailin Zhu, Li Genes (Basel) Article Noncoding RNAs (ncRNAs) called tsRNAs (tRNA-derived short RNAs) have the ability to regulate gene expression. The information on tsRNAs in fat tissue is, however, limited. By sequencing, identifying, and analyzing tsRNAs using pigs as animal models, this research reports for the first time the characteristics of tsRNAs in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT). A total of 474 tsRNAs, 20 and 21 of which were particularly expressed in VAT and SAT, respectively, were found in WAT. According to the analysis of the tsRNA/miRNA/mRNA co-expression network, the tsRNAs with differential expression were primarily engaged in the endocrine and immune systems, which fall under the classification of organic systems, as well as the global and overview maps and lipid metropolis, which fall under the category of metabolism. This research also discovered a connection between the activity of the host tRNA engaged in translation and the production of tsRNAs. This research also discovered that tRF-Gly-GCC-037/tRF-Gly-GCC-042/tRF-Gly-CCC-016 and miR-218a/miR281b may be involved in the regulation of fatty acid metabolism in adipose tissue through SCD based on the tsRNA/miRNA/mRNA/fatty acid network. In conclusion, our findings enrich the understanding of ncRNAs in WAT metabolism and health regulation, as well as reveal the differences between SAT and VAT at the level of tsRNAs. MDPI 2023-03-23 /pmc/articles/PMC10137714/ /pubmed/37107540 http://dx.doi.org/10.3390/genes14040782 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Linghui Gu, Hao Liao, Tianci Lei, Yuhang Qiu, Yanhao Chen, Qiuyang Chen, Lei Zhang, Shunhua Wang, Jinyong Hao, Xiaoxia Jiang, Dongmei Zhao, Ye Niu, Lili Li, Xuewei Shen, Linyuan Gan, Mailin Zhu, Li tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue |
title | tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue |
title_full | tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue |
title_fullStr | tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue |
title_full_unstemmed | tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue |
title_short | tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue |
title_sort | tsrna landscape and potential function network in subcutaneous and visceral pig adipose tissue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137714/ https://www.ncbi.nlm.nih.gov/pubmed/37107540 http://dx.doi.org/10.3390/genes14040782 |
work_keys_str_mv | AT wanglinghui tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT guhao tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT liaotianci tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT leiyuhang tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT qiuyanhao tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT chenqiuyang tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT chenlei tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT zhangshunhua tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT wangjinyong tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT haoxiaoxia tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT jiangdongmei tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT zhaoye tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT niulili tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT lixuewei tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT shenlinyuan tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT ganmailin tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue AT zhuli tsrnalandscapeandpotentialfunctionnetworkinsubcutaneousandvisceralpigadiposetissue |