Cargando…

A widely applicable and cost-effective method for specific RNA–protein complex isolation

Although methodological advances have been made over the past years, a widely applicable, easily scalable and cost-effective procedure that can be routinely used to isolate specific ribonucleoprotein complexes (RNPs) remains elusive. We describe the “Silica-based Acidic Phase Separation (SAPS)-captu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Balzarini, Sam, Van Ende, Roosje, Voet, Arnout, Geuten, Koen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140378/ https://www.ncbi.nlm.nih.gov/pubmed/37106019 http://dx.doi.org/10.1038/s41598-023-34157-0

Ejemplares similares

Single and Combined Methods to Specifically or Bulk-Purify RNA–Protein Complexes
por: Van Ende, Roosje, et al.
Publicado: (2020)

Development and applications of artificial symmetrical proteins
por: Vrancken, Jeroen P.M., et al.
Publicado: (2020)

Heterochronic genes in plant evolution and development
por: Geuten, Koen, et al.
Publicado: (2013)

The Role of FLOWERING LOCUS C Relatives in Cereals
por: Kennedy, Alice, et al.
Publicado: (2020)

Protein interaction evolution from promiscuity to specificity with reduced flexibility in an increasingly complex network
por: Alhindi, T., et al.
Publicado: (2017)

Electrostatic Similarities between Protein and Small Molecule Ligands Facilitate the Design of Protein-Protein Interaction Inhibitors
por: Voet, Arnout, et al.
Publicado: (2013)

UV crosslinked mRNA-binding proteins captured from leaf mesophyll protoplasts
por: Zhang, Zhicheng, et al.
Publicado: (2016)

FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution
por: Huang, Gang, et al.
Publicado: (2019)

TM8 represses developmental timing in Nicotiana benthamiana and has functionally diversified in angiosperms
por: Coenen, Heleen, et al.
Publicado: (2018)

An integrated map of HIV genome-wide variation from a population perspective
por: Li, Guangdi, et al.
Publicado: (2015)

Fragger: a protein fragment picker for structural queries
por: Berenger, Francois, et al.
Publicado: (2018)

Expression divergence of the AGL6 MADS domain transcription factor lineage after a core eudicot duplication suggests functional diversification
por: Viaene, Tom, et al.
Publicado: (2010)

The Effect of Ambient Temperature on Brachypodium distachyon Development
por: Li, Meixia, et al.
Publicado: (2019)

Cold Induced Antisense Transcription of FLOWERING LOCUS C in Distant Grasses
por: Jiao, Fuchao, et al.
Publicado: (2019)

In silico, in vitro and cellular analysis with a kinome-wide inhibitor panel correlates cellular LRRK2 dephosphorylation to inhibitor activity on LRRK2
por: Vancraenenbroeck, Renée, et al.
Publicado: (2014)

RF15 | PMON291 Crystal Structures of Androgen DNA Binding Domain Interacting with its Response Elements (C3(1) ARE and MMTV-GRE)
por: Claessens, Frank, et al.
Publicado: (2022)

Structure and stability of the designer protein WRAP-T and its permutants
por: Mylemans, Bram, et al.
Publicado: (2021)

Ribo-Pop: simple, cost-effective, and widely applicable ribosomal RNA depletion
por: Thompson, Mary Kay, et al.
Publicado: (2020)

A rotation-translation invariant molecular descriptor of partial charges and its use in ligand-based virtual screening
por: Berenger, Francois, et al.
Publicado: (2014)

Methods and applications for single-cell and spatial multi-omics
por: Vandereyken, Katy, et al.
Publicado: (2023)

Insights Into Natural Genetic Resistance to Rice Yellow Mottle Virus and Implications on Breeding for Durable Resistance
por: Odongo, Patrick J., et al.
Publicado: (2021)

Molecular assemblies built with the artificial protein Pizza
por: Vrancken, Jeroen P.M., et al.
Publicado: (2020)

Mapping the Binding Interface between an HIV-1 Inhibiting Intrabody and the Viral Protein Rev
por: Vercruysse, Thomas, et al.
Publicado: (2013)

Functional conservation of HIV-1 Gag: implications for rational drug design
por: Li, Guangdi, et al.
Publicado: (2013)

The T850D Phosphomimetic Mutation in the Androgen Receptor Ligand Binding Domain Enhances Recruitment at Activation Function 2
por: Helsen, Christine, et al.
Publicado: (2022)

QRREM method for the isolation of high-quality RNA from the complex matrices of coconut
por: Iqbal, Amjad, et al.
Publicado: (2019)

Discovery of a potent protein kinase D inhibitor: insights in the binding mode of pyrazolo[3,4-d]pyrimidine analogues
por: Verschueren, Klaas, et al.
Publicado: (2017)

Genome-wide analysis of the plant-specific PLATZ proteins in maize and identification of their general role in interaction with RNA polymerase III complex
por: Wang, Jiechen, et al.
Publicado: (2018)

Evolution of DNA-Binding Sites of a Floral Master Regulatory Transcription Factor
por: Muiño, Jose M., et al.
Publicado: (2016)

TU-tagging: cell type specific RNA isolation from intact complex tissues
por: Miller, Michael R., et al.
Publicado: (2009)

Meningococcal Serogroup A, C, W(135) and Y Conjugated Vaccine: A Cost-Effectiveness Analysis in the Netherlands
por: Hepkema, Hiltsje, et al.
Publicado: (2013)

High mannose-specific lectin Msl mediates key interactions of the vaginal Lactobacillus plantarum isolate CMPG5300
por: Malik, Shweta, et al.
Publicado: (2016)

Genome-Wide Identification of Alternatively Spliced mRNA Targets of Specific RNA-Binding Proteins
por: Robida, Mark D., et al.
Publicado: (2007)

Probing the applicability of autotransporter based surface display with the EstA autotransporter of Pseudomonas stutzeri A15
por: Nicolay, Toon, et al.
Publicado: (2012)

Reconstruction of Ancestral Metabolic Enzymes Reveals Molecular Mechanisms Underlying Evolutionary Innovation through Gene Duplication
por: Voordeckers, Karin, et al.
Publicado: (2012)

Application of the Activity-Based Costing Method for Unit-Cost Calculation in a Hospital
por: Javid, Mahdi, et al.
Publicado: (2016)

Isolation methods for particle protein corona complexes from protein-rich matrices
por: Böhmert, Linda, et al.
Publicado: (2020)

A robust, cost-effective and widely applicable whole-genome sequencing protocol for capripoxviruses
por: Mathijs, Elisabeth, et al.
Publicado: (2022)

Co-expression of RNA–protein complexes in Escherichia coli and applications to RNA biology
por: Ponchon, Luc, et al.
Publicado: (2013)

Isolation and genome-wide characterization of cellular DNA:RNA triplex structures
por: Sentürk Cetin, Nevcin, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_4eqgupoapfn8mff031im3ms0ba