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Notch and Wnt Signaling Modulation to Enhance DPSC Stemness and Therapeutic Potential

The Dental Pulp of permanent human teeth is home to stem cells with remarkable multilineage differentiation ability: human Dental Pulp Stem Cells (DPSCs). These cells display a very notorious expression of pluripotency core factors, and the ability to give rise to mature cell lineages belonging to t...

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Autores principales: Uribe-Etxebarria, Verónica, Pineda, Jose Ramon, García-Gallastegi, Patricia, Agliano, Alice, Unda, Fernando, Ibarretxe, Gaskon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138690/
https://www.ncbi.nlm.nih.gov/pubmed/37108549
http://dx.doi.org/10.3390/ijms24087389
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author Uribe-Etxebarria, Verónica
Pineda, Jose Ramon
García-Gallastegi, Patricia
Agliano, Alice
Unda, Fernando
Ibarretxe, Gaskon
author_facet Uribe-Etxebarria, Verónica
Pineda, Jose Ramon
García-Gallastegi, Patricia
Agliano, Alice
Unda, Fernando
Ibarretxe, Gaskon
author_sort Uribe-Etxebarria, Verónica
collection PubMed
description The Dental Pulp of permanent human teeth is home to stem cells with remarkable multilineage differentiation ability: human Dental Pulp Stem Cells (DPSCs). These cells display a very notorious expression of pluripotency core factors, and the ability to give rise to mature cell lineages belonging to the three embryonic layers. For these reasons, several researchers in the field have long considered human DPSCs as pluripotent-like cells. Notably, some signaling pathways such as Notch and Wnt contribute to maintaining the stemness of these cells through a complex network involving metabolic and epigenetic regulatory mechanisms. The use of recombinant proteins and selective pharmacological modulators of Notch and Wnt pathways, together with serum-free media and appropriate scaffolds that allow the maintenance of the non-differentiated state of hDPSC cultures could be an interesting approach to optimize the potency of these stem cells, without a need for genetic modification. In this review, we describe and integrate findings that shed light on the mechanisms responsible for stemness maintenance of hDPSCs, and how these are regulated by Notch/Wnt activation, drawing some interesting parallelisms with pluripotent stem cells. We summarize previous work on the stem cell field that includes interactions between epigenetics, metabolic regulations, and pluripotency core factor expression in hDPSCs and other stem cell types.
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spelling pubmed-101386902023-04-28 Notch and Wnt Signaling Modulation to Enhance DPSC Stemness and Therapeutic Potential Uribe-Etxebarria, Verónica Pineda, Jose Ramon García-Gallastegi, Patricia Agliano, Alice Unda, Fernando Ibarretxe, Gaskon Int J Mol Sci Review The Dental Pulp of permanent human teeth is home to stem cells with remarkable multilineage differentiation ability: human Dental Pulp Stem Cells (DPSCs). These cells display a very notorious expression of pluripotency core factors, and the ability to give rise to mature cell lineages belonging to the three embryonic layers. For these reasons, several researchers in the field have long considered human DPSCs as pluripotent-like cells. Notably, some signaling pathways such as Notch and Wnt contribute to maintaining the stemness of these cells through a complex network involving metabolic and epigenetic regulatory mechanisms. The use of recombinant proteins and selective pharmacological modulators of Notch and Wnt pathways, together with serum-free media and appropriate scaffolds that allow the maintenance of the non-differentiated state of hDPSC cultures could be an interesting approach to optimize the potency of these stem cells, without a need for genetic modification. In this review, we describe and integrate findings that shed light on the mechanisms responsible for stemness maintenance of hDPSCs, and how these are regulated by Notch/Wnt activation, drawing some interesting parallelisms with pluripotent stem cells. We summarize previous work on the stem cell field that includes interactions between epigenetics, metabolic regulations, and pluripotency core factor expression in hDPSCs and other stem cell types. MDPI 2023-04-17 /pmc/articles/PMC10138690/ /pubmed/37108549 http://dx.doi.org/10.3390/ijms24087389 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Uribe-Etxebarria, Verónica
Pineda, Jose Ramon
García-Gallastegi, Patricia
Agliano, Alice
Unda, Fernando
Ibarretxe, Gaskon
Notch and Wnt Signaling Modulation to Enhance DPSC Stemness and Therapeutic Potential
title Notch and Wnt Signaling Modulation to Enhance DPSC Stemness and Therapeutic Potential
title_full Notch and Wnt Signaling Modulation to Enhance DPSC Stemness and Therapeutic Potential
title_fullStr Notch and Wnt Signaling Modulation to Enhance DPSC Stemness and Therapeutic Potential
title_full_unstemmed Notch and Wnt Signaling Modulation to Enhance DPSC Stemness and Therapeutic Potential
title_short Notch and Wnt Signaling Modulation to Enhance DPSC Stemness and Therapeutic Potential
title_sort notch and wnt signaling modulation to enhance dpsc stemness and therapeutic potential
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138690/
https://www.ncbi.nlm.nih.gov/pubmed/37108549
http://dx.doi.org/10.3390/ijms24087389
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