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Marginal zone B cells are responsible for the production of alloantibodies following platelet transfusion in mice
Alloimmunization against platelets remains a potentially serious adverse transfusion event. Alloantibodies produced by the recipient, mainly directed against human leukocyte antigen class I donor antigens, can compromise the therapeutic efficacy of subsequent transfusions, and may lead to refractori...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society of Hematology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10139948/ https://www.ncbi.nlm.nih.gov/pubmed/36490266 http://dx.doi.org/10.1182/bloodadvances.2022008411 |
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author | Couvidou, Adèle Angénieux, Catherine Ruch, Laurie Mangin, Pierre H. Gachet, Christian Maître, Blandine |
author_facet | Couvidou, Adèle Angénieux, Catherine Ruch, Laurie Mangin, Pierre H. Gachet, Christian Maître, Blandine |
author_sort | Couvidou, Adèle |
collection | PubMed |
description | Alloimmunization against platelets remains a potentially serious adverse transfusion event. Alloantibodies produced by the recipient, mainly directed against human leukocyte antigen class I donor antigens, can compromise the therapeutic efficacy of subsequent transfusions, and may lead to refractoriness. Because the mechanism of anti-HLA alloantibody formation is poorly understood, this study aimed to identify the cells involved in the platelet immune response by focusing on the spleen, the main organ that orchestrates this alloimmune response. In the spleen, transfused allogeneic platelets are located in the marginal zone and interact with marginal zone B (MZB) cells, a specialized B-cell population implicated in the capture and follicular delivery of blood-borne antigens. To study the involvement of MZB cells in alloantibody production, we used a murine model reproducing major histocompatibility complex incompatibility between a donor (H2(b)) and recipient (H2(d)) that occurs during platelet transfusion. Following weekly H2(b) platelet transfusions, recipient H2(d) mice produced anti-H2(b) immunoglobulin G, which induced a refractory state upon subsequent transfusions. Specific immunodepletion of MZB cells or their displacement from the marginal zone to the B-cell follicles by treatment with an S1P1 antagonist before each transfusion prevented significant alloantibody formation. Under these conditions, transfused platelets were still circulating after 24 hours, whereas they were rapidly removed from circulation in alloimmunized mice. The identification of MZB cells as key players in the platelet alloimmune response opens up new perspectives for minimizing platelet alloimmunization and avoiding the associated refractory state in frequently transfused patients. |
format | Online Article Text |
id | pubmed-10139948 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The American Society of Hematology |
record_format | MEDLINE/PubMed |
spelling | pubmed-101399482023-04-29 Marginal zone B cells are responsible for the production of alloantibodies following platelet transfusion in mice Couvidou, Adèle Angénieux, Catherine Ruch, Laurie Mangin, Pierre H. Gachet, Christian Maître, Blandine Blood Adv Transfusion Medicine Alloimmunization against platelets remains a potentially serious adverse transfusion event. Alloantibodies produced by the recipient, mainly directed against human leukocyte antigen class I donor antigens, can compromise the therapeutic efficacy of subsequent transfusions, and may lead to refractoriness. Because the mechanism of anti-HLA alloantibody formation is poorly understood, this study aimed to identify the cells involved in the platelet immune response by focusing on the spleen, the main organ that orchestrates this alloimmune response. In the spleen, transfused allogeneic platelets are located in the marginal zone and interact with marginal zone B (MZB) cells, a specialized B-cell population implicated in the capture and follicular delivery of blood-borne antigens. To study the involvement of MZB cells in alloantibody production, we used a murine model reproducing major histocompatibility complex incompatibility between a donor (H2(b)) and recipient (H2(d)) that occurs during platelet transfusion. Following weekly H2(b) platelet transfusions, recipient H2(d) mice produced anti-H2(b) immunoglobulin G, which induced a refractory state upon subsequent transfusions. Specific immunodepletion of MZB cells or their displacement from the marginal zone to the B-cell follicles by treatment with an S1P1 antagonist before each transfusion prevented significant alloantibody formation. Under these conditions, transfused platelets were still circulating after 24 hours, whereas they were rapidly removed from circulation in alloimmunized mice. The identification of MZB cells as key players in the platelet alloimmune response opens up new perspectives for minimizing platelet alloimmunization and avoiding the associated refractory state in frequently transfused patients. The American Society of Hematology 2022-12-12 /pmc/articles/PMC10139948/ /pubmed/36490266 http://dx.doi.org/10.1182/bloodadvances.2022008411 Text en © 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Transfusion Medicine Couvidou, Adèle Angénieux, Catherine Ruch, Laurie Mangin, Pierre H. Gachet, Christian Maître, Blandine Marginal zone B cells are responsible for the production of alloantibodies following platelet transfusion in mice |
title | Marginal zone B cells are responsible for the production of alloantibodies following platelet transfusion in mice |
title_full | Marginal zone B cells are responsible for the production of alloantibodies following platelet transfusion in mice |
title_fullStr | Marginal zone B cells are responsible for the production of alloantibodies following platelet transfusion in mice |
title_full_unstemmed | Marginal zone B cells are responsible for the production of alloantibodies following platelet transfusion in mice |
title_short | Marginal zone B cells are responsible for the production of alloantibodies following platelet transfusion in mice |
title_sort | marginal zone b cells are responsible for the production of alloantibodies following platelet transfusion in mice |
topic | Transfusion Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10139948/ https://www.ncbi.nlm.nih.gov/pubmed/36490266 http://dx.doi.org/10.1182/bloodadvances.2022008411 |
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