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Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis

Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) allows the dissection of transcriptional changes in immune cells with HIV infection. Here, we isolate PBMCs from HIV-infected individuals. After counting the cell number and verifying cell viability, we perform scRN...

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Detalles Bibliográficos
Autores principales:	Jia, Jie, Zhao, Yu, Wang, Jian-Hua, Kuang, Yi-Qun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140154/ https://www.ncbi.nlm.nih.gov/pubmed/37060557 http://dx.doi.org/10.1016/j.xpro.2023.102222

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