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Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and α-MSH for Skin Whitening

Pearl powder is a famous traditional Chinese medicine that has a long history in treating palpitations, insomnia, convulsions, epilepsy, ulcers, and skin lightining. Recently, several studies have demonstrated the effects of pearl extracts on protection of ultraviolet A (UVA) induced irritation on h...

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Autores principales: Yang, Shan, Wang, Zhekun, Hu, Yunwei, Zong, Kaile, Zhang, Xingjiang, Ke, Hui, Wang, Pan, Go, Yuyo, Chan, Xi Hui Felicia, Wu, Jianxin, Huang, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138581/
https://www.ncbi.nlm.nih.gov/pubmed/37108635
http://dx.doi.org/10.3390/ijms24087471
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author Yang, Shan
Wang, Zhekun
Hu, Yunwei
Zong, Kaile
Zhang, Xingjiang
Ke, Hui
Wang, Pan
Go, Yuyo
Chan, Xi Hui Felicia
Wu, Jianxin
Huang, Qing
author_facet Yang, Shan
Wang, Zhekun
Hu, Yunwei
Zong, Kaile
Zhang, Xingjiang
Ke, Hui
Wang, Pan
Go, Yuyo
Chan, Xi Hui Felicia
Wu, Jianxin
Huang, Qing
author_sort Yang, Shan
collection PubMed
description Pearl powder is a famous traditional Chinese medicine that has a long history in treating palpitations, insomnia, convulsions, epilepsy, ulcers, and skin lightining. Recently, several studies have demonstrated the effects of pearl extracts on protection of ultraviolet A (UVA) induced irritation on human skin fibroblasts and inhibition of melanin genesis on B16F10 mouse melanoma cells. To further explore the effect we focused on the whitening efficacy of pearl hydrolyzed conchiolin protein (HCP) on human melanoma MNT-1 cells under the irritation of alpha-melanocyte-stimulating hormone (α-MSH) or endothelin 1 (ET-1) to evaluate the intracellular tyrosinase and melanin contents, as well as the expression levels of tyrosinase (TYR), tyrosinase related protein 1 (TRP-1), and dopachrome tautomerase (DCT) genes and related proteins. We found that HCP could decrease the intracellular melanin content by reducing the activity of intracellular tyrosinase and inhibiting the expression of TYR, TRP-1, DCT genes and proteins. At the same time, the effect of HCP on melanosome transfer effect was also investigated in the co-culture system of immortalized human keratinocyte HaCaT cells with MNT-1. The result indicated that HCP could promote the transfer of melanosomes in MNT-1 melanocytes to HaCaT cells, which might accelerate the skin whitening process by quickly transferring and metabolizing melanosomes during keratinocyte differentiation. Further study is needed to explore the mechanism of melanosome transfer with depigmentation.
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spelling pubmed-101385812023-04-28 Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and α-MSH for Skin Whitening Yang, Shan Wang, Zhekun Hu, Yunwei Zong, Kaile Zhang, Xingjiang Ke, Hui Wang, Pan Go, Yuyo Chan, Xi Hui Felicia Wu, Jianxin Huang, Qing Int J Mol Sci Article Pearl powder is a famous traditional Chinese medicine that has a long history in treating palpitations, insomnia, convulsions, epilepsy, ulcers, and skin lightining. Recently, several studies have demonstrated the effects of pearl extracts on protection of ultraviolet A (UVA) induced irritation on human skin fibroblasts and inhibition of melanin genesis on B16F10 mouse melanoma cells. To further explore the effect we focused on the whitening efficacy of pearl hydrolyzed conchiolin protein (HCP) on human melanoma MNT-1 cells under the irritation of alpha-melanocyte-stimulating hormone (α-MSH) or endothelin 1 (ET-1) to evaluate the intracellular tyrosinase and melanin contents, as well as the expression levels of tyrosinase (TYR), tyrosinase related protein 1 (TRP-1), and dopachrome tautomerase (DCT) genes and related proteins. We found that HCP could decrease the intracellular melanin content by reducing the activity of intracellular tyrosinase and inhibiting the expression of TYR, TRP-1, DCT genes and proteins. At the same time, the effect of HCP on melanosome transfer effect was also investigated in the co-culture system of immortalized human keratinocyte HaCaT cells with MNT-1. The result indicated that HCP could promote the transfer of melanosomes in MNT-1 melanocytes to HaCaT cells, which might accelerate the skin whitening process by quickly transferring and metabolizing melanosomes during keratinocyte differentiation. Further study is needed to explore the mechanism of melanosome transfer with depigmentation. MDPI 2023-04-18 /pmc/articles/PMC10138581/ /pubmed/37108635 http://dx.doi.org/10.3390/ijms24087471 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Shan
Wang, Zhekun
Hu, Yunwei
Zong, Kaile
Zhang, Xingjiang
Ke, Hui
Wang, Pan
Go, Yuyo
Chan, Xi Hui Felicia
Wu, Jianxin
Huang, Qing
Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and α-MSH for Skin Whitening
title Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and α-MSH for Skin Whitening
title_full Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and α-MSH for Skin Whitening
title_fullStr Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and α-MSH for Skin Whitening
title_full_unstemmed Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and α-MSH for Skin Whitening
title_short Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and α-MSH for Skin Whitening
title_sort hydrolyzed conchiolin protein (hcp) extracted from pearls antagonizes both et-1 and α-msh for skin whitening
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138581/
https://www.ncbi.nlm.nih.gov/pubmed/37108635
http://dx.doi.org/10.3390/ijms24087471
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