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Biochemical basis for the formation of organ-specific volatile blends in mint

Above-ground material of members of the mint family is commercially distilled to extract essential oils, which are then formulated into a myriad of consumer products. Most of the research aimed at characterizing the processes involved in the formation of terpenoid oil constituents has focused on lea...

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Autores principales: Lange, B. Markus, Srividya, Narayanan, Lange, Iris, Parrish, Amber N., Benzenberg, Lukas R., Pandelova, Iovanna, Vining, Kelly J., Wüst, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140540/
https://www.ncbi.nlm.nih.gov/pubmed/37123862
http://dx.doi.org/10.3389/fpls.2023.1125065
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author Lange, B. Markus
Srividya, Narayanan
Lange, Iris
Parrish, Amber N.
Benzenberg, Lukas R.
Pandelova, Iovanna
Vining, Kelly J.
Wüst, Matthias
author_facet Lange, B. Markus
Srividya, Narayanan
Lange, Iris
Parrish, Amber N.
Benzenberg, Lukas R.
Pandelova, Iovanna
Vining, Kelly J.
Wüst, Matthias
author_sort Lange, B. Markus
collection PubMed
description Above-ground material of members of the mint family is commercially distilled to extract essential oils, which are then formulated into a myriad of consumer products. Most of the research aimed at characterizing the processes involved in the formation of terpenoid oil constituents has focused on leaves. We now demonstrate, by investigating three mint species, peppermint (Mentha ˣ piperita L.), spearmint (Mentha spicata L.) and horsemint (Mentha longifolia (L.) Huds.; accessions CMEN 585 and CMEN 584), that other organs – namely stems, rhizomes and roots – also emit volatiles and that the terpenoid volatile composition of these organs can vary substantially from that of leaves, supporting the notion that substantial, currently underappreciated, chemical diversity exists. Differences in volatile quantities released by plants whose roots had been dipped in a Verticillium dahliae-spore suspension (experimental) or dipped in water (controls) were evident: increases of some volatiles in the root headspace of mint species that are susceptible to Verticillium wilt disease (peppermint and M. longifolia CMEN 584) were detected, while the quantities of certain volatiles decreased in rhizomes of species that show resistance to the disease (spearmint and M. longifolia CMEN 585). To address the genetic and biochemical basis underlying chemical diversity, we took advantage of the newly sequenced M. longifolia CMEN 585 genome to identify candidate genes putatively coding for monoterpene synthases (MTSs), the enzymes that catalyze the first committed step in the biosynthesis of monoterpenoid volatiles. The functions of these genes were established by heterologous expression in Escherichia coli, purification of the corresponding recombinant proteins, and enzyme assays, thereby establishing the existence of MTSs with activities to convert a common substrate, geranyl diphosphate, to (+)-α-terpineol, 1,8-cineole, γ-terpinene, and (–)-bornyl diphosphate, but were not active with other potential substrates. In conjunction with previously described MTSs that catalyze the formation of (–)-β-pinene and (–)-limonene, the product profiles of the MTSs identified here can explain the generation of all major monoterpene skeletons represented in the volatiles released by different mint organs.
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spelling pubmed-101405402023-04-29 Biochemical basis for the formation of organ-specific volatile blends in mint Lange, B. Markus Srividya, Narayanan Lange, Iris Parrish, Amber N. Benzenberg, Lukas R. Pandelova, Iovanna Vining, Kelly J. Wüst, Matthias Front Plant Sci Plant Science Above-ground material of members of the mint family is commercially distilled to extract essential oils, which are then formulated into a myriad of consumer products. Most of the research aimed at characterizing the processes involved in the formation of terpenoid oil constituents has focused on leaves. We now demonstrate, by investigating three mint species, peppermint (Mentha ˣ piperita L.), spearmint (Mentha spicata L.) and horsemint (Mentha longifolia (L.) Huds.; accessions CMEN 585 and CMEN 584), that other organs – namely stems, rhizomes and roots – also emit volatiles and that the terpenoid volatile composition of these organs can vary substantially from that of leaves, supporting the notion that substantial, currently underappreciated, chemical diversity exists. Differences in volatile quantities released by plants whose roots had been dipped in a Verticillium dahliae-spore suspension (experimental) or dipped in water (controls) were evident: increases of some volatiles in the root headspace of mint species that are susceptible to Verticillium wilt disease (peppermint and M. longifolia CMEN 584) were detected, while the quantities of certain volatiles decreased in rhizomes of species that show resistance to the disease (spearmint and M. longifolia CMEN 585). To address the genetic and biochemical basis underlying chemical diversity, we took advantage of the newly sequenced M. longifolia CMEN 585 genome to identify candidate genes putatively coding for monoterpene synthases (MTSs), the enzymes that catalyze the first committed step in the biosynthesis of monoterpenoid volatiles. The functions of these genes were established by heterologous expression in Escherichia coli, purification of the corresponding recombinant proteins, and enzyme assays, thereby establishing the existence of MTSs with activities to convert a common substrate, geranyl diphosphate, to (+)-α-terpineol, 1,8-cineole, γ-terpinene, and (–)-bornyl diphosphate, but were not active with other potential substrates. In conjunction with previously described MTSs that catalyze the formation of (–)-β-pinene and (–)-limonene, the product profiles of the MTSs identified here can explain the generation of all major monoterpene skeletons represented in the volatiles released by different mint organs. Frontiers Media S.A. 2023-04-14 /pmc/articles/PMC10140540/ /pubmed/37123862 http://dx.doi.org/10.3389/fpls.2023.1125065 Text en Copyright © 2023 Lange, Srividya, Lange, Parrish, Benzenberg, Pandelova, Vining and Wüst https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lange, B. Markus
Srividya, Narayanan
Lange, Iris
Parrish, Amber N.
Benzenberg, Lukas R.
Pandelova, Iovanna
Vining, Kelly J.
Wüst, Matthias
Biochemical basis for the formation of organ-specific volatile blends in mint
title Biochemical basis for the formation of organ-specific volatile blends in mint
title_full Biochemical basis for the formation of organ-specific volatile blends in mint
title_fullStr Biochemical basis for the formation of organ-specific volatile blends in mint
title_full_unstemmed Biochemical basis for the formation of organ-specific volatile blends in mint
title_short Biochemical basis for the formation of organ-specific volatile blends in mint
title_sort biochemical basis for the formation of organ-specific volatile blends in mint
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140540/
https://www.ncbi.nlm.nih.gov/pubmed/37123862
http://dx.doi.org/10.3389/fpls.2023.1125065
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