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Cyclosporine Affects the Main Virulence Factors of Cryptococcus neoformans In Vitro
This study aimed to investigate the effects of cyclosporine on the morphology, cell wall structure, and secretion characteristics of Cryptococcus neoformans. The minimum inhibitory concentration (MIC) of cyclosporine was found to be 2 µM (2.4 µg/mL) for the H99 strain. Yeast cells treated with cyclo...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140927/ https://www.ncbi.nlm.nih.gov/pubmed/37108941 http://dx.doi.org/10.3390/jof9040487 |
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author | de Andrade, Iara Bastos Corrêa-Junior, Dario Alves, Vinicius Figueiredo-Carvalho, Maria Helena Galdino Santos, Marcos Vinicius Almeida, Marcos Abreu Valdez, Alessandro Fernandes Nimrichter, Leonardo Almeida-Paes, Rodrigo Frases, Susana |
author_facet | de Andrade, Iara Bastos Corrêa-Junior, Dario Alves, Vinicius Figueiredo-Carvalho, Maria Helena Galdino Santos, Marcos Vinicius Almeida, Marcos Abreu Valdez, Alessandro Fernandes Nimrichter, Leonardo Almeida-Paes, Rodrigo Frases, Susana |
author_sort | de Andrade, Iara Bastos |
collection | PubMed |
description | This study aimed to investigate the effects of cyclosporine on the morphology, cell wall structure, and secretion characteristics of Cryptococcus neoformans. The minimum inhibitory concentration (MIC) of cyclosporine was found to be 2 µM (2.4 µg/mL) for the H99 strain. Yeast cells treated with cyclosporine at half the MIC showed altered morphology, including irregular shapes and elongated projections, without an effect on cell metabolism. Cyclosporine treatment resulted in an 18-fold increase in chitin and an 8-fold increase in lipid bodies, demonstrating changes in the fungal cell wall structure. Cyclosporine also reduced cell body and polysaccharide capsule diameters, with a significant reduction in urease secretion in C. neoformans cultures. Additionally, the study showed that cyclosporine increased the viscosity of secreted polysaccharides and reduced the electronegativity and conductance of cells. The findings suggest that cyclosporine has significant effects on C. neoformans morphology, cell wall structure, and secretion, which could have implications for the development of new antifungal agents. |
format | Online Article Text |
id | pubmed-10140927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101409272023-04-29 Cyclosporine Affects the Main Virulence Factors of Cryptococcus neoformans In Vitro de Andrade, Iara Bastos Corrêa-Junior, Dario Alves, Vinicius Figueiredo-Carvalho, Maria Helena Galdino Santos, Marcos Vinicius Almeida, Marcos Abreu Valdez, Alessandro Fernandes Nimrichter, Leonardo Almeida-Paes, Rodrigo Frases, Susana J Fungi (Basel) Article This study aimed to investigate the effects of cyclosporine on the morphology, cell wall structure, and secretion characteristics of Cryptococcus neoformans. The minimum inhibitory concentration (MIC) of cyclosporine was found to be 2 µM (2.4 µg/mL) for the H99 strain. Yeast cells treated with cyclosporine at half the MIC showed altered morphology, including irregular shapes and elongated projections, without an effect on cell metabolism. Cyclosporine treatment resulted in an 18-fold increase in chitin and an 8-fold increase in lipid bodies, demonstrating changes in the fungal cell wall structure. Cyclosporine also reduced cell body and polysaccharide capsule diameters, with a significant reduction in urease secretion in C. neoformans cultures. Additionally, the study showed that cyclosporine increased the viscosity of secreted polysaccharides and reduced the electronegativity and conductance of cells. The findings suggest that cyclosporine has significant effects on C. neoformans morphology, cell wall structure, and secretion, which could have implications for the development of new antifungal agents. MDPI 2023-04-18 /pmc/articles/PMC10140927/ /pubmed/37108941 http://dx.doi.org/10.3390/jof9040487 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Andrade, Iara Bastos Corrêa-Junior, Dario Alves, Vinicius Figueiredo-Carvalho, Maria Helena Galdino Santos, Marcos Vinicius Almeida, Marcos Abreu Valdez, Alessandro Fernandes Nimrichter, Leonardo Almeida-Paes, Rodrigo Frases, Susana Cyclosporine Affects the Main Virulence Factors of Cryptococcus neoformans In Vitro |
title | Cyclosporine Affects the Main Virulence Factors of Cryptococcus neoformans In Vitro |
title_full | Cyclosporine Affects the Main Virulence Factors of Cryptococcus neoformans In Vitro |
title_fullStr | Cyclosporine Affects the Main Virulence Factors of Cryptococcus neoformans In Vitro |
title_full_unstemmed | Cyclosporine Affects the Main Virulence Factors of Cryptococcus neoformans In Vitro |
title_short | Cyclosporine Affects the Main Virulence Factors of Cryptococcus neoformans In Vitro |
title_sort | cyclosporine affects the main virulence factors of cryptococcus neoformans in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140927/ https://www.ncbi.nlm.nih.gov/pubmed/37108941 http://dx.doi.org/10.3390/jof9040487 |
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