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Magnoflorine from Berberis vulgaris Roots—Impact on Hippocampal Neurons in Mice after Short-Term Exposure

In search of novel potential drug candidates that could be used as treatments or prophylactics for memory impairment, an aporphine alkaloid magnoflorine (MAG) isolated from the root of Berberis vulgaris was proven to exhibit beneficial anti-amnestic properties. Its effects on immunoreactivity to par...

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Autores principales: Szalak, Radosław, Matysek, Małgorzata, Koval, Maryna, Dziedzic, Marcin, Kowalczuk-Vasilev, Edyta, Kruk-Slomka, Marta, Koch, Wojciech, Arciszewski, Marcin B., Kukula-Koch, Wirginia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138352/
https://www.ncbi.nlm.nih.gov/pubmed/37108329
http://dx.doi.org/10.3390/ijms24087166
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author Szalak, Radosław
Matysek, Małgorzata
Koval, Maryna
Dziedzic, Marcin
Kowalczuk-Vasilev, Edyta
Kruk-Slomka, Marta
Koch, Wojciech
Arciszewski, Marcin B.
Kukula-Koch, Wirginia
author_facet Szalak, Radosław
Matysek, Małgorzata
Koval, Maryna
Dziedzic, Marcin
Kowalczuk-Vasilev, Edyta
Kruk-Slomka, Marta
Koch, Wojciech
Arciszewski, Marcin B.
Kukula-Koch, Wirginia
author_sort Szalak, Radosław
collection PubMed
description In search of novel potential drug candidates that could be used as treatments or prophylactics for memory impairment, an aporphine alkaloid magnoflorine (MAG) isolated from the root of Berberis vulgaris was proven to exhibit beneficial anti-amnestic properties. Its effects on immunoreactivity to parvalbumin in the mouse hippocampus were assessed together with a study on its safety and concentration in the brain and plasma. For this purpose, four experimental groups were created: the MAG10 group—treated with 10 mg MAG/kg b.w. i.p., the MAG20 group—treated with 20 mg MAG/kg b.w. i.p., the MAG50 group—treated with 50 mg MAG/kg b.w. i.p., and a control group—injected with saline i.p. at a volume corresponding to their weight. Our results indicated that the hippocampal fields CA1–CA3 were characterized by an elevated number of parvalbumin-immunoreactive neurons (PV-IR) and nerve fibers in mice at the doses of 10 and 20 mg/kg b.w. (i.p.). No significant changes to the levels of IL-1β, IL-6 or TNF-α were observed for the above two doses; however, the administration of 50 mg/kg b.w. i.p. caused a statistically significant elevation of IL-6, IL-1beta plasma levels and an insignificant raise in the TNF-alpha value. The HPLC–MS analysis showed that the alkaloid’s content in the brain structures in the group treated with 50 mg/kg b.w. did not increase proportionally with the administered dose. The obtained results show that MAG is able to influence the immunoreactivity to PV-IR in hippocampal neurons and might act as a neuroprotective compound.
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spelling pubmed-101383522023-04-28 Magnoflorine from Berberis vulgaris Roots—Impact on Hippocampal Neurons in Mice after Short-Term Exposure Szalak, Radosław Matysek, Małgorzata Koval, Maryna Dziedzic, Marcin Kowalczuk-Vasilev, Edyta Kruk-Slomka, Marta Koch, Wojciech Arciszewski, Marcin B. Kukula-Koch, Wirginia Int J Mol Sci Article In search of novel potential drug candidates that could be used as treatments or prophylactics for memory impairment, an aporphine alkaloid magnoflorine (MAG) isolated from the root of Berberis vulgaris was proven to exhibit beneficial anti-amnestic properties. Its effects on immunoreactivity to parvalbumin in the mouse hippocampus were assessed together with a study on its safety and concentration in the brain and plasma. For this purpose, four experimental groups were created: the MAG10 group—treated with 10 mg MAG/kg b.w. i.p., the MAG20 group—treated with 20 mg MAG/kg b.w. i.p., the MAG50 group—treated with 50 mg MAG/kg b.w. i.p., and a control group—injected with saline i.p. at a volume corresponding to their weight. Our results indicated that the hippocampal fields CA1–CA3 were characterized by an elevated number of parvalbumin-immunoreactive neurons (PV-IR) and nerve fibers in mice at the doses of 10 and 20 mg/kg b.w. (i.p.). No significant changes to the levels of IL-1β, IL-6 or TNF-α were observed for the above two doses; however, the administration of 50 mg/kg b.w. i.p. caused a statistically significant elevation of IL-6, IL-1beta plasma levels and an insignificant raise in the TNF-alpha value. The HPLC–MS analysis showed that the alkaloid’s content in the brain structures in the group treated with 50 mg/kg b.w. did not increase proportionally with the administered dose. The obtained results show that MAG is able to influence the immunoreactivity to PV-IR in hippocampal neurons and might act as a neuroprotective compound. MDPI 2023-04-12 /pmc/articles/PMC10138352/ /pubmed/37108329 http://dx.doi.org/10.3390/ijms24087166 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szalak, Radosław
Matysek, Małgorzata
Koval, Maryna
Dziedzic, Marcin
Kowalczuk-Vasilev, Edyta
Kruk-Slomka, Marta
Koch, Wojciech
Arciszewski, Marcin B.
Kukula-Koch, Wirginia
Magnoflorine from Berberis vulgaris Roots—Impact on Hippocampal Neurons in Mice after Short-Term Exposure
title Magnoflorine from Berberis vulgaris Roots—Impact on Hippocampal Neurons in Mice after Short-Term Exposure
title_full Magnoflorine from Berberis vulgaris Roots—Impact on Hippocampal Neurons in Mice after Short-Term Exposure
title_fullStr Magnoflorine from Berberis vulgaris Roots—Impact on Hippocampal Neurons in Mice after Short-Term Exposure
title_full_unstemmed Magnoflorine from Berberis vulgaris Roots—Impact on Hippocampal Neurons in Mice after Short-Term Exposure
title_short Magnoflorine from Berberis vulgaris Roots—Impact on Hippocampal Neurons in Mice after Short-Term Exposure
title_sort magnoflorine from berberis vulgaris roots—impact on hippocampal neurons in mice after short-term exposure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138352/
https://www.ncbi.nlm.nih.gov/pubmed/37108329
http://dx.doi.org/10.3390/ijms24087166
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