Cargando…

Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture

One approach to cell expansion is to use large hydrogel for growing a large number of cells. Nanofibrillar cellulose (NFC) hydrogel has been used for human induced pluripotent stem cell (hiPSCs) expansion. However, little is known about the status of hiPSCs at the single cell level inside large NFC...

Descripción completa

Detalles Bibliográficos
Autores principales: Hao, Jin, Chen, Ying, Zhu, Mingjian, Zhao, Yingqing, Zhang, Kai, Xu, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138276/
https://www.ncbi.nlm.nih.gov/pubmed/37102936
http://dx.doi.org/10.3390/gels9040324
_version_ 1785032668743729152
author Hao, Jin
Chen, Ying
Zhu, Mingjian
Zhao, Yingqing
Zhang, Kai
Xu, Xia
author_facet Hao, Jin
Chen, Ying
Zhu, Mingjian
Zhao, Yingqing
Zhang, Kai
Xu, Xia
author_sort Hao, Jin
collection PubMed
description One approach to cell expansion is to use large hydrogel for growing a large number of cells. Nanofibrillar cellulose (NFC) hydrogel has been used for human induced pluripotent stem cell (hiPSCs) expansion. However, little is known about the status of hiPSCs at the single cell level inside large NFC hydrogel during culture. To understand the effect of NFC hydrogel property on temporal–spatial heterogeneity, hiPSCs were cultured in 0.8 wt% NFC hydrogel with different thicknesses with the top surface exposed to the culture medium. The prepared hydrogel exhibits less restriction in mass transfer due to the presence of macropores and micropores interconnecting the macropores. More than 85% of cells at different depths survive after 5 days of culture inside 3.5 mm thick hydrogel. Biological compositions at different zones inside the NFC gel were examined over time at a single-cell level. A dramatic concentration gradient of growth factors estimated in the simulation along 3.5 mm NFC hydrogel could be a reason for the spatial–temporal heterogeneity in protein secondary structure and protein glycosylation and pluripotency loss at the bottom zone. pH change caused by the lactic acid accumulation over time leads to changes in cellulose charge and growth factor potential, probably another reason for the heterogeneity in biochemical compositions. This study may help to develop optimal conditions for producing high-quality hiPSCs in large nanofibrillar cellulose hydrogel at scale.
format Online
Article
Text
id pubmed-10138276
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101382762023-04-28 Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture Hao, Jin Chen, Ying Zhu, Mingjian Zhao, Yingqing Zhang, Kai Xu, Xia Gels Article One approach to cell expansion is to use large hydrogel for growing a large number of cells. Nanofibrillar cellulose (NFC) hydrogel has been used for human induced pluripotent stem cell (hiPSCs) expansion. However, little is known about the status of hiPSCs at the single cell level inside large NFC hydrogel during culture. To understand the effect of NFC hydrogel property on temporal–spatial heterogeneity, hiPSCs were cultured in 0.8 wt% NFC hydrogel with different thicknesses with the top surface exposed to the culture medium. The prepared hydrogel exhibits less restriction in mass transfer due to the presence of macropores and micropores interconnecting the macropores. More than 85% of cells at different depths survive after 5 days of culture inside 3.5 mm thick hydrogel. Biological compositions at different zones inside the NFC gel were examined over time at a single-cell level. A dramatic concentration gradient of growth factors estimated in the simulation along 3.5 mm NFC hydrogel could be a reason for the spatial–temporal heterogeneity in protein secondary structure and protein glycosylation and pluripotency loss at the bottom zone. pH change caused by the lactic acid accumulation over time leads to changes in cellulose charge and growth factor potential, probably another reason for the heterogeneity in biochemical compositions. This study may help to develop optimal conditions for producing high-quality hiPSCs in large nanofibrillar cellulose hydrogel at scale. MDPI 2023-04-12 /pmc/articles/PMC10138276/ /pubmed/37102936 http://dx.doi.org/10.3390/gels9040324 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hao, Jin
Chen, Ying
Zhu, Mingjian
Zhao, Yingqing
Zhang, Kai
Xu, Xia
Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture
title Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture
title_full Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture
title_fullStr Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture
title_full_unstemmed Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture
title_short Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture
title_sort spatial-temporal heterogeneity in large three-dimensional nanofibrillar cellulose hydrogel for human pluripotent stem cell culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138276/
https://www.ncbi.nlm.nih.gov/pubmed/37102936
http://dx.doi.org/10.3390/gels9040324
work_keys_str_mv AT haojin spatialtemporalheterogeneityinlargethreedimensionalnanofibrillarcellulosehydrogelforhumanpluripotentstemcellculture
AT chenying spatialtemporalheterogeneityinlargethreedimensionalnanofibrillarcellulosehydrogelforhumanpluripotentstemcellculture
AT zhumingjian spatialtemporalheterogeneityinlargethreedimensionalnanofibrillarcellulosehydrogelforhumanpluripotentstemcellculture
AT zhaoyingqing spatialtemporalheterogeneityinlargethreedimensionalnanofibrillarcellulosehydrogelforhumanpluripotentstemcellculture
AT zhangkai spatialtemporalheterogeneityinlargethreedimensionalnanofibrillarcellulosehydrogelforhumanpluripotentstemcellculture
AT xuxia spatialtemporalheterogeneityinlargethreedimensionalnanofibrillarcellulosehydrogelforhumanpluripotentstemcellculture