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Preliminary Study on the Effect of a Single High-Energy Electromagnetic Pulse on Morphology and Free Radical Generation in Human Mesenchymal Stem Cells

The effect of nanosecond electromagnetic pulses on human health, and especially on forming free radicals in human cells, is the subject of continuous research and ongoing discussion. This work presents a preliminary study on the effect of a single high-energy electromagnetic pulse on morphology, via...

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Autores principales: Czwartos, Joanna, Dobosz, Bernadeta, Kasprzycka, Wiktoria, Osuchowska, Paulina Natalia, Stępińska, Małgorzata, Trafny, Elżbieta Anna, Starzyński, Jacek, Mierczyk, Zygmunt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10139018/
https://www.ncbi.nlm.nih.gov/pubmed/37108409
http://dx.doi.org/10.3390/ijms24087246
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author Czwartos, Joanna
Dobosz, Bernadeta
Kasprzycka, Wiktoria
Osuchowska, Paulina Natalia
Stępińska, Małgorzata
Trafny, Elżbieta Anna
Starzyński, Jacek
Mierczyk, Zygmunt
author_facet Czwartos, Joanna
Dobosz, Bernadeta
Kasprzycka, Wiktoria
Osuchowska, Paulina Natalia
Stępińska, Małgorzata
Trafny, Elżbieta Anna
Starzyński, Jacek
Mierczyk, Zygmunt
author_sort Czwartos, Joanna
collection PubMed
description The effect of nanosecond electromagnetic pulses on human health, and especially on forming free radicals in human cells, is the subject of continuous research and ongoing discussion. This work presents a preliminary study on the effect of a single high-energy electromagnetic pulse on morphology, viability, and free radical generation in human mesenchymal stem cells (hMSC). The cells were exposed to a single electromagnetic pulse with an electric field magnitude of ~1 MV/m and a pulse duration of ~120 ns generated from a 600 kV Marx generator. The cell viability and morphology at 2 h and 24 h after exposure were examined using confocal fluorescent microscopy and scanning electron microscopy (SEM), respectively. The number of free radicals was investigated with electron paramagnetic resonance (EPR). The microscopic observations and EPR measurements showed that the exposure to the high-energy electromagnetic pulse influenced neither the number of free radicals generated nor the morphology of hMSC in vitro compared to control samples.
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spelling pubmed-101390182023-04-28 Preliminary Study on the Effect of a Single High-Energy Electromagnetic Pulse on Morphology and Free Radical Generation in Human Mesenchymal Stem Cells Czwartos, Joanna Dobosz, Bernadeta Kasprzycka, Wiktoria Osuchowska, Paulina Natalia Stępińska, Małgorzata Trafny, Elżbieta Anna Starzyński, Jacek Mierczyk, Zygmunt Int J Mol Sci Article The effect of nanosecond electromagnetic pulses on human health, and especially on forming free radicals in human cells, is the subject of continuous research and ongoing discussion. This work presents a preliminary study on the effect of a single high-energy electromagnetic pulse on morphology, viability, and free radical generation in human mesenchymal stem cells (hMSC). The cells were exposed to a single electromagnetic pulse with an electric field magnitude of ~1 MV/m and a pulse duration of ~120 ns generated from a 600 kV Marx generator. The cell viability and morphology at 2 h and 24 h after exposure were examined using confocal fluorescent microscopy and scanning electron microscopy (SEM), respectively. The number of free radicals was investigated with electron paramagnetic resonance (EPR). The microscopic observations and EPR measurements showed that the exposure to the high-energy electromagnetic pulse influenced neither the number of free radicals generated nor the morphology of hMSC in vitro compared to control samples. MDPI 2023-04-14 /pmc/articles/PMC10139018/ /pubmed/37108409 http://dx.doi.org/10.3390/ijms24087246 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Czwartos, Joanna
Dobosz, Bernadeta
Kasprzycka, Wiktoria
Osuchowska, Paulina Natalia
Stępińska, Małgorzata
Trafny, Elżbieta Anna
Starzyński, Jacek
Mierczyk, Zygmunt
Preliminary Study on the Effect of a Single High-Energy Electromagnetic Pulse on Morphology and Free Radical Generation in Human Mesenchymal Stem Cells
title Preliminary Study on the Effect of a Single High-Energy Electromagnetic Pulse on Morphology and Free Radical Generation in Human Mesenchymal Stem Cells
title_full Preliminary Study on the Effect of a Single High-Energy Electromagnetic Pulse on Morphology and Free Radical Generation in Human Mesenchymal Stem Cells
title_fullStr Preliminary Study on the Effect of a Single High-Energy Electromagnetic Pulse on Morphology and Free Radical Generation in Human Mesenchymal Stem Cells
title_full_unstemmed Preliminary Study on the Effect of a Single High-Energy Electromagnetic Pulse on Morphology and Free Radical Generation in Human Mesenchymal Stem Cells
title_short Preliminary Study on the Effect of a Single High-Energy Electromagnetic Pulse on Morphology and Free Radical Generation in Human Mesenchymal Stem Cells
title_sort preliminary study on the effect of a single high-energy electromagnetic pulse on morphology and free radical generation in human mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10139018/
https://www.ncbi.nlm.nih.gov/pubmed/37108409
http://dx.doi.org/10.3390/ijms24087246
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