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Morphological and Physiological Mechanisms of Melatonin on Delaying Drought-Induced Leaf Senescence in Cotton

Leaf senescence reduces the photosynthetic capacity of leaves, thus significantly affecting the growth, development, and yield formation of cotton. Melatonin (MT) is a multipotent substance proven to delay leaf senescence. However, its potential mechanism in delaying leaf senescence induced by abiot...

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Autores principales: Yang, Kai, Sun, Hongchun, Liu, Mengxing, Zhu, Lingxiao, Zhang, Ke, Zhang, Yongjiang, Li, Anchang, Zhang, Haina, Zhu, Jijie, Liu, Xiaoqing, Bai, Zhiying, Liu, Liantao, Li, Cundong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138977/
https://www.ncbi.nlm.nih.gov/pubmed/37108431
http://dx.doi.org/10.3390/ijms24087269
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author Yang, Kai
Sun, Hongchun
Liu, Mengxing
Zhu, Lingxiao
Zhang, Ke
Zhang, Yongjiang
Li, Anchang
Zhang, Haina
Zhu, Jijie
Liu, Xiaoqing
Bai, Zhiying
Liu, Liantao
Li, Cundong
author_facet Yang, Kai
Sun, Hongchun
Liu, Mengxing
Zhu, Lingxiao
Zhang, Ke
Zhang, Yongjiang
Li, Anchang
Zhang, Haina
Zhu, Jijie
Liu, Xiaoqing
Bai, Zhiying
Liu, Liantao
Li, Cundong
author_sort Yang, Kai
collection PubMed
description Leaf senescence reduces the photosynthetic capacity of leaves, thus significantly affecting the growth, development, and yield formation of cotton. Melatonin (MT) is a multipotent substance proven to delay leaf senescence. However, its potential mechanism in delaying leaf senescence induced by abiotic stress remains unclear. This study aimed to explore the effect of MT on delaying drought-induced leaf senescence in cotton seedlings and to clarify its morphological and physiological mechanisms. Drought stress upregulated the leaf senescence marker genes, destroyed the photosystem, and led to excessive accumulation of reactive oxygen species (ROS, e.g., H(2)O(2) and O(2)(−)), thus accelerating leaf senescence. However, leaf senescence was significantly delayed when 100 μM MT was sprayed on the leaves of the cotton seedlings. The delay was embodied by the increased chlorophyll content, photosynthetic capacity, and antioxidant enzyme activities, as well as decreased H(2)O(2), O(2)(−), and abscisic acid (ABA) contents by 34.44%, 37.68%, and 29.32%, respectively. MT significantly down-regulated chlorophyll degradation-related genes and senescence marker genes (GhNAC12 and GhWRKY27/71). In addition, MT reduced the chloroplast damage caused by drought-induced leaf senescence and maintained the integrity of the chloroplast lamellae structure under drought stress. The findings of this study collectively suggest that MT can effectively enhance the antioxidant enzyme system, improve photosynthetic efficiency, reduce chlorophyll degradation and ROS accumulation, and inhibit ABA synthesis, thereby delaying drought-induced leaf senescence in cotton.
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spelling pubmed-101389772023-04-28 Morphological and Physiological Mechanisms of Melatonin on Delaying Drought-Induced Leaf Senescence in Cotton Yang, Kai Sun, Hongchun Liu, Mengxing Zhu, Lingxiao Zhang, Ke Zhang, Yongjiang Li, Anchang Zhang, Haina Zhu, Jijie Liu, Xiaoqing Bai, Zhiying Liu, Liantao Li, Cundong Int J Mol Sci Article Leaf senescence reduces the photosynthetic capacity of leaves, thus significantly affecting the growth, development, and yield formation of cotton. Melatonin (MT) is a multipotent substance proven to delay leaf senescence. However, its potential mechanism in delaying leaf senescence induced by abiotic stress remains unclear. This study aimed to explore the effect of MT on delaying drought-induced leaf senescence in cotton seedlings and to clarify its morphological and physiological mechanisms. Drought stress upregulated the leaf senescence marker genes, destroyed the photosystem, and led to excessive accumulation of reactive oxygen species (ROS, e.g., H(2)O(2) and O(2)(−)), thus accelerating leaf senescence. However, leaf senescence was significantly delayed when 100 μM MT was sprayed on the leaves of the cotton seedlings. The delay was embodied by the increased chlorophyll content, photosynthetic capacity, and antioxidant enzyme activities, as well as decreased H(2)O(2), O(2)(−), and abscisic acid (ABA) contents by 34.44%, 37.68%, and 29.32%, respectively. MT significantly down-regulated chlorophyll degradation-related genes and senescence marker genes (GhNAC12 and GhWRKY27/71). In addition, MT reduced the chloroplast damage caused by drought-induced leaf senescence and maintained the integrity of the chloroplast lamellae structure under drought stress. The findings of this study collectively suggest that MT can effectively enhance the antioxidant enzyme system, improve photosynthetic efficiency, reduce chlorophyll degradation and ROS accumulation, and inhibit ABA synthesis, thereby delaying drought-induced leaf senescence in cotton. MDPI 2023-04-14 /pmc/articles/PMC10138977/ /pubmed/37108431 http://dx.doi.org/10.3390/ijms24087269 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Kai
Sun, Hongchun
Liu, Mengxing
Zhu, Lingxiao
Zhang, Ke
Zhang, Yongjiang
Li, Anchang
Zhang, Haina
Zhu, Jijie
Liu, Xiaoqing
Bai, Zhiying
Liu, Liantao
Li, Cundong
Morphological and Physiological Mechanisms of Melatonin on Delaying Drought-Induced Leaf Senescence in Cotton
title Morphological and Physiological Mechanisms of Melatonin on Delaying Drought-Induced Leaf Senescence in Cotton
title_full Morphological and Physiological Mechanisms of Melatonin on Delaying Drought-Induced Leaf Senescence in Cotton
title_fullStr Morphological and Physiological Mechanisms of Melatonin on Delaying Drought-Induced Leaf Senescence in Cotton
title_full_unstemmed Morphological and Physiological Mechanisms of Melatonin on Delaying Drought-Induced Leaf Senescence in Cotton
title_short Morphological and Physiological Mechanisms of Melatonin on Delaying Drought-Induced Leaf Senescence in Cotton
title_sort morphological and physiological mechanisms of melatonin on delaying drought-induced leaf senescence in cotton
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138977/
https://www.ncbi.nlm.nih.gov/pubmed/37108431
http://dx.doi.org/10.3390/ijms24087269
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