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Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways

The importance of the cladoceran Daphnia as a model organism for ecotoxicity testing has been well-established since the 1980s. Daphnia have been increasingly used in standardised testing of chemicals as they are well characterised and show sensitivity to pollutants, making them an essential indicat...

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Autores principales: Reilly, Katie, Ellis, Laura-Jayne A., Davoudi, Hossein Hayat, Supian, Suffeiya, Maia, Marcella T., Silva, Gabriela H., Guo, Zhiling, Martinez, Diego Stéfani T., Lynch, Iseult
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140508/
https://www.ncbi.nlm.nih.gov/pubmed/37124970
http://dx.doi.org/10.3389/ftox.2023.1178482
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author Reilly, Katie
Ellis, Laura-Jayne A.
Davoudi, Hossein Hayat
Supian, Suffeiya
Maia, Marcella T.
Silva, Gabriela H.
Guo, Zhiling
Martinez, Diego Stéfani T.
Lynch, Iseult
author_facet Reilly, Katie
Ellis, Laura-Jayne A.
Davoudi, Hossein Hayat
Supian, Suffeiya
Maia, Marcella T.
Silva, Gabriela H.
Guo, Zhiling
Martinez, Diego Stéfani T.
Lynch, Iseult
author_sort Reilly, Katie
collection PubMed
description The importance of the cladoceran Daphnia as a model organism for ecotoxicity testing has been well-established since the 1980s. Daphnia have been increasingly used in standardised testing of chemicals as they are well characterised and show sensitivity to pollutants, making them an essential indicator species for environmental stress. The mapping of the genomes of D. pulex in 2012 and D. magna in 2017 further consolidated their utility for ecotoxicity testing, including demonstrating the responsiveness of the Daphnia genome to environmental stressors. The short lifecycle and parthenogenetic reproduction make Daphnia useful for assessment of developmental toxicity and adaption to stress. The emergence of nanomaterials (NMs) and their safety assessment has introduced some challenges to the use of standard toxicity tests which were developed for soluble chemicals. NMs have enormous reactive surface areas resulting in dynamic interactions with dissolved organic carbon, proteins and other biomolecules in their surroundings leading to a myriad of physical, chemical, biological, and macromolecular transformations of the NMs and thus changes in their bioavailability to, and impacts on, daphnids. However, NM safety assessments are also driving innovations in our approaches to toxicity testing, for both chemicals and other emerging contaminants such as microplastics (MPs). These advances include establishing more realistic environmental exposures via medium composition tuning including pre-conditioning by the organisms to provide relevant biomolecules as background, development of microfluidics approaches to mimic environmental flow conditions typical in streams, utilisation of field daphnids cultured in the lab to assess adaption and impacts of pre-exposure to pollution gradients, and of course development of mechanistic insights to connect the first encounter with NMs or MPs to an adverse outcome, via the key events in an adverse outcome pathway. Insights into these developments are presented below to inspire further advances and utilisation of these important organisms as part of an overall environmental risk assessment of NMs and MPs impacts, including in mixture exposure scenarios.
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spelling pubmed-101405082023-04-29 Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways Reilly, Katie Ellis, Laura-Jayne A. Davoudi, Hossein Hayat Supian, Suffeiya Maia, Marcella T. Silva, Gabriela H. Guo, Zhiling Martinez, Diego Stéfani T. Lynch, Iseult Front Toxicol Toxicology The importance of the cladoceran Daphnia as a model organism for ecotoxicity testing has been well-established since the 1980s. Daphnia have been increasingly used in standardised testing of chemicals as they are well characterised and show sensitivity to pollutants, making them an essential indicator species for environmental stress. The mapping of the genomes of D. pulex in 2012 and D. magna in 2017 further consolidated their utility for ecotoxicity testing, including demonstrating the responsiveness of the Daphnia genome to environmental stressors. The short lifecycle and parthenogenetic reproduction make Daphnia useful for assessment of developmental toxicity and adaption to stress. The emergence of nanomaterials (NMs) and their safety assessment has introduced some challenges to the use of standard toxicity tests which were developed for soluble chemicals. NMs have enormous reactive surface areas resulting in dynamic interactions with dissolved organic carbon, proteins and other biomolecules in their surroundings leading to a myriad of physical, chemical, biological, and macromolecular transformations of the NMs and thus changes in their bioavailability to, and impacts on, daphnids. However, NM safety assessments are also driving innovations in our approaches to toxicity testing, for both chemicals and other emerging contaminants such as microplastics (MPs). These advances include establishing more realistic environmental exposures via medium composition tuning including pre-conditioning by the organisms to provide relevant biomolecules as background, development of microfluidics approaches to mimic environmental flow conditions typical in streams, utilisation of field daphnids cultured in the lab to assess adaption and impacts of pre-exposure to pollution gradients, and of course development of mechanistic insights to connect the first encounter with NMs or MPs to an adverse outcome, via the key events in an adverse outcome pathway. Insights into these developments are presented below to inspire further advances and utilisation of these important organisms as part of an overall environmental risk assessment of NMs and MPs impacts, including in mixture exposure scenarios. Frontiers Media S.A. 2023-04-14 /pmc/articles/PMC10140508/ /pubmed/37124970 http://dx.doi.org/10.3389/ftox.2023.1178482 Text en Copyright © 2023 Reilly, Ellis, Davoudi, Supian, Maia, Silva, Guo, Martinez and Lynch. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Toxicology
Reilly, Katie
Ellis, Laura-Jayne A.
Davoudi, Hossein Hayat
Supian, Suffeiya
Maia, Marcella T.
Silva, Gabriela H.
Guo, Zhiling
Martinez, Diego Stéfani T.
Lynch, Iseult
Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways
title Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways
title_full Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways
title_fullStr Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways
title_full_unstemmed Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways
title_short Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways
title_sort daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways
topic Toxicology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140508/
https://www.ncbi.nlm.nih.gov/pubmed/37124970
http://dx.doi.org/10.3389/ftox.2023.1178482
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