Cargando…

Targeted Modulation of Chicken Genes In Vitro Using CRISPRa and CRISPRi Toolkit

Engineering of clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated protein 9 (Cas9) system has enabled versatile applications of CRISPR beyond targeted DNA cleavage. Combination of nuclease-deactivated Cas9 (dCas9) and transcriptional effector domains allows...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chapman, Brittany, Han, Jeong Hoon, Lee, Hong Jo, Ruud, Isabella, Kim, Tae Hyun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137795/ https://www.ncbi.nlm.nih.gov/pubmed/37107664 http://dx.doi.org/10.3390/genes14040906

Ejemplares similares

Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi
por: Jensen, Trine I., et al.
Publicado: (2021)

Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation
por: Chen, Li, et al.
Publicado: (2020)

A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells
por: Hazelbaker, Dane Z., et al.
Publicado: (2020)

Using CRISPRa and CRISPRi Technologies to Study the Biological Functions of ITGB5, TIMP1, and TMEM176B in Prostate Cancer Cells
por: Yang, Yi, et al.
Publicado: (2021)

Promoting CRISPRa for Targeted Treatment of Epilepsy
por: Wagnon, Jacy L.
Publicado: (2020)

Off-target effects of CRISPRa on interleukin-6 expression
por: Soubeyrand, Sébastien, et al.
Publicado: (2019)

A Modular Cloning Toolkit Including CRISPRi for the Engineering of the Human Fungal Pathogen and Biotechnology Host Candida glabrata
por: Billerbeck, Sonja, et al.
Publicado: (2023)

Tumour suppression by targeted intravenous non-viral CRISPRa using dendritic polymers
por: Kretzmann, Jessica A., et al.
Publicado: (2019)

Porcine antiviral activity is increased by CRISPRa-SAM system
por: Jiang, Jinhe, et al.
Publicado: (2019)

Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus
por: Kostyushev, Dmitry, et al.
Publicado: (2023)

CRISPRa-mediated FOXP3 gene upregulation in mammalian cells
por: Forstnerič, Vida, et al.
Publicado: (2019)

Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa
por: Lau, Cia-Hin, et al.
Publicado: (2019)

Transcriptional modulation of AREB-1 by CRISPRa improves plant physiological performance under severe water deficit
por: de Melo, Bruno Paes, et al.
Publicado: (2020)

CRISPRi screens reveal genes modulating yeast growth in lignocellulose hydrolysate
por: Gutmann, Friederike, et al.
Publicado: (2021)

Characterization of transcriptional enhancers in the chicken genome using CRISPR-mediated activation
por: Han, Jeong Hoon, et al.
Publicado: (2023)

Bidirectional manipulation of gene expression in adipocytes using CRISPRa and siRNA
por: Lundh, Morten, et al.
Publicado: (2017)

Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria
por: Liu, Yang, et al.
Publicado: (2019)

Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming
por: Yang, Jian, et al.
Publicado: (2019)

Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits
por: Alba Burbano, Diego, et al.
Publicado: (2023)

Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
por: Fontana, Jason, et al.
Publicado: (2020)

DIPG-03. IDENTIFICATION OF NEW THERAPEUTIC TARGETS BY ARRAYED CRISPRA-BASED APPROACH TO MAP RADIORESISTANCE IN DMG
por: Fernandez, Solène, et al.
Publicado: (2023)

An improved CRISPRi system in Pichia pastoris
por: Qiao, Shujing, et al.
Publicado: (2023)

Systematic functional interrogation of human pseudogenes using CRISPRi
por: Sun, Ming, et al.
Publicado: (2021)

CRISPRi Gene Modulation and All-Optical Electrophysiology in Post-Differentiated Human iPSC-Cardiomyocytes
por: Han, Julie L., et al.
Publicado: (2023)

Multiplexed activation of endogenous genes by CRISPRa elicits potent anti-tumor immunity
por: Wang, Guangchuan, et al.
Publicado: (2019)

Maybe you can turn me on: CRISPRa-based strategies for therapeutic applications
por: Becirovic, Elvir
Publicado: (2022)

Parkinson's disease motor symptoms rescue by CRISPRa‐reprogramming astrocytes into GABAergic neurons
por: Giehrl‐Schwab, Jessica, et al.
Publicado: (2022)

Different transcriptional responses by the CRISPRa system in distinct types of heterochromatin in Drosophila melanogaster
por: Ortega-Yáñez, Andrea, et al.
Publicado: (2022)

Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9
por: Ye, Lupeng, et al.
Publicado: (2018)

Multistable and dynamic CRISPRi-based synthetic circuits
por: Santos-Moreno, Javier, et al.
Publicado: (2020)

Transcriptional control of Clostridium autoethanogenum using CRISPRi
por: Fackler, Nicholas, et al.
Publicado: (2021)

CRISPRi-Library-Guided Target Identification for Engineering Carotenoid Production by Corynebacterium glutamicum
por: Göttl, Vanessa L., et al.
Publicado: (2021)

In vivo CRISPRa decreases seizures and rescues cognitive deficits in a rodent model of epilepsy
por: Colasante, Gaia, et al.
Publicado: (2020)

In silico performance analysis of web tools for CRISPRa sgRNA design in human genes
por: Nuñez Pedrozo, Cristian N., et al.
Publicado: (2022)

Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma
por: Sgro, Agustin, et al.
Publicado: (2023)

Phase-separated DropCRISPRa platform for efficient gene activation in mammalian cells and mice
por: Ma, Shufeng, et al.
Publicado: (2023)

Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library
por: Miyamoto, Naoki, et al.
Publicado: (2021)

Development of an integrated CRISPRi targeting ΔNp63 for treatment of squamous cell carcinoma
por: Yoshida, Masakazu, et al.
Publicado: (2018)

Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)
por: Cleto, Sara, et al.
Publicado: (2016)

Enabling Genetic Analysis of Diverse Bacteria with Mobile-CRISPRi
por: Peters, Jason M., et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_6fkemmde8k6fikkgqthd9q4fbo