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TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

Natural pyrethrins have high application value, and are widely used as a green pesticide in crop pest prevention and control. Pyrethrins are mainly extracted from the flower heads of Tanacetum cinerariifolium; however, the natural content is low. Therefore, it is essential to understand the regulato...

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Autores principales: Zeng, Tuo, Yu, Qin, Shang, Junzhong, Xu, Zhizhuo, Zhou, Li, Li, Wei, Li, Jinjin, Hu, Hao, Zhu, Liyong, Li, Jiawen, Wang, Caiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138541/
https://www.ncbi.nlm.nih.gov/pubmed/37108541
http://dx.doi.org/10.3390/ijms24087379
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author Zeng, Tuo
Yu, Qin
Shang, Junzhong
Xu, Zhizhuo
Zhou, Li
Li, Wei
Li, Jinjin
Hu, Hao
Zhu, Liyong
Li, Jiawen
Wang, Caiyun
author_facet Zeng, Tuo
Yu, Qin
Shang, Junzhong
Xu, Zhizhuo
Zhou, Li
Li, Wei
Li, Jinjin
Hu, Hao
Zhu, Liyong
Li, Jiawen
Wang, Caiyun
author_sort Zeng, Tuo
collection PubMed
description Natural pyrethrins have high application value, and are widely used as a green pesticide in crop pest prevention and control. Pyrethrins are mainly extracted from the flower heads of Tanacetum cinerariifolium; however, the natural content is low. Therefore, it is essential to understand the regulatory mechanisms underlying the synthesis of pyrethrins through identification of key transcription factors. We identified a gene encoding a MYC2-like transcription factor named TcbHLH14 from T. cinerariifolium transcriptome, which is induced by methyl jasmonate. In the present study, we evaluated the regulatory effects and mechanisms of TcbHLH14 using expression analysis, a yeast one-hybrid assay, electrophoretic mobility shift assay, and overexpression/virus-induced gene silencing experiments. We found that TcbHLH14 can directly bind to the cis-elements of the pyrethrins synthesis genes TcAOC and TcGLIP to activate their expression. The transient overexpression of TcbHLH14 enhanced expression of the TcAOC and TcGLIP genes. Conversely, transient silencing of TcbHLH14 downregulated the expression of TcAOC and TcGLIP and reduced the content of pyrethrins. In summary, these results indicate that the potential application of TcbHLH14 in improving the germplasm resources and provide a new insight into the regulatory network of pyrethrins biosynthesis of T. cinerariifolium to further inform the development of engineering strategies for increasing pyrethrins contents.
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spelling pubmed-101385412023-04-28 TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium Zeng, Tuo Yu, Qin Shang, Junzhong Xu, Zhizhuo Zhou, Li Li, Wei Li, Jinjin Hu, Hao Zhu, Liyong Li, Jiawen Wang, Caiyun Int J Mol Sci Article Natural pyrethrins have high application value, and are widely used as a green pesticide in crop pest prevention and control. Pyrethrins are mainly extracted from the flower heads of Tanacetum cinerariifolium; however, the natural content is low. Therefore, it is essential to understand the regulatory mechanisms underlying the synthesis of pyrethrins through identification of key transcription factors. We identified a gene encoding a MYC2-like transcription factor named TcbHLH14 from T. cinerariifolium transcriptome, which is induced by methyl jasmonate. In the present study, we evaluated the regulatory effects and mechanisms of TcbHLH14 using expression analysis, a yeast one-hybrid assay, electrophoretic mobility shift assay, and overexpression/virus-induced gene silencing experiments. We found that TcbHLH14 can directly bind to the cis-elements of the pyrethrins synthesis genes TcAOC and TcGLIP to activate their expression. The transient overexpression of TcbHLH14 enhanced expression of the TcAOC and TcGLIP genes. Conversely, transient silencing of TcbHLH14 downregulated the expression of TcAOC and TcGLIP and reduced the content of pyrethrins. In summary, these results indicate that the potential application of TcbHLH14 in improving the germplasm resources and provide a new insight into the regulatory network of pyrethrins biosynthesis of T. cinerariifolium to further inform the development of engineering strategies for increasing pyrethrins contents. MDPI 2023-04-17 /pmc/articles/PMC10138541/ /pubmed/37108541 http://dx.doi.org/10.3390/ijms24087379 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zeng, Tuo
Yu, Qin
Shang, Junzhong
Xu, Zhizhuo
Zhou, Li
Li, Wei
Li, Jinjin
Hu, Hao
Zhu, Liyong
Li, Jiawen
Wang, Caiyun
TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium
title TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium
title_full TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium
title_fullStr TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium
title_full_unstemmed TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium
title_short TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium
title_sort tcbhlh14 a jasmonate associated myc2-like transcription factor positively regulates pyrethrin biosynthesis in tanacetum cinerariifolium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138541/
https://www.ncbi.nlm.nih.gov/pubmed/37108541
http://dx.doi.org/10.3390/ijms24087379
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