Cargando…

Juglone in Combination with Temozolomide Shows a Promising Epigenetic Therapeutic Effect on the Glioblastoma Cell Line

Glioblastoma (GBM) is the most common and aggressive primary brain tumor and one of the human malignancies with the highest mortality. Standard approaches for GBM, including gross total resection, radiotherapy, and chemotherapy, cannot destroy all the cancer cells, and despite advances in its treatm...

Descripción completa

Detalles Bibliográficos
Autores principales: Barciszewska, Anna-Maria, Belter, Agnieszka, Gawrońska, Iwona, Giel-Pietraszuk, Małgorzata, Naskręt-Barciszewska, Mirosława Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138991/
https://www.ncbi.nlm.nih.gov/pubmed/37108161
http://dx.doi.org/10.3390/ijms24086998
_version_ 1785032838363480064
author Barciszewska, Anna-Maria
Belter, Agnieszka
Gawrońska, Iwona
Giel-Pietraszuk, Małgorzata
Naskręt-Barciszewska, Mirosława Z.
author_facet Barciszewska, Anna-Maria
Belter, Agnieszka
Gawrońska, Iwona
Giel-Pietraszuk, Małgorzata
Naskręt-Barciszewska, Mirosława Z.
author_sort Barciszewska, Anna-Maria
collection PubMed
description Glioblastoma (GBM) is the most common and aggressive primary brain tumor and one of the human malignancies with the highest mortality. Standard approaches for GBM, including gross total resection, radiotherapy, and chemotherapy, cannot destroy all the cancer cells, and despite advances in its treatment, the prognosis for GBM remains poor. The problem is that we still do not understand what triggers GBM. Until now, the most successful chemotherapy with temozolomide for brain gliomas is not effective, and therefore new therapeutic strategies for GBM are needed. We found that juglone (J), which exhibits cytotoxic, anti-proliferative, and anti-invasive effects on various cells, could be a promising agent for GBM therapy. In this paper, we present the effects of juglone alone and in combination with temozolomide on glioblastoma cells. In addition to the analysis of cell viability and the cell cycle, we looked at the epigenetics effects of these compounds on cancer cells. We showed that juglone induces strong oxidative stress, as identified by a high increase in the amount of 8-oxo-dG, and decreases m(5)C in the DNA of cancer cells. In combination with TMZ, juglone modulates the level of both marker compounds. Our results strongly suggest that a combination of juglone and temozolomide can be applied for better GBM treatment.
format Online
Article
Text
id pubmed-10138991
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101389912023-04-28 Juglone in Combination with Temozolomide Shows a Promising Epigenetic Therapeutic Effect on the Glioblastoma Cell Line Barciszewska, Anna-Maria Belter, Agnieszka Gawrońska, Iwona Giel-Pietraszuk, Małgorzata Naskręt-Barciszewska, Mirosława Z. Int J Mol Sci Article Glioblastoma (GBM) is the most common and aggressive primary brain tumor and one of the human malignancies with the highest mortality. Standard approaches for GBM, including gross total resection, radiotherapy, and chemotherapy, cannot destroy all the cancer cells, and despite advances in its treatment, the prognosis for GBM remains poor. The problem is that we still do not understand what triggers GBM. Until now, the most successful chemotherapy with temozolomide for brain gliomas is not effective, and therefore new therapeutic strategies for GBM are needed. We found that juglone (J), which exhibits cytotoxic, anti-proliferative, and anti-invasive effects on various cells, could be a promising agent for GBM therapy. In this paper, we present the effects of juglone alone and in combination with temozolomide on glioblastoma cells. In addition to the analysis of cell viability and the cell cycle, we looked at the epigenetics effects of these compounds on cancer cells. We showed that juglone induces strong oxidative stress, as identified by a high increase in the amount of 8-oxo-dG, and decreases m(5)C in the DNA of cancer cells. In combination with TMZ, juglone modulates the level of both marker compounds. Our results strongly suggest that a combination of juglone and temozolomide can be applied for better GBM treatment. MDPI 2023-04-10 /pmc/articles/PMC10138991/ /pubmed/37108161 http://dx.doi.org/10.3390/ijms24086998 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barciszewska, Anna-Maria
Belter, Agnieszka
Gawrońska, Iwona
Giel-Pietraszuk, Małgorzata
Naskręt-Barciszewska, Mirosława Z.
Juglone in Combination with Temozolomide Shows a Promising Epigenetic Therapeutic Effect on the Glioblastoma Cell Line
title Juglone in Combination with Temozolomide Shows a Promising Epigenetic Therapeutic Effect on the Glioblastoma Cell Line
title_full Juglone in Combination with Temozolomide Shows a Promising Epigenetic Therapeutic Effect on the Glioblastoma Cell Line
title_fullStr Juglone in Combination with Temozolomide Shows a Promising Epigenetic Therapeutic Effect on the Glioblastoma Cell Line
title_full_unstemmed Juglone in Combination with Temozolomide Shows a Promising Epigenetic Therapeutic Effect on the Glioblastoma Cell Line
title_short Juglone in Combination with Temozolomide Shows a Promising Epigenetic Therapeutic Effect on the Glioblastoma Cell Line
title_sort juglone in combination with temozolomide shows a promising epigenetic therapeutic effect on the glioblastoma cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138991/
https://www.ncbi.nlm.nih.gov/pubmed/37108161
http://dx.doi.org/10.3390/ijms24086998
work_keys_str_mv AT barciszewskaannamaria jugloneincombinationwithtemozolomideshowsapromisingepigenetictherapeuticeffectontheglioblastomacellline
AT belteragnieszka jugloneincombinationwithtemozolomideshowsapromisingepigenetictherapeuticeffectontheglioblastomacellline
AT gawronskaiwona jugloneincombinationwithtemozolomideshowsapromisingepigenetictherapeuticeffectontheglioblastomacellline
AT gielpietraszukmałgorzata jugloneincombinationwithtemozolomideshowsapromisingepigenetictherapeuticeffectontheglioblastomacellline
AT naskretbarciszewskamirosławaz jugloneincombinationwithtemozolomideshowsapromisingepigenetictherapeuticeffectontheglioblastomacellline