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Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study
Lung cancer is one of the most commonly diagnosed cancer types. Studying the molecular changes that occur in lung cancer is important to understand tumor formation and identify new therapeutic targets and early markers of the disease to decrease mortality. Glycosaminoglycan chains play important rol...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138872/ https://www.ncbi.nlm.nih.gov/pubmed/37108213 http://dx.doi.org/10.3390/ijms24087050 |
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author | Pál, Domonkos Tóth, Gábor Sugár, Simon Fügedi, Kata Dorina Szabó, Dániel Kovalszky, Ilona Papp, Dávid Schlosser, Gitta Tóth, Csaba Tornóczky, Tamás Drahos, László Turiák, Lilla |
author_facet | Pál, Domonkos Tóth, Gábor Sugár, Simon Fügedi, Kata Dorina Szabó, Dániel Kovalszky, Ilona Papp, Dávid Schlosser, Gitta Tóth, Csaba Tornóczky, Tamás Drahos, László Turiák, Lilla |
author_sort | Pál, Domonkos |
collection | PubMed |
description | Lung cancer is one of the most commonly diagnosed cancer types. Studying the molecular changes that occur in lung cancer is important to understand tumor formation and identify new therapeutic targets and early markers of the disease to decrease mortality. Glycosaminoglycan chains play important roles in various signaling events in the tumor microenvironment. Therefore, we have determined the quantity and sulfation characteristics of chondroitin sulfate and heparan sulfate in formalin-fixed paraffin-embedded human lung tissue samples belonging to different lung cancer types as well as tumor adjacent normal areas. Glycosaminoglycan disaccharide analysis was performed using HPLC-MS following on-surface lyase digestion. Significant changes were identified predominantly in the case of chondroitin sulfate; for example, the total amount was higher in tumor tissue compared to the adjacent normal tissue. We also observed differences in the degree of sulfation and relative proportions of individual chondroitin sulfate disaccharides between lung cancer types and adjacent normal tissue. Furthermore, the differences in the 6-O-/4-O-sulfation ratio of chondroitin sulfate were different between the lung cancer types. Our pilot study revealed that further investigation of the role of chondroitin sulfate chains and enzymes involved in their biosynthesis is an important aspect of lung cancer research. |
format | Online Article Text |
id | pubmed-10138872 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101388722023-04-28 Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study Pál, Domonkos Tóth, Gábor Sugár, Simon Fügedi, Kata Dorina Szabó, Dániel Kovalszky, Ilona Papp, Dávid Schlosser, Gitta Tóth, Csaba Tornóczky, Tamás Drahos, László Turiák, Lilla Int J Mol Sci Article Lung cancer is one of the most commonly diagnosed cancer types. Studying the molecular changes that occur in lung cancer is important to understand tumor formation and identify new therapeutic targets and early markers of the disease to decrease mortality. Glycosaminoglycan chains play important roles in various signaling events in the tumor microenvironment. Therefore, we have determined the quantity and sulfation characteristics of chondroitin sulfate and heparan sulfate in formalin-fixed paraffin-embedded human lung tissue samples belonging to different lung cancer types as well as tumor adjacent normal areas. Glycosaminoglycan disaccharide analysis was performed using HPLC-MS following on-surface lyase digestion. Significant changes were identified predominantly in the case of chondroitin sulfate; for example, the total amount was higher in tumor tissue compared to the adjacent normal tissue. We also observed differences in the degree of sulfation and relative proportions of individual chondroitin sulfate disaccharides between lung cancer types and adjacent normal tissue. Furthermore, the differences in the 6-O-/4-O-sulfation ratio of chondroitin sulfate were different between the lung cancer types. Our pilot study revealed that further investigation of the role of chondroitin sulfate chains and enzymes involved in their biosynthesis is an important aspect of lung cancer research. MDPI 2023-04-11 /pmc/articles/PMC10138872/ /pubmed/37108213 http://dx.doi.org/10.3390/ijms24087050 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pál, Domonkos Tóth, Gábor Sugár, Simon Fügedi, Kata Dorina Szabó, Dániel Kovalszky, Ilona Papp, Dávid Schlosser, Gitta Tóth, Csaba Tornóczky, Tamás Drahos, László Turiák, Lilla Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study |
title | Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study |
title_full | Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study |
title_fullStr | Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study |
title_full_unstemmed | Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study |
title_short | Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study |
title_sort | compositional analysis of glycosaminoglycans in different lung cancer types—a pilot study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138872/ https://www.ncbi.nlm.nih.gov/pubmed/37108213 http://dx.doi.org/10.3390/ijms24087050 |
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