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GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway

Clinically, COVID-19 is often accompanied by a severe immune response (cytokine storm) which produces a large number of cytokines, such as TNF-α, IL-6 and IL-12, and consequently causes acute respiratory distress syndrome (ARDS). GMI is a type of fungal immunomodulatory protein that is cloned from G...

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Autores principales: Lin, Zhi-Hu, Yeh, Hsin, Lo, Hung-Chih, Hua, Wei-Jyun, Ni, Ming-Yang, Wang, Li-Kai, Chang, Ting-Ting, Yang, Muh-Hwa, Lin, Tung-Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140468/
https://www.ncbi.nlm.nih.gov/pubmed/37119883
http://dx.doi.org/10.1016/j.ijbiomac.2023.124648
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author Lin, Zhi-Hu
Yeh, Hsin
Lo, Hung-Chih
Hua, Wei-Jyun
Ni, Ming-Yang
Wang, Li-Kai
Chang, Ting-Ting
Yang, Muh-Hwa
Lin, Tung-Yi
author_facet Lin, Zhi-Hu
Yeh, Hsin
Lo, Hung-Chih
Hua, Wei-Jyun
Ni, Ming-Yang
Wang, Li-Kai
Chang, Ting-Ting
Yang, Muh-Hwa
Lin, Tung-Yi
author_sort Lin, Zhi-Hu
collection PubMed
description Clinically, COVID-19 is often accompanied by a severe immune response (cytokine storm) which produces a large number of cytokines, such as TNF-α, IL-6 and IL-12, and consequently causes acute respiratory distress syndrome (ARDS). GMI is a type of fungal immunomodulatory protein that is cloned from Ganoderma microsporum and acts as modulating immunocyte for various inflammatory diseases. This study identifies GMI as a potential anti-inflammatory agent and determines the effects of GMI on the inhibition of SARS-CoV-2-induced cytokine secretion. Functional studies showed that SARS-CoV-2 envelope (E) protein induces inflammatory process in murine macrophages RAW264.7 and MH-S cells and in phorbol 12-myristate 13-acetate (PMA)-stimulated human THP-1 cells. GMI exhibits a strong inhibitory effect for SARS-CoV-2-E-induced pro-inflammatory mediators, including NO, TNF-α, IL-6, and IL-12 in macrophages. GMI reduces SARS-CoV-2-E-induced intracellular inflammatory molecules, such as iNOS and COX-2, and inhibits SARS-CoV-2-E-stimulated phosphorylation of ERK1/2 and P38. GMI also downregulates pro-inflammatory cytokine levels in lung tissue and serum after the mice inhale SARS-CoV-2-E protein. In conclusion, this study shows that GMI acts as an agent to alleviate SARS-CoV-2-E-induced inflammation.
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spelling pubmed-101404682023-04-28 GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway Lin, Zhi-Hu Yeh, Hsin Lo, Hung-Chih Hua, Wei-Jyun Ni, Ming-Yang Wang, Li-Kai Chang, Ting-Ting Yang, Muh-Hwa Lin, Tung-Yi Int J Biol Macromol Article Clinically, COVID-19 is often accompanied by a severe immune response (cytokine storm) which produces a large number of cytokines, such as TNF-α, IL-6 and IL-12, and consequently causes acute respiratory distress syndrome (ARDS). GMI is a type of fungal immunomodulatory protein that is cloned from Ganoderma microsporum and acts as modulating immunocyte for various inflammatory diseases. This study identifies GMI as a potential anti-inflammatory agent and determines the effects of GMI on the inhibition of SARS-CoV-2-induced cytokine secretion. Functional studies showed that SARS-CoV-2 envelope (E) protein induces inflammatory process in murine macrophages RAW264.7 and MH-S cells and in phorbol 12-myristate 13-acetate (PMA)-stimulated human THP-1 cells. GMI exhibits a strong inhibitory effect for SARS-CoV-2-E-induced pro-inflammatory mediators, including NO, TNF-α, IL-6, and IL-12 in macrophages. GMI reduces SARS-CoV-2-E-induced intracellular inflammatory molecules, such as iNOS and COX-2, and inhibits SARS-CoV-2-E-stimulated phosphorylation of ERK1/2 and P38. GMI also downregulates pro-inflammatory cytokine levels in lung tissue and serum after the mice inhale SARS-CoV-2-E protein. In conclusion, this study shows that GMI acts as an agent to alleviate SARS-CoV-2-E-induced inflammation. Elsevier B.V. 2023-06-30 2023-04-28 /pmc/articles/PMC10140468/ /pubmed/37119883 http://dx.doi.org/10.1016/j.ijbiomac.2023.124648 Text en © 2023 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Lin, Zhi-Hu
Yeh, Hsin
Lo, Hung-Chih
Hua, Wei-Jyun
Ni, Ming-Yang
Wang, Li-Kai
Chang, Ting-Ting
Yang, Muh-Hwa
Lin, Tung-Yi
GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway
title GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway
title_full GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway
title_fullStr GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway
title_full_unstemmed GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway
title_short GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway
title_sort gmi, a fungal immunomodulatory protein, ameliorates sars-cov-2 envelope protein-induced inflammation in macrophages via inhibition of mapk pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140468/
https://www.ncbi.nlm.nih.gov/pubmed/37119883
http://dx.doi.org/10.1016/j.ijbiomac.2023.124648
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