Cargando…

Double Mutation of Days to Heading 2 and CONSTANS 3 Improves Agronomic Performance of Japonica Rice under Short Daylight Conditions in Southern China

Some progress has been made in understanding the pathways related to rice heading, but their applications to breeding japonica rice varieties adapted to grow in low-latitude areas (“indica to japonica”) are limited. We edited eight adaptation-related genes via a lab-established CRISPR/Cas9 system in...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Hongmei, Zhu, Yue, Wang, Linlin, Xiao, Chujian, Yuan, Jianming, Liu, Yao-Guang, Zhang, Qunyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138775/
https://www.ncbi.nlm.nih.gov/pubmed/37108508
http://dx.doi.org/10.3390/ijms24087346
_version_ 1785032786680217600
author Wang, Hongmei
Zhu, Yue
Wang, Linlin
Xiao, Chujian
Yuan, Jianming
Liu, Yao-Guang
Zhang, Qunyu
author_facet Wang, Hongmei
Zhu, Yue
Wang, Linlin
Xiao, Chujian
Yuan, Jianming
Liu, Yao-Guang
Zhang, Qunyu
author_sort Wang, Hongmei
collection PubMed
description Some progress has been made in understanding the pathways related to rice heading, but their applications to breeding japonica rice varieties adapted to grow in low-latitude areas (“indica to japonica”) are limited. We edited eight adaptation-related genes via a lab-established CRISPR/Cas9 system in a japonica variety, Shennong265 (SN265). All T(0) plants and their progeny bearing random mutation permutations were planted in southern China and screened for changes in heading date. We found that the double mutant of Days to heading 2 (DTH2) and CONSTANS 3 (OsCO3) (dth2-osco3), two CONSTANS-like (COL) genes, showed significantly delayed heading under both short-day (SD) and long-day (LD) conditions in Guangzhou and manifested great yield increase under SD conditions. We further demonstrated that the heading-related Hd3a-OsMADS14 pathway was down-regulated in the dth2-osco3 mutant lines. The editing of the COL genes DTH2 and OsCO3 greatly improves the agronomic performance of japonica rice in Southern China.
format Online
Article
Text
id pubmed-10138775
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101387752023-04-28 Double Mutation of Days to Heading 2 and CONSTANS 3 Improves Agronomic Performance of Japonica Rice under Short Daylight Conditions in Southern China Wang, Hongmei Zhu, Yue Wang, Linlin Xiao, Chujian Yuan, Jianming Liu, Yao-Guang Zhang, Qunyu Int J Mol Sci Communication Some progress has been made in understanding the pathways related to rice heading, but their applications to breeding japonica rice varieties adapted to grow in low-latitude areas (“indica to japonica”) are limited. We edited eight adaptation-related genes via a lab-established CRISPR/Cas9 system in a japonica variety, Shennong265 (SN265). All T(0) plants and their progeny bearing random mutation permutations were planted in southern China and screened for changes in heading date. We found that the double mutant of Days to heading 2 (DTH2) and CONSTANS 3 (OsCO3) (dth2-osco3), two CONSTANS-like (COL) genes, showed significantly delayed heading under both short-day (SD) and long-day (LD) conditions in Guangzhou and manifested great yield increase under SD conditions. We further demonstrated that the heading-related Hd3a-OsMADS14 pathway was down-regulated in the dth2-osco3 mutant lines. The editing of the COL genes DTH2 and OsCO3 greatly improves the agronomic performance of japonica rice in Southern China. MDPI 2023-04-16 /pmc/articles/PMC10138775/ /pubmed/37108508 http://dx.doi.org/10.3390/ijms24087346 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Wang, Hongmei
Zhu, Yue
Wang, Linlin
Xiao, Chujian
Yuan, Jianming
Liu, Yao-Guang
Zhang, Qunyu
Double Mutation of Days to Heading 2 and CONSTANS 3 Improves Agronomic Performance of Japonica Rice under Short Daylight Conditions in Southern China
title Double Mutation of Days to Heading 2 and CONSTANS 3 Improves Agronomic Performance of Japonica Rice under Short Daylight Conditions in Southern China
title_full Double Mutation of Days to Heading 2 and CONSTANS 3 Improves Agronomic Performance of Japonica Rice under Short Daylight Conditions in Southern China
title_fullStr Double Mutation of Days to Heading 2 and CONSTANS 3 Improves Agronomic Performance of Japonica Rice under Short Daylight Conditions in Southern China
title_full_unstemmed Double Mutation of Days to Heading 2 and CONSTANS 3 Improves Agronomic Performance of Japonica Rice under Short Daylight Conditions in Southern China
title_short Double Mutation of Days to Heading 2 and CONSTANS 3 Improves Agronomic Performance of Japonica Rice under Short Daylight Conditions in Southern China
title_sort double mutation of days to heading 2 and constans 3 improves agronomic performance of japonica rice under short daylight conditions in southern china
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138775/
https://www.ncbi.nlm.nih.gov/pubmed/37108508
http://dx.doi.org/10.3390/ijms24087346
work_keys_str_mv AT wanghongmei doublemutationofdaystoheading2andconstans3improvesagronomicperformanceofjaponicariceundershortdaylightconditionsinsouthernchina
AT zhuyue doublemutationofdaystoheading2andconstans3improvesagronomicperformanceofjaponicariceundershortdaylightconditionsinsouthernchina
AT wanglinlin doublemutationofdaystoheading2andconstans3improvesagronomicperformanceofjaponicariceundershortdaylightconditionsinsouthernchina
AT xiaochujian doublemutationofdaystoheading2andconstans3improvesagronomicperformanceofjaponicariceundershortdaylightconditionsinsouthernchina
AT yuanjianming doublemutationofdaystoheading2andconstans3improvesagronomicperformanceofjaponicariceundershortdaylightconditionsinsouthernchina
AT liuyaoguang doublemutationofdaystoheading2andconstans3improvesagronomicperformanceofjaponicariceundershortdaylightconditionsinsouthernchina
AT zhangqunyu doublemutationofdaystoheading2andconstans3improvesagronomicperformanceofjaponicariceundershortdaylightconditionsinsouthernchina