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Evaluation of alcohol-free mouthwash for studies of the oral microbiome
Oral bacteria play important roles in human health and disease. Oral samples collected using ethanol-containing mouthwash are widely used for oral microbiome studies. However, ethanol is flammable and not ideal for transportation/storage in large quantities, and some individuals may avoid ethanol du...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138257/ https://www.ncbi.nlm.nih.gov/pubmed/37104300 http://dx.doi.org/10.1371/journal.pone.0284956 |
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author | Yano, Yukiko Vogtmann, Emily Shreves, Alaina H. Weinstein, Stephanie J. Black, Amanda Diaz-Mayoral, Norma Wan, Yunhu Zhou, Weiyin Hua, Xing Dagnall, Casey L. Hutchinson, Amy Jones, Kristine Hicks, Belynda D. Wyatt, Kathleen Brotzman, Michelle Gerlanc, Nicole Huang, Wen-Yi Albert, Paul S. Wentzensen, Nicolas Abnet, Christian C. |
author_facet | Yano, Yukiko Vogtmann, Emily Shreves, Alaina H. Weinstein, Stephanie J. Black, Amanda Diaz-Mayoral, Norma Wan, Yunhu Zhou, Weiyin Hua, Xing Dagnall, Casey L. Hutchinson, Amy Jones, Kristine Hicks, Belynda D. Wyatt, Kathleen Brotzman, Michelle Gerlanc, Nicole Huang, Wen-Yi Albert, Paul S. Wentzensen, Nicolas Abnet, Christian C. |
author_sort | Yano, Yukiko |
collection | PubMed |
description | Oral bacteria play important roles in human health and disease. Oral samples collected using ethanol-containing mouthwash are widely used for oral microbiome studies. However, ethanol is flammable and not ideal for transportation/storage in large quantities, and some individuals may avoid ethanol due to the burning sensation or due to various personal, medical, religious, and/or cultural factors. Here, we compared ethanol-free and ethanol-containing mouthwashes using multiple microbiome metrics and assessed the stability of the mouthwash samples stored up to 10 days before processing. Forty volunteers provided oral wash samples collected using ethanol-free and ethanol-containing mouthwashes. From each sample, one aliquot was immediately frozen, one was stored at 4°C for 5 days and frozen, while the third aliquot was stored for 5 days at 4°C and 5 days at ambient temperature to mimic shipping delays and then frozen. DNA was extracted, the 16S rRNA gene V4 region was amplified and sequenced, and bioinformatic processing was performed using QIIME 2. Microbiome metrics measured in the two mouthwash types were very similar, with intraclass correlation coefficients (ICCs) for alpha and beta diversity metrics greater than 0.85. Relative abundances of some taxa were significantly different, but ICCs of the top four most abundant phyla and genera were high (> 0.75) for the comparability of the mouthwashes. Stability during delayed processing was also high for both mouthwashes based on alpha and beta diversity measures and relative abundances of the top four phyla and genera (ICCs ≥ 0.90). These results demonstrate ethanol-free mouthwash performs similarly to ethanol-containing mouthwash for microbial analyses, and both mouthwashes are stable for at least 10 days without freezing prior to laboratory processing. Ethanol-free mouthwash is suitable for collecting and shipping oral wash samples, and these results have important implications for planning future epidemiologic studies of the oral microbiome. |
format | Online Article Text |
id | pubmed-10138257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-101382572023-04-28 Evaluation of alcohol-free mouthwash for studies of the oral microbiome Yano, Yukiko Vogtmann, Emily Shreves, Alaina H. Weinstein, Stephanie J. Black, Amanda Diaz-Mayoral, Norma Wan, Yunhu Zhou, Weiyin Hua, Xing Dagnall, Casey L. Hutchinson, Amy Jones, Kristine Hicks, Belynda D. Wyatt, Kathleen Brotzman, Michelle Gerlanc, Nicole Huang, Wen-Yi Albert, Paul S. Wentzensen, Nicolas Abnet, Christian C. PLoS One Research Article Oral bacteria play important roles in human health and disease. Oral samples collected using ethanol-containing mouthwash are widely used for oral microbiome studies. However, ethanol is flammable and not ideal for transportation/storage in large quantities, and some individuals may avoid ethanol due to the burning sensation or due to various personal, medical, religious, and/or cultural factors. Here, we compared ethanol-free and ethanol-containing mouthwashes using multiple microbiome metrics and assessed the stability of the mouthwash samples stored up to 10 days before processing. Forty volunteers provided oral wash samples collected using ethanol-free and ethanol-containing mouthwashes. From each sample, one aliquot was immediately frozen, one was stored at 4°C for 5 days and frozen, while the third aliquot was stored for 5 days at 4°C and 5 days at ambient temperature to mimic shipping delays and then frozen. DNA was extracted, the 16S rRNA gene V4 region was amplified and sequenced, and bioinformatic processing was performed using QIIME 2. Microbiome metrics measured in the two mouthwash types were very similar, with intraclass correlation coefficients (ICCs) for alpha and beta diversity metrics greater than 0.85. Relative abundances of some taxa were significantly different, but ICCs of the top four most abundant phyla and genera were high (> 0.75) for the comparability of the mouthwashes. Stability during delayed processing was also high for both mouthwashes based on alpha and beta diversity measures and relative abundances of the top four phyla and genera (ICCs ≥ 0.90). These results demonstrate ethanol-free mouthwash performs similarly to ethanol-containing mouthwash for microbial analyses, and both mouthwashes are stable for at least 10 days without freezing prior to laboratory processing. Ethanol-free mouthwash is suitable for collecting and shipping oral wash samples, and these results have important implications for planning future epidemiologic studies of the oral microbiome. Public Library of Science 2023-04-27 /pmc/articles/PMC10138257/ /pubmed/37104300 http://dx.doi.org/10.1371/journal.pone.0284956 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Yano, Yukiko Vogtmann, Emily Shreves, Alaina H. Weinstein, Stephanie J. Black, Amanda Diaz-Mayoral, Norma Wan, Yunhu Zhou, Weiyin Hua, Xing Dagnall, Casey L. Hutchinson, Amy Jones, Kristine Hicks, Belynda D. Wyatt, Kathleen Brotzman, Michelle Gerlanc, Nicole Huang, Wen-Yi Albert, Paul S. Wentzensen, Nicolas Abnet, Christian C. Evaluation of alcohol-free mouthwash for studies of the oral microbiome |
title | Evaluation of alcohol-free mouthwash for studies of the oral microbiome |
title_full | Evaluation of alcohol-free mouthwash for studies of the oral microbiome |
title_fullStr | Evaluation of alcohol-free mouthwash for studies of the oral microbiome |
title_full_unstemmed | Evaluation of alcohol-free mouthwash for studies of the oral microbiome |
title_short | Evaluation of alcohol-free mouthwash for studies of the oral microbiome |
title_sort | evaluation of alcohol-free mouthwash for studies of the oral microbiome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138257/ https://www.ncbi.nlm.nih.gov/pubmed/37104300 http://dx.doi.org/10.1371/journal.pone.0284956 |
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