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Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes

Membrane proteins play an important role in key cellular functions, such as signal transduction, apoptosis, and metabolism. Therefore, structural and functional studies of these proteins are essential in fields such as fundamental biology, medical science, pharmacology, biotechnology, and bioenginee...

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Detalles Bibliográficos
Autores principales: Tosaka, Toshiyuki, Kamiya, Koki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138308/
https://www.ncbi.nlm.nih.gov/pubmed/37108393
http://dx.doi.org/10.3390/ijms24087231
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author Tosaka, Toshiyuki
Kamiya, Koki
author_facet Tosaka, Toshiyuki
Kamiya, Koki
author_sort Tosaka, Toshiyuki
collection PubMed
description Membrane proteins play an important role in key cellular functions, such as signal transduction, apoptosis, and metabolism. Therefore, structural and functional studies of these proteins are essential in fields such as fundamental biology, medical science, pharmacology, biotechnology, and bioengineering. However, observing the precise elemental reactions and structures of membrane proteins is difficult, despite their functioning through interactions with various biomolecules in living cells. To investigate these properties, methodologies have been developed to study the functions of membrane proteins that have been purified from biological cells. In this paper, we introduce various methods for creating liposomes or lipid vesicles, from conventional to recent approaches, as well as techniques for reconstituting membrane proteins into artificial membranes. We also cover the different types of artificial membranes that can be used to observe the functions of reconstituted membrane proteins, including their structure, number of transmembrane domains, and functional type. Finally, we discuss the reconstitution of membrane proteins using a cell-free synthesis system and the reconstitution and function of multiple membrane proteins.
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spelling pubmed-101383082023-04-28 Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes Tosaka, Toshiyuki Kamiya, Koki Int J Mol Sci Review Membrane proteins play an important role in key cellular functions, such as signal transduction, apoptosis, and metabolism. Therefore, structural and functional studies of these proteins are essential in fields such as fundamental biology, medical science, pharmacology, biotechnology, and bioengineering. However, observing the precise elemental reactions and structures of membrane proteins is difficult, despite their functioning through interactions with various biomolecules in living cells. To investigate these properties, methodologies have been developed to study the functions of membrane proteins that have been purified from biological cells. In this paper, we introduce various methods for creating liposomes or lipid vesicles, from conventional to recent approaches, as well as techniques for reconstituting membrane proteins into artificial membranes. We also cover the different types of artificial membranes that can be used to observe the functions of reconstituted membrane proteins, including their structure, number of transmembrane domains, and functional type. Finally, we discuss the reconstitution of membrane proteins using a cell-free synthesis system and the reconstitution and function of multiple membrane proteins. MDPI 2023-04-13 /pmc/articles/PMC10138308/ /pubmed/37108393 http://dx.doi.org/10.3390/ijms24087231 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Tosaka, Toshiyuki
Kamiya, Koki
Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes
title Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes
title_full Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes
title_fullStr Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes
title_full_unstemmed Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes
title_short Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes
title_sort function investigations and applications of membrane proteins on artificial lipid membranes
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138308/
https://www.ncbi.nlm.nih.gov/pubmed/37108393
http://dx.doi.org/10.3390/ijms24087231
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