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Assessing the Orthogonality of Phage-Encoded RNA Polymerases for Tailored Synthetic Biology Applications in Pseudomonas Species

The phage T7 RNA polymerase (RNAP) and lysozyme form the basis of the widely used pET expression system for recombinant expression in the biotechnology field and as a tool in microbial synthetic biology. Attempts to transfer this genetic circuitry from Escherichia coli to non-model bacterial organis...

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Detalles Bibliográficos
Autores principales:	Lammens, Eveline-Marie, Feyaerts, Nathalie, Kerremans, Alison, Boon, Maarten, Lavigne, Rob
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138996/ https://www.ncbi.nlm.nih.gov/pubmed/37108338 http://dx.doi.org/10.3390/ijms24087175

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