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Genome–Transcriptome Transition Approaches to Characterize Anthocyanin Biosynthesis Pathway Genes in Blue, Black and Purple Wheat
Colored wheat has gained enormous attention from the scientific community, but the information available on the anthocyanin biosynthetic genes is very minimal. The study involved their genome-wide identification, in silico characterization and differential expression analysis among purple, blue, bla...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137985/ https://www.ncbi.nlm.nih.gov/pubmed/37107567 http://dx.doi.org/10.3390/genes14040809 |
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author | Kapoor, Payal Sharma, Saloni Tiwari, Apoorv Kaur, Satveer Kumari, Anita Sonah, Humira Goyal, Ajay Krishania, Meena Garg, Monika |
author_facet | Kapoor, Payal Sharma, Saloni Tiwari, Apoorv Kaur, Satveer Kumari, Anita Sonah, Humira Goyal, Ajay Krishania, Meena Garg, Monika |
author_sort | Kapoor, Payal |
collection | PubMed |
description | Colored wheat has gained enormous attention from the scientific community, but the information available on the anthocyanin biosynthetic genes is very minimal. The study involved their genome-wide identification, in silico characterization and differential expression analysis among purple, blue, black and white wheat lines. The recently released wheat genome mining putatively identified eight structural genes in the anthocyanin biosynthesis pathway with a total of 1194 isoforms. Genes showed distinct exon architecture, domain profile, regulatory elements, chromosome emplacement, tissue localization, phylogeny and synteny, indicative of their unique function. RNA sequencing of developing seeds from colored (black, blue and purple) and white wheats identified differential expressions in 97 isoforms. The F3H on group two chromosomes and F3′5′H on 1D chromosomes could be significant influencers in purple and blue color development, respectively. Apart from a role in anthocyanin biosynthesis, these putative structural genes also played an important role in light, drought, low temperature and other defense responses. The information can assist in targeted anthocyanin production in the wheat seed endosperm. |
format | Online Article Text |
id | pubmed-10137985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101379852023-04-28 Genome–Transcriptome Transition Approaches to Characterize Anthocyanin Biosynthesis Pathway Genes in Blue, Black and Purple Wheat Kapoor, Payal Sharma, Saloni Tiwari, Apoorv Kaur, Satveer Kumari, Anita Sonah, Humira Goyal, Ajay Krishania, Meena Garg, Monika Genes (Basel) Article Colored wheat has gained enormous attention from the scientific community, but the information available on the anthocyanin biosynthetic genes is very minimal. The study involved their genome-wide identification, in silico characterization and differential expression analysis among purple, blue, black and white wheat lines. The recently released wheat genome mining putatively identified eight structural genes in the anthocyanin biosynthesis pathway with a total of 1194 isoforms. Genes showed distinct exon architecture, domain profile, regulatory elements, chromosome emplacement, tissue localization, phylogeny and synteny, indicative of their unique function. RNA sequencing of developing seeds from colored (black, blue and purple) and white wheats identified differential expressions in 97 isoforms. The F3H on group two chromosomes and F3′5′H on 1D chromosomes could be significant influencers in purple and blue color development, respectively. Apart from a role in anthocyanin biosynthesis, these putative structural genes also played an important role in light, drought, low temperature and other defense responses. The information can assist in targeted anthocyanin production in the wheat seed endosperm. MDPI 2023-03-27 /pmc/articles/PMC10137985/ /pubmed/37107567 http://dx.doi.org/10.3390/genes14040809 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kapoor, Payal Sharma, Saloni Tiwari, Apoorv Kaur, Satveer Kumari, Anita Sonah, Humira Goyal, Ajay Krishania, Meena Garg, Monika Genome–Transcriptome Transition Approaches to Characterize Anthocyanin Biosynthesis Pathway Genes in Blue, Black and Purple Wheat |
title | Genome–Transcriptome Transition Approaches to Characterize Anthocyanin Biosynthesis Pathway Genes in Blue, Black and Purple Wheat |
title_full | Genome–Transcriptome Transition Approaches to Characterize Anthocyanin Biosynthesis Pathway Genes in Blue, Black and Purple Wheat |
title_fullStr | Genome–Transcriptome Transition Approaches to Characterize Anthocyanin Biosynthesis Pathway Genes in Blue, Black and Purple Wheat |
title_full_unstemmed | Genome–Transcriptome Transition Approaches to Characterize Anthocyanin Biosynthesis Pathway Genes in Blue, Black and Purple Wheat |
title_short | Genome–Transcriptome Transition Approaches to Characterize Anthocyanin Biosynthesis Pathway Genes in Blue, Black and Purple Wheat |
title_sort | genome–transcriptome transition approaches to characterize anthocyanin biosynthesis pathway genes in blue, black and purple wheat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137985/ https://www.ncbi.nlm.nih.gov/pubmed/37107567 http://dx.doi.org/10.3390/genes14040809 |
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