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Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium

BACKGROUND: As part of a study aimed at developing new pharmaceutical products from natural resources, the purpose of this research was twofold: (1) to fractionate crude extracts from the bark of Mahonia aquifolium and (2) to evaluate the strength of the antimutagenic activity of the separate compon...

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Autores principales: Čerňáková, Marta, Košt'álová, Daniela, Kettmann, Viktor, Plodová, Miriam, Tóth, Jaroslav, Dřímal, Ján
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC101396/
https://www.ncbi.nlm.nih.gov/pubmed/11943071
http://dx.doi.org/10.1186/1472-6882-2-2
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author Čerňáková, Marta
Košt'álová, Daniela
Kettmann, Viktor
Plodová, Miriam
Tóth, Jaroslav
Dřímal, Ján
author_facet Čerňáková, Marta
Košt'álová, Daniela
Kettmann, Viktor
Plodová, Miriam
Tóth, Jaroslav
Dřímal, Ján
author_sort Čerňáková, Marta
collection PubMed
description BACKGROUND: As part of a study aimed at developing new pharmaceutical products from natural resources, the purpose of this research was twofold: (1) to fractionate crude extracts from the bark of Mahonia aquifolium and (2) to evaluate the strength of the antimutagenic activity of the separate components against one of the common direct-acting chemical mutagens. METHODS: The antimutagenic potency was evaluated against acridine orange (AO) by using Euglena gracilis as an eukaryotic test model, based on the ability of the test compound/fraction to prevent the mutagen-induced damage of chloroplast DNA. RESULTS: It was found that the antimutagenicity of the crude Mahonia extract resides in both bis-benzylisoquinoline (BBI) and protoberberine alkaloid fractions but only the protoberberine derivatives, jatrorrhizine and berberine, showed significant concentration-dependent inhibitory effect against the AO-induced chloroplast mutagenesis of E. gracilis. Especially berberine elicited, at a very low dose, remarkable suppression of the AO-induced mutagenicity, its antimutagenic potency being almost three orders of magnitude higher when compared to its close analogue, jatrorrhizine. Possible mechanisms of the antimutagenic action are discussed in terms of recent literature data. While the potent antimutagenic activity of the protoberberines most likely results from the inhibition of DNA topoisomerase I, the actual mechanism(s) for the BBI alkaloids is hard to be identified. CONCLUSIONS: Taken together, the results indicate that berberine possesses promising antimutagenic/anticarcinogenic potential that is worth to be investigated further.
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spelling pubmed-1013962002-04-11 Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium Čerňáková, Marta Košt'álová, Daniela Kettmann, Viktor Plodová, Miriam Tóth, Jaroslav Dřímal, Ján BMC Complement Altern Med Research Article BACKGROUND: As part of a study aimed at developing new pharmaceutical products from natural resources, the purpose of this research was twofold: (1) to fractionate crude extracts from the bark of Mahonia aquifolium and (2) to evaluate the strength of the antimutagenic activity of the separate components against one of the common direct-acting chemical mutagens. METHODS: The antimutagenic potency was evaluated against acridine orange (AO) by using Euglena gracilis as an eukaryotic test model, based on the ability of the test compound/fraction to prevent the mutagen-induced damage of chloroplast DNA. RESULTS: It was found that the antimutagenicity of the crude Mahonia extract resides in both bis-benzylisoquinoline (BBI) and protoberberine alkaloid fractions but only the protoberberine derivatives, jatrorrhizine and berberine, showed significant concentration-dependent inhibitory effect against the AO-induced chloroplast mutagenesis of E. gracilis. Especially berberine elicited, at a very low dose, remarkable suppression of the AO-induced mutagenicity, its antimutagenic potency being almost three orders of magnitude higher when compared to its close analogue, jatrorrhizine. Possible mechanisms of the antimutagenic action are discussed in terms of recent literature data. While the potent antimutagenic activity of the protoberberines most likely results from the inhibition of DNA topoisomerase I, the actual mechanism(s) for the BBI alkaloids is hard to be identified. CONCLUSIONS: Taken together, the results indicate that berberine possesses promising antimutagenic/anticarcinogenic potential that is worth to be investigated further. BioMed Central 2002-02-19 /pmc/articles/PMC101396/ /pubmed/11943071 http://dx.doi.org/10.1186/1472-6882-2-2 Text en Copyright © 2002 Čerňáková et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Čerňáková, Marta
Košt'álová, Daniela
Kettmann, Viktor
Plodová, Miriam
Tóth, Jaroslav
Dřímal, Ján
Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium
title Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium
title_full Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium
title_fullStr Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium
title_full_unstemmed Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium
title_short Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium
title_sort potential antimutagenic activity of berberine, a constituent of mahonia aquifolium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC101396/
https://www.ncbi.nlm.nih.gov/pubmed/11943071
http://dx.doi.org/10.1186/1472-6882-2-2
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