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Exploring the Metabolic Differences between Cisplatin- and UV Light-Induced Apoptotic Bodies in HK-2 Cells by an Untargeted Metabolomics Approach

Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during the apoptosis and perform a relevant role in the pathogenesis of different diseases. Recently, it has been demonstrated that ABs from human renal proximal tubular HK-2 cells, either induced by cisplatin or by UV light, c...

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Autores principales: Bernardo-Bermejo, Samuel, Sánchez-López, Elena, Castro-Puyana, María, Fernández-Martínez, Ana B., Lucio-Cazaña, Francisco Javier, Marina, María Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138416/
https://www.ncbi.nlm.nih.gov/pubmed/37108400
http://dx.doi.org/10.3390/ijms24087237
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author Bernardo-Bermejo, Samuel
Sánchez-López, Elena
Castro-Puyana, María
Fernández-Martínez, Ana B.
Lucio-Cazaña, Francisco Javier
Marina, María Luisa
author_facet Bernardo-Bermejo, Samuel
Sánchez-López, Elena
Castro-Puyana, María
Fernández-Martínez, Ana B.
Lucio-Cazaña, Francisco Javier
Marina, María Luisa
author_sort Bernardo-Bermejo, Samuel
collection PubMed
description Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during the apoptosis and perform a relevant role in the pathogenesis of different diseases. Recently, it has been demonstrated that ABs from human renal proximal tubular HK-2 cells, either induced by cisplatin or by UV light, can lead to further apoptotic death in naïve HK-2 cells. Thus, the aim of this work was to carry out a non-targeted metabolomic approach to study if the apoptotic stimulus (cisplatin or UV light) affects in a different way the metabolites involved in the propagation of apoptosis. Both ABs and their extracellular fluid were analyzed using a reverse-phase liquid chromatography-mass spectrometry setup. Principal components analysis showed a tight clustering of each experimental group and partial least square discriminant analysis was used to assess the metabolic differences existing between these groups. Considering the variable importance in the projection values, molecular features were selected and some of them could be identified either unequivocally or tentatively. The resulting pathways indicated that there are significant, stimulus-specific differences in metabolites abundancies that may propagate apoptosis to healthy proximal tubular cells; thus, we hypothesize that the share in apoptosis of these metabolites might vary depending on the apoptotic stimulus.
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spelling pubmed-101384162023-04-28 Exploring the Metabolic Differences between Cisplatin- and UV Light-Induced Apoptotic Bodies in HK-2 Cells by an Untargeted Metabolomics Approach Bernardo-Bermejo, Samuel Sánchez-López, Elena Castro-Puyana, María Fernández-Martínez, Ana B. Lucio-Cazaña, Francisco Javier Marina, María Luisa Int J Mol Sci Article Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during the apoptosis and perform a relevant role in the pathogenesis of different diseases. Recently, it has been demonstrated that ABs from human renal proximal tubular HK-2 cells, either induced by cisplatin or by UV light, can lead to further apoptotic death in naïve HK-2 cells. Thus, the aim of this work was to carry out a non-targeted metabolomic approach to study if the apoptotic stimulus (cisplatin or UV light) affects in a different way the metabolites involved in the propagation of apoptosis. Both ABs and their extracellular fluid were analyzed using a reverse-phase liquid chromatography-mass spectrometry setup. Principal components analysis showed a tight clustering of each experimental group and partial least square discriminant analysis was used to assess the metabolic differences existing between these groups. Considering the variable importance in the projection values, molecular features were selected and some of them could be identified either unequivocally or tentatively. The resulting pathways indicated that there are significant, stimulus-specific differences in metabolites abundancies that may propagate apoptosis to healthy proximal tubular cells; thus, we hypothesize that the share in apoptosis of these metabolites might vary depending on the apoptotic stimulus. MDPI 2023-04-14 /pmc/articles/PMC10138416/ /pubmed/37108400 http://dx.doi.org/10.3390/ijms24087237 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bernardo-Bermejo, Samuel
Sánchez-López, Elena
Castro-Puyana, María
Fernández-Martínez, Ana B.
Lucio-Cazaña, Francisco Javier
Marina, María Luisa
Exploring the Metabolic Differences between Cisplatin- and UV Light-Induced Apoptotic Bodies in HK-2 Cells by an Untargeted Metabolomics Approach
title Exploring the Metabolic Differences between Cisplatin- and UV Light-Induced Apoptotic Bodies in HK-2 Cells by an Untargeted Metabolomics Approach
title_full Exploring the Metabolic Differences between Cisplatin- and UV Light-Induced Apoptotic Bodies in HK-2 Cells by an Untargeted Metabolomics Approach
title_fullStr Exploring the Metabolic Differences between Cisplatin- and UV Light-Induced Apoptotic Bodies in HK-2 Cells by an Untargeted Metabolomics Approach
title_full_unstemmed Exploring the Metabolic Differences between Cisplatin- and UV Light-Induced Apoptotic Bodies in HK-2 Cells by an Untargeted Metabolomics Approach
title_short Exploring the Metabolic Differences between Cisplatin- and UV Light-Induced Apoptotic Bodies in HK-2 Cells by an Untargeted Metabolomics Approach
title_sort exploring the metabolic differences between cisplatin- and uv light-induced apoptotic bodies in hk-2 cells by an untargeted metabolomics approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138416/
https://www.ncbi.nlm.nih.gov/pubmed/37108400
http://dx.doi.org/10.3390/ijms24087237
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