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Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson’s Disease
Mitochondrial ferritin (FtMt) is an endogenous iron-storage protein localized in the mitochondria. FtMt is mainly observed in restricted tissues, such as those in the testis, islets of Langerhans, and brain. Further, it may protect cells from oxidative stress in neurodegenerative diseases, including...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10139838/ https://www.ncbi.nlm.nih.gov/pubmed/37124956 http://dx.doi.org/10.1267/ahc.22-00109 |
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author | Tsubaki, Haruka Yanagisawa, Daijiro Kageyama, Yusuke Hafiz Abu Baker, Zulzikry Mukaisho, Ken-ichi Tooyama, Ikuo |
author_facet | Tsubaki, Haruka Yanagisawa, Daijiro Kageyama, Yusuke Hafiz Abu Baker, Zulzikry Mukaisho, Ken-ichi Tooyama, Ikuo |
author_sort | Tsubaki, Haruka |
collection | PubMed |
description | Mitochondrial ferritin (FtMt) is an endogenous iron-storage protein localized in the mitochondria. FtMt is mainly observed in restricted tissues, such as those in the testis, islets of Langerhans, and brain. Further, it may protect cells from oxidative stress in neurodegenerative diseases, including Alzheimer’s disease and progressive supranuclear palsy. However, the role of FtMt in Parkinson’s disease (PD) remains unclear. Therefore, the current study investigated the localization and expression level of FtMt in the midbrain of patients with PD and healthy controls using immunohistochemical techniques. FtMt immunoreactivity was mainly detected in dopaminergic neurons in the substantia nigra pars compacta (SNc) in both healthy controls and patients with PD. In addition, FtMt-positive particles were observed outside the dopaminergic neurons in patients with PD. Based on a quantitative comparison, patients with PD had a significantly upregulated FtMt immunoreactivity in dopaminergic neurons than healthy controls. Our result might be helpful in future studies on the role of FtMt in PD. |
format | Online Article Text |
id | pubmed-10139838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY |
record_format | MEDLINE/PubMed |
spelling | pubmed-101398382023-04-28 Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson’s Disease Tsubaki, Haruka Yanagisawa, Daijiro Kageyama, Yusuke Hafiz Abu Baker, Zulzikry Mukaisho, Ken-ichi Tooyama, Ikuo Acta Histochem Cytochem Regular Article Mitochondrial ferritin (FtMt) is an endogenous iron-storage protein localized in the mitochondria. FtMt is mainly observed in restricted tissues, such as those in the testis, islets of Langerhans, and brain. Further, it may protect cells from oxidative stress in neurodegenerative diseases, including Alzheimer’s disease and progressive supranuclear palsy. However, the role of FtMt in Parkinson’s disease (PD) remains unclear. Therefore, the current study investigated the localization and expression level of FtMt in the midbrain of patients with PD and healthy controls using immunohistochemical techniques. FtMt immunoreactivity was mainly detected in dopaminergic neurons in the substantia nigra pars compacta (SNc) in both healthy controls and patients with PD. In addition, FtMt-positive particles were observed outside the dopaminergic neurons in patients with PD. Based on a quantitative comparison, patients with PD had a significantly upregulated FtMt immunoreactivity in dopaminergic neurons than healthy controls. Our result might be helpful in future studies on the role of FtMt in PD. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2023-04-25 2023-04-08 /pmc/articles/PMC10139838/ /pubmed/37124956 http://dx.doi.org/10.1267/ahc.22-00109 Text en 2023 The Japan Society of Histochemistry and Cytochemistry https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC-BY-NC), which permits use, distribution and reproduction of the articles in any medium provided that the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Regular Article Tsubaki, Haruka Yanagisawa, Daijiro Kageyama, Yusuke Hafiz Abu Baker, Zulzikry Mukaisho, Ken-ichi Tooyama, Ikuo Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson’s Disease |
title | Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson’s Disease |
title_full | Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson’s Disease |
title_fullStr | Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson’s Disease |
title_full_unstemmed | Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson’s Disease |
title_short | Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson’s Disease |
title_sort | immunohistochemical analysis of mitochondrial ferritin in the midbrain of patients with parkinson’s disease |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10139838/ https://www.ncbi.nlm.nih.gov/pubmed/37124956 http://dx.doi.org/10.1267/ahc.22-00109 |
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